HISTOLOGICAL STUDY ON PROTECTIVE EFFECT OF AQUATIC WEED HYDRILLA VERTICILLATA AGAINST LEAD INDUCED TOXICITY IN FISH

  • Pandi Prabha Department of Biotechnology, Sri Venkateswara college of Engineering, Post Bag No: 3, Pennalur, Sriperumbudur Taluk, Chennai 602117, Tamil Nadu, India
  • Johanna Rajkumar Department of Biotechnology, Rajalakshmi Engineering college, Thandalam, Chennai 602105, Tamil Nadu, India

Abstract

Objective: The main objective of this study is to reduce the lead induced toxicity in Labeo rohita using Hydrilla verticillata supplemented with normal fish food.

Methods: The fish were divided into three groups by treating with sublethal concentration of lead and feeding normal fish food, fish food supplemented with hydrilla dry powder and control fish without lead. After 21 days, the gill and liver tissue samples were collected and histological analysis was carried out.

Results: The histology of gill and liver of lead acetate induced toxicity against normal fish feed showed swollen gill, degeneration of lamella and hepatocytes and formation of cytoplasmic vacuoles. On the other hand, the histological changes were minimized in lead acetate induced toxicity against fish food supplemented with hydrilla and expressed apparently normal architecture.

Conclusion: Hence, it is proved that Hydrilla verticillata may be a very good keystone species to reduce lead toxicity in Labeo rohita.

 

Keywords: Hydrilla verticillata, Lead toxicity, Labeo rohita, Histology

Downloads

Download data is not yet available.

Author Biography

Pandi Prabha, Department of Biotechnology, Sri Venkateswara college of Engineering, Post Bag No: 3, Pennalur, Sriperumbudur Taluk, Chennai 602117, Tamil Nadu, India
Department of Biotechnology

