STUDIES ON THE EFFECT OF ANTIDIABETIC ACTIVITY OF ACHYRANTHES ASPERA L. ON ALLOXAN INDUCED WISTAR RATS

  • K Kamalakkannan Department of Biotechnology, Nandha College of Pharmacy, Erode 638052, Tamil Nadu, India
  • Balakrishnan V K. S. Rangasamy College of Technology

Abstract

Objective: The present study was designed to investigate the effect of anti-diabetic activity of Achyranthes aspera on alloxan induced wistar rats.

Methods: Diabetes was induced by administration of alloxan mono hydrate (150 mg/kg body weight i. p) to albino wistar rats. Diabetic rats were stabilized for four days and from fifth-day aqueous extract of A. aspera were administered at the dose of 250 mg/kg and 500 mg/kg for 45 days. Metformin 1 mg/kg was used as a standard. The effects of A. aspera and standard drug on following parameters was recorded-fasting blood glucose, glycogen, plasma insulin, glycosylated hemoglobin and protein were analyzed in blood samples. Glucose-6-phosphatase, Glucose-6 phosphate dehydrogenase, tissue protein, reduced glutathione, and lipid peroxide were estimated in liver tissues.

Results: Our results collectively suggested that administration of aqueous extracts of A. aspera considerably lower the blood glucose level which was comparable to standard anti-diabetic drug metformin (1 mg). Also, the extract shows that considerable increase (p˂0.05) in glucokinase activity when compared to untreated diabetic rats. Lipid peroxidation and reduced glutathione (GSH) level were also studied and the A. aspera aqueous extract and metformin-treated groups shows that the significant (p˂0.05) reduction in lipid peroxide and marked elevation in reduced glutathione levels.

Conclusion: Aqueous extract of A. aspera possess anti-diabetic action in alloxan induced diabetic rats.

Keywords: Achyranthes aspera, Anti-diabetic, Blood glucose, Glucokinase, Glycosylated hemoglobin

Downloads

Download data is not yet available.

