A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF PARACETAMOL IN PHARMACEUTICAL DOSAGE FORM BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY


Mohan Thippeswamy, Prabhakara Somanna, Pavithra Krishnan, Basavaraj Bhandare, Sahajanand H

Abstract


Objective: An accurate, simple, reproducible and sensitive method for the determination of paracetamol in pharmaceutical dosage form was developed and validated using a reversed-phase C18 column (250 mm X 4.6 mm i. d, 5 µm particle size) with isocratic elution.

Methods: A mixture of Acetonitrile: 10 mM potassium dihydrogen orthophosphate buffer (15:85 v/v), pH 2.5 was used as a mobile phase at the flow rate of 1.0 ml/min and detector wavelength at 210 nm. The retention time of paracetamol was found to be 5.7 minutes (min). The method was statistically validated for the linearity, accuracy, precision and robustness.

Results: The linearity of paracetamol was in the range of 25.00 to 60.00µg/ml. This method showed an excellent linear response with the correlation coefficient (R2) value of 0.999 for the paracetamol. The recovery of the drug was ranged from 99.51 to 100.68%. An intra-day and inter-day precision study of the new method was less than the maximum allowable limit (% RSD<2.0).

Conclusion: The proposed method was cost effective, which can be used for the estimation of paracetamol in bulk and in solid dosage forms.

 


Keywords


Paracetamol, RP-HPLC, Isocratic, Retention time, Validation

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About this article

Title

A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF PARACETAMOL IN PHARMACEUTICAL DOSAGE FORM BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Keywords

Paracetamol, RP-HPLC, Isocratic, Retention time, Validation

Date

20-06-2015

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 7, Issue 8, 2015 Page: 190-194

Online ISSN

0975-1491

Statistics

230 Views | 108 Downloads

Authors & Affiliations

Mohan Thippeswamy
Central Research Laboratory, Raja Rajeswari Medical College and Hospital, Mysore Road, Bangalore 560074
India

Prabhakara Somanna
Central Research Laboratory, Raja Rajeswari Medical College and Hospital, Mysore Road, Bangalore 560074
India

Pavithra Krishnan
Department of Pharmacology, Raja Rajeswari Medical College and Hospital, Mysore Road, Bangalore 560074
India

Basavaraj Bhandare
Department of Pharmacology, Raja Rajeswari Medical College and Hospital, Mysore Road, Bangalore 560074
India

Sahajanand H
Department of Anaesthesia, Raja Rajeswari Medical College and Hospital, Mysore Road, Bangalore 560074
India


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