• M. S. Kalshetti Solapur University
  • R. Y. Patil Solapur University
  • R. A. Karale Department of Quality Assuarance, D. S. T. S. Mandal’s College of Pharmacy, Solapur University, Solapur, Maharashtra, India 413004
  • A. A. Kulkarni Solapur University


Objective: A rapid and selective high performance liquid chromatography (HPLC) method for estimation of Deferasirox in Human plasma was developed and validated.

Methods: Analyte was recovered by protein precipitation technique and subsequently separated on C18 column (150x4.5 mm, 5µ) by using acetonitrile: phosphate buffer pH 3.0(60:40) as mobile phase at a flow rate of 1.0 ml/min at 248 nm.

Results: The calibration curve was linear over the concentration range 0.4-4.8 µg/ml in human plasma. Within-batch and between-batch precision were<15% (at LLOQ<20%) and accuracy was all within 15%. (at LLOQ<20%).

Conclusion: The developed and validated HPLC method was specific, sensitive and reproducible which can be used for routine drug analysis and bioanalysis.


Keywords: HPLC-UV, Bioanalytical, Deferasirox, Human plasma


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How to Cite
Kalshetti, M. S., R. Y. Patil, R. A. Karale, and A. A. Kulkarni. “BIOANALYTICAL METHOD DEVELOPMENT FOR ESTIMATION OF DEFERASIROX IN HUMAN PLASMA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 399-02,
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