References

1. Papadakis MA, Tierney LM, Mcphee SJ. Current medical diagnosis and treatment. International edition. Newyork: Lange medical books/Mc Graw-Hill; 2002. p. 1203-15.
2. Davis SN. Insulin, oral hypoglycemic agents and the pharmacology of the endocrine pancreas. In: Brunton LL. editors. Goodman and gilmans the pharmacological basis of therapeutics. New York: McGraw-Hill; 2006. p. 1613-45.
3. Bailey CJ. New pharmacological agents for diabetes. Curr Diabetes Rep 2001;1:119-26.
4. Bunn HG, Gabby KH, Gallop PM. The glycosylation of hemoglobin: relevance to diabetes mellitus. Science 1978;200:21-7.
5. Raghuram RA, Goverdhan RP, Venkateshwarlu E, Srinivas N, Ramya CH, Nirmala D. Anti-diabetic and hypolipidemic effect of Acalypha indica in streptozotocin nicotinamide Induced type-II diabetic rats. Int J Pharm Pharm Sci 2012;4:205-12.
6. Rai MK. A review on some anti-diabetic plants of India. Ancient Sci Life 1995;14:42-54.
7. Saurabh S, Pradeep S, Garima M, Jha KK, Khosa RL. Achyranthes aspera-An important medicinal plant: A review. J Nat Prod Plant Resour 2011;1:1-14.
8. Auniappan A, Savarimuthu I. Medicinal uses and pharmacological actions of five commonly used Indian medicinal plants: a mini-review. Iran J Pharmacol Ther 2008;7:107-14.
9. Khurana SMP, Bhargava KS. Effect of plant extracts on the activity of three papaya viruses. J Gen Appl Microbiol 1970;16:225-30.
10. Ikram M, Haq I. Screening of medicinal plants for antimicrobial activity. Fitoterapia 1980;51:281-4.
11. Londonkar R, Reddy CV, Kumar AK. Potential antibacterial and antifungal activity of Achyranthes aspera L. Recent Res Sci Technol 2011;3:53-7.
12. Pakrashi A, Basak B, Mokerji N. Search for antifertility agents from indigenous medicinal plants. Indian J Med Res 1975;63:375-8.
13. Akhtar MS, Iqbal J. Evaluation of the hypoglycaemic effect of Achyranthes aspera in normal and alloxan diabetic rabbits. J Ethnopharmacol 1991;31:49-57.
14. Kapoor VK, Singh H. Investigation of Achyranthes aspera Linn. Indian J Pharm 1967;29:285-8.
15. Neogi NC, Garg RD, Rathor RS. Preliminary pharmacological studies on Achyranthine. Indian J Pharm 1970;32:43-6.
16. Szkudelski T. The mechanism of alloxan and streptozotocin action in cells of the rat pancreas. Physiol Res 2001;50:537-46.
17. Folin WU. Estimation of blood glucose. J Biol Chem 1918;38:106.
18. Caroll N, Longley R, Roe JH. The determination of glycogen in liver and muscle by anthrone reagent. J Biol Chem 1956;220:583-93.
19. Morgan CR, Lazarow A. Immunoassay of insulin: Two antibody systems, Plasma insulin levels in normal, sub-diabetic and diabetic rats. Diabetes 1963;12:115-26.
20. Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated hemoglobin. Clin Chim Acta 1981;109:267-74.
21. Brandstrup N, Kirk JE, Bruni C. Determination of hexokinase in serum in liver disease. Clin Chim Acta 1959;4:554-61.
22. King J. The dehydrogenase or oxidoreductase lactate dehydrogenase. In: Van D. editors. Practical clinical enzymology. Norstand Company Ltd; 1965. p. 83-95.
23. Cyrus HF, Yellapragada S. The colorimetric method of phosphorus. J Biol Chem 1925;66:375-400.
24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
25. Sedlak J, Lindsay RH. Estimation of total protein bound and non protein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968;25:192-205.
26. Ohkawa H, Ohishi N, Yagi K. Assay for lipidperoxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
27. Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabetic potential. J Ethnopharmacol 2002;81:81-100.
28. Shu YZ. Recent natural products based drug development: a pharmaceutical Industry perspective. J Nat Prod 1998;61:1053-71.
29. Swanton SK, Day C, Bailey CJ, Flatt PR. Traditional plants treatment for diabetes: Studies in normal and streptozocin diabetic mice. Diabetologia 1990;33:462-4.
30. Karuppusamy A, Thangaraj P. Antidiabetic activity of aqueous root extract of Merremia tridentata (L) Hall in streptozotocin induced diabetic rats. Asian Pac J Trop Med 2012;5(3):175-9.
31. Carabaa A, Ricart MD, Mor A, Guinovart JJ, Ciudad CJ. Role of AMP on the activation of glycogen synthase and phosphorylase by adenosine fructose and glutamine in rat hypocytes. J Biol Chem 1990;26:2724-32.
32. Graham TE. Glycogen: An overview of possible regulatory roles of the proteins associated with the granule. Appl Physiol Nutr Metab 2009;34:488–92.
33. Roesler WJ, Khanderwal RL. Quantitation of glycogen synthase and phosphorylase protein mouse liver correlation between enzymatic protein and enzymatic activity. Arch Biochem Biophys 1986;244:397-407.
34. Gupta BL, Nehal M, Baquer NZ. Effect of experimental diabetes on the activities of hexokinase, glucose-6-phosphate dehydrogenase and catecholamines in rat erythrocyte, of different ages. Indian J Exp Biol 1997;35:792-5.
Statistics
383 Views | 925 Downloads
How to Cite
Kamalakkannan, K., and B. V. “STUDIES ON THE EFFECT OF ANTIDIABETIC ACTIVITY OF ACHYRANTHES ASPERA L. ON ALLOXAN INDUCED WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 61-64, https://innovareacademics.in/journals/index.php/ijpps/article/view/6527.
Section
Original Article(s)