• Nagwa E. Awad Pharmacognosy Department National Research Centre, 33 El-Bohouth St., Dokki, Cairo, Egypt. ID: 60014618
  • Mostafa A. Abdelkawy Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
  • Manal A. Hamed Therapeutic Chemistry Department National Research Centre, 33 El-Bohouth St., Dokki, Cairo, Egypt. ID: 60014618
  • Ahmed M. A. Souleman Phytochemistry and Plant Systematic Department, National Research Centre, 33 El-Bohouth St., Dokki, Cairo, Egypt. ID: 60014618
  • Enas H. Abdelrahman Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
  • Nehal S. Ramadan Pharmacognosy Department National Research Centre, 33 El-Bohouth St., Dokki, Cairo, Egypt. ID: 60014618


Objective: The antioxidant and hepatoprotective activities of ethyl acetate (EA) fraction of the dried aerial part of Justicia spicigera were evaluated and the characterization of its anthocyanin content was done.

Methods: Hepatic fibrosis was induced by carbon tetrachloride (CCl4) in rats. The ethyl acetate fraction was obtained by successive liquid/liquid fractionation of the crude cold ethanolic extract and the pigments were characterized by HPLC technique. The in vitro studies were carried out through evaluation of the EA fraction on the attenuation of 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. The in vivo biological evaluation was done in CCl4 injured rats through determination of liver function indices, oxidative stress markers and the histopathological picture of the treated liver.

Results: The phenolic content in the EA fraction was 42.94 mg/g. Twelve anthocyanins were identified, the major of which are peonidin 3, 5-diglucoside (64.30%), malvidin 3, 5-diglucoside (10.59%) and petunidin 3,5-diglucoside (4.71%). Treatment of CCl4 intoxicated rats with EA fraction recorded improvement in the liver function indices and oxidative stress markers. The histopathological observations confirmed our results.

Conclusion: The ethyl acetate fraction of the dried aerial part of Justicia spicigera recorded antioxidant and hepato protective activities.


Keywords: Justicia spicigera, Acanthaceae, Phenolic content, Anthocyanins, Oxidative stress, Histopathology


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How to Cite
Awad, N. E., M. A. Abdelkawy, M. A. Hamed, A. M. A. Souleman, E. H. Abdelrahman, and N. S. Ramadan. “ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS OF JUSTICIA SPICIGERA ETHYL ACETATE FRACTION AND CHARACTERIZATION OF ITS ANTHOCYANIN CONTENT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 91-96,
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