A VALIDATED UPLC-UV METHOD FOR BISPHENOLA (BP-A) LEVELS DETECTION IN IMPORTEDPLASTIC TOYS AND DRINKING BOTTLED WATER IN KUWAIT

  • Naser F Altannak Department of Pharmaceutical Chemistry Faculty of Pharmacy, Kuwait University, P. O Box 23924, Safat, 13110 Kuwait
  • Ali Alsaleh Faculty of Pharmacy- Kuwait University

Abstract

Objectives: Bisphenol A (BP-A) is an essential component of polyvinyl chloride, polystyrene, phthalates and polycarbonate plastics linked by ester bonds, and it can leach out of plastics at high temperature, acid and basic medium. BP-A is known to have an endocrine disrupting effect and recent studies have started to link its levels as causative factors in many diseases such as diabetes, obesity, and other endocrine diseases. Kuwait is considered one of the hottest countries in the world, and measurements of BP-A levels due to leakage from plastics have never been reported from this Middle-Eastern country. This study measures the levels of BP-A in four randomly selected plastic toys and two plastic water bottles from two different companies after storage at 45 °C for four days.

Methods: An ultra-pressure liquid chromatography coupled with an ultraviolet detector (UPLC-UV) analytical method was established to investigate BP-A levels in four of randomly chosen plastic toys (plastic tiger-plastic Lego blocks-plastic doll-small dolls) stored at 45 °C for 4 days. BP-A was extracted with 1 L of water and samples were analyzed by UPLC-UV.

Results: The limit of detection (LOD) and the limit of quantification (LOQ) of the established analytical method were equal to 0.4 ppb and 1 ppb, respectively. The analytical method was able to measure accurately and precisely traces of BP-A in all randomly selected toys. BP-A levels was239 ppb in plastic tiger, 30 ppb in plastic Lego, 4 ppb in plastic doll, 3 ppb in small dolls and 59 ppb in drinking bottled water.

Conclusion: The importance of BP-A level in plastics analysis raised due to its health concerns. Heat is a major factor for bisphenol leakage from plastics. However, Kuwait is considered one of the hottest countries, where high level leakage of BP-A from plastic toys and plastic water bottles for drinking water could pose significant health risks. Surprisingly, BP-A was detected in all randomly selected plastic toys and one out of two randomly selected polyethylene terephthalate (PET) drinking bottled water. Therefore, imported mineral water should be filled in a glass container rather than plastics due to high climate temperature. Moreover, Toys manufacturers should use BP-A free plastics or clearly specify storage conditions of their plastic products in order to prevent potential health risks resulting from BP-A leakage.

 

Keywords: Bisphenol A, Endocrine disruptor, Ultra Pressure Liquid Chromatography, Childhood obesity, Breast cancer, Altered pubertal development, Polyvinyl chloride, polystyrene, Phthalates, Polycarbonate plastics

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Author Biographies

Naser F Altannak, Department of Pharmaceutical Chemistry Faculty of Pharmacy, Kuwait University, P. O Box 23924, Safat, 13110 Kuwait

Assistant Professor

Department of Pharmaceutical Chemistry

Faculty of Pharmacy

Kuwait University

Kuwait

Ali Alsaleh, Faculty of Pharmacy- Kuwait University

5th year Pharmacy student

Depratment of Pharmaceutical Chemistry

Faculty of Pharmacy

References

1. Joe MB, Russ H. Bisphenol a and children’s health. Curr Opin Pediatr 2011;23(2):233-9.
2. Eertmans F, Dhooge W, Stuyvaert S, Comhaire F. Endocrine disruptors: effects on male fertility and screening tools for their assessment. Toxicol In Vitro 3003;17(5-6):515-24.
3. Beverly SR. Bisphenol A. An endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol 2011;127(1-2):27-34.
4. Welshons WV, Nagel SC, Vomsaal FS. Large effects from small exposures.×€×€×€. Endocrine mechanisms mediating effects of bisphenol A at levels of human exposure. Endocrinology 2006;147, Suppl 6:56-69.
5. Shankar A, Teppala S. Relation between urinary bisphenol A levels and diabetes mellitus. J Clin Endocrinol Metab 2011;96(12):3822-6.
6. Melzer D, Rice NE, Lewis C, Henley WE, Galloway TS. Association of urinary bisphenol A concentration with heart disease: evidence from NHANES 2003/06. PLoS One 2010;5(1):e8673.
7. Vandenberg LN, Maffini MV, Sonnenschein C, Rubin BS, Soto AM. Bisphenol-A and the great divide: a review of controversies in the field of endocrine disruption. Endocr Rev 2009;30(1):75-95.
8. Dodds EC, Lawson W. Synthetic estrogenic agents without the phenanthrene nucleus. Nature 1936;137(3476):996.
9. Cantonwine D, Meeker J, Hu H, Sanchez BN, Figueroa H, Garcia A, et al. Bisphenol