INHIBITORS FROM MELIA DUBIA AGAINST SDIA MEDIATED QUORUM SENSING OF UROPATHOGENIC E. COLI
Objective: To investigate the potentiality of Meliadubiastem extracts for quorum sensing (SdiA-selective) inhibitory activity against uropathogenic Escherichia coli (UPEC).
Methods: The antimicrobial (cell-density) and anti-virulent (swarming motility, protein, protease, hemolysis, hemagglutination, hydrophobicity and biofilm inhibition) properties of the Meliadubia stem extracts were performed.
Results: The biofilm, hemolysis, swarming motility were inhibited by 45.71%, 12.97 % and 33.33% respectively when the media were supplemented with 30 mg/ml of ethanolic extract. The GC-MS spectrum of ethanolic extract showed an array of 49 structurally unrelated compounds with the natural ligand, AHL. Their interaction with the quorum regulator, SdiA, was predicted by Glide module of SchrÃ¶dinger suite and the ligands C 7, C 20, C 28 showed appreciable activity with the following G-Score 11.4, 10.7, 9.9 respectively. In vitro and in silico molecular docking analysis data showed fairly good correlation, suggesting that the ethanolic extract has the potential to attenuate the quorum sensing of UPEC. Further investigation is desired to study the antagonistic effect of the above ligand by in vitro and in vivo strategies.
Conclusion: The quorum quenching activity of Meliadubia stem was proven from the overall analysis and its effect towards the inhibition of biofilm and virulence factors were analyzed.
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