References

1. Stephen S, Sanjeeva Raj PJ. Keystone functions of Hydrilla verticillata. Resonance 2013;18(2):156-62.
2. Sutton DL, Vandiver Jr VV, Hill JE. Grass carp: a fish for biological management of Hydrilla and other aquatic weeds in Florida. University of florida IFAS Extension: Bulletin; 2012. p. 867.
3. Pal DK, Nimse SB. Little known uses of common aquatic plant Hydrilla verticillata (Linn. f.) Royle. Nat Prod Resour 2006;5:108-11.
4. Lead and Lead Compounds, CAS NO.7439-92-1(Lead). Report on carcinogens. 12th edition. National Toxicology Program by US Department of Health and Human Services; 2011. p. 251-5.
5. US Environmental Protection Agency. Quality criteria for Lead. Washington: EPA-600/8-83/028 aF–dF; 1986.
6. World Health Organization (WHO), Lead: Environmental aspects. Geneva: Environmental Health Criteria; 1989. p. 85.
7. Department of Water Affairs and Forestry (DWAF). South African Water Quality Guidelines. 1st edition. Aquatic Ecosystem 1996;7:159–67.
8. Karthikeyan S. FTIR and ICP-AES study of the effect of heavy metals nickel and chromium in tissue protein of an edible fish Cirrhinus mrigala. Rom J Biophys 2012;22:95–105.
9. Ramani MB, Anna Mercy TV, Rajasekharan Nair J, Sherief PM. Changes in the proximate composition of Labeo rohita (Ham.) exposed to sublethal concentrations of monocrotophos. Indian J Fish 2002;49:427-32.
10. Schwaiger J, Wanke R, Adam S, Pawert M, Honnen W, Triebskorn R. The use of histopathological indicators to evaluate contaminant-related stress in fish. J Aquat Ecosyst Stress Recovery 1997;6:75-86.
11. Reddy PB, Waskale K. Using histopathology of fish as a protocol in the assessment of aquatic pollution, J Environ Res Dev 2013;8:371-5.
12. Bernet D, Schmidt H, Meier W, Burkhardt-Holm P, Wahli T. Histopathology in fish: proposal for a protocol to assess aquatic pollution. J Fish Dis 1999;22:25-34.
13. Kawser Ahmed Md, Parvin E, Monirul Islam Md, Salma Akter M, Shahneawz K, Habibullah Al-Mamun Md. Lead and cadmium induced histopathological changes in gill, kidney and liver tissue of freshwater climbing perch Anabas testudineus (Bloch, 1792). Chem Ecol 2014;30(6):1-9.
14. Kaoud HA, Dahshan AR. Bioaccumulation and histopathological alterations of the heavy metals in Oreochromis niloticus fish. Nat Sci 2010;8:147-56.
15. Mohapatra SB, Patra AK. Effect of partial replacement of fishmeal with duck weed (Lemna minor) feed on the growth performance of Cyprinus carpio Fry. IOSR J Agric Veterinary Sci 2013;4:34-7.
16. Palipoch S, Jiraungkoorskul W, Tansatit T, Preyavichyapugdee N, Jaikua W, Piya K. Protective efficiency of Thunbergia laurifolia leaf extract against lead (II) nitrate-induced toxicity in Oreochromis niloticus. J Med Plants Res 2011;5:719-28.
17. Royes JB, Chapman F. Preparing your own fish feeds. University of florida IFAS Extension: Cir 97(One of a series of the Fisheries and Aquatic Sciences Department); 2012.
18. US Environmental Protection Agency. Quality Criteria for Water. Washington; 1976.
19. US Environmental Protection Agency. Guidelines establishing test procedures for the analysis of pollutants. Washington; 1995.
20. Organization for Economic Co-operation and Development (OECD), OECD Guidelines for testing of chemicals. Paris: Test No: 203, Fish acute toxicity test; 1992.
21. Finney DJ. Probit Analysis. Cambridge, England: Cambridge University Press; 1952.
22. Finney DJ, Stevens WL. A table for the calculation of working probits and weights in probit analysis. Biometrika 1948;35:191-201.
23. Kim Suvarna S, Christopher L, Bancroft DJ. Bancroft’s Theory and practice of histological techniques. 7th ed. London: Churchill Livingstone; 2012.
24. Javid A, Javed M, Abdullah S, Ali Z. Bioaccumulation of lead in the bodies of major carps (Catla catla, Labeo rohita and Cirrhinus mrigala) during 96 h LC50 exposures. Int J Agric Biol 2007;9:909-12.
25. Evans DH, Piermarini PM, Choe KP. The multifunctional fish gill: Dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 2005;85:97-177.
26. Munshi Datta JS, Dutta Hiran M. Fish Morphology: Horizon of new research. USA: AA Balkema Publishers; 1996.
27. Wani Adil A, Sikdar-Bar M, Borana K, Khan HA, Andrabi SSM, Pervaiz PA. Histopathological alterations induced in gill epithelium of African catfish, Clarias gariepinus, exposed to copper sulphate. Asian J Exp Biol Sci 2011;2:278-82.
28. Nascimento AA, Araujo FG, Gomes ID, Mendes RM, Sales A. Fish gills alterations as potential biomarkers of environmental quality in a eutrophized tropical river in south-eastern Brazil. Anat Histol Embryol 2012;3:209-16.
29. Mallatt J. Fish gill structural changes induced by toxicants and other irritants: A statistical review. Can J Fish Aquat Sci 1985;42:630-48.
30. Dang ZC, Lock ACR, Flik G, Wendelaar bonga ES. Na+/K+-ATPase immunoreactivity in branchial chloride cells of Oreochromis mossambicus exposed to copper. J Exp Anim Sci 2000;203:379-87.
31. Kelly JM. Ecotoxicological assessment of juvenile northern pike inhabiting lakes downstream of a Uranium mill. Dissertation, University of Saskatchewan, Saskatoon; 2007.
32. Van der Oost R, Beber J, Vermeulen NPE. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 2003;13:57-149.
33. Naeemi AS, Jamili S, Shabanipour N, Sotoodeh MP, Salehzadeh A. Histopathological change induced in the liver of the Caspian kutum fry after acute exposure to the anionic surfactant. Eur J Zool Res 2013;2:100-5.
34. Rejeki S, Desrina D, Mulyana AR. Chronic affects of detergent surfactant (Linear Alkylbenzene Sulfonate/LAS) on the growth and survival rate of sea bass (Lates calcalifer Bloch), larvae. J Coastal Res 2008;8:207-26.
35. Srivastava S, Bhainsa KC, D'Souza SF. Investigation of uranium accumulation potential and biochemical responses of an aquatic weed Hydrilla verticillata (L. f.) Royle. Bioresour Technol 2012;101:2573-9.
36. Rahman AM, Hasegawa H. Aquatic arsenic: Phytoremediation using floating macrophytes. Chemosphere 2011;83:633–46.
37. Roy S. The mechanism of waterborne lead uptake and toxicity in Daphina Magna. Dissertation. University of Saskatchewan, Saskatoon; 2009.
38. Kandarat L, Wannee J, Somphong S, Tawewan T, Piya K, Raviporn M. Dietary calcium reducing effects of waterborne lead uptake in Nile Tilapia (Oreochromis niloticus). Asian J Anim Vet Adv 2007;2:104-14.
39. Peter VH, Beverley RB, Douglas JS, Keith A. Evaluation of erythrocyte δ-amino levulinic acid dehydratase activity as a short-term indicator in fish of a harmful exposure to lead. J Fish Res Board Can 1977;34:501-8.
40. Sevcikova M, Modra H, Slaninova A, Svobodova Z. Metals as a cause of oxidative stress in fish: a review. Vet Med 2011;56:537–46.
41. Lyn Patrick ND. Lead/Antioxidants. Altern Med Rev 2006;11:114-27.
42. Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biol Med 2000;29:927–45.
43. Sirimongkolvorakul S, Tansatit T, Preyavichyapugdee N, Kosai P, Jiraungkoorskul K, Jiraungkoorskul W. Efficiency of Moringa oleifera dietary supplement reducing lead toxicity in Puntius altus. J Med Plants Res 2005;62:187-94.
Statistics
330 Views | 2312 Downloads
How to Cite
Prabha, P., and J. Rajkumar. “HISTOLOGICAL STUDY ON PROTECTIVE EFFECT OF AQUATIC WEED HYDRILLA VERTICILLATA AGAINST LEAD INDUCED TOXICITY IN FISH”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 132-6, https://innovareacademics.in/journals/index.php/ijpps/article/view/6545.
Section
Original Article(s)