STUDIES ON THE EFFECT OF ANTIDIABETIC ACTIVITY OF ACHYRANTHES ASPERA L. ON ALLOXAN INDUCED WISTAR RATS
Objective: The present study was designed to investigate the effect of anti-diabetic activity of Achyranthes aspera on alloxan induced wistar rats.
Methods: Diabetes was induced by administration of alloxan mono hydrate (150 mg/kg body weight i. p) to albino wistar rats. Diabetic rats were stabilized for four days and from fifth-day aqueous extract of A. aspera were administered at the dose of 250 mg/kg and 500 mg/kg for 45 days. Metformin 1 mg/kg was used as a standard. The effects of A. aspera and standard drug on following parameters was recorded-fasting blood glucose, glycogen, plasma insulin, glycosylated hemoglobin and protein were analyzed in blood samples. Glucose-6-phosphatase, Glucose-6 phosphate dehydrogenase, tissue protein, reduced glutathione, and lipid peroxide were estimated in liver tissues.
Results: Our results collectively suggested that administration of aqueous extracts of A. aspera considerably lower the blood glucose level which was comparable to standard anti-diabetic drug metformin (1 mg). Also, the extract shows that considerable increase (pË‚0.05) in glucokinase activity when compared to untreated diabetic rats. Lipid peroxidation and reduced glutathione (GSH) level were also studied and the A. aspera aqueous extract and metformin-treated groups shows that the significant (pË‚0.05) reduction in lipid peroxide and marked elevation in reduced glutathione levels.
Conclusion: Aqueous extract of A. aspera possess anti-diabetic action in alloxan induced diabetic rats.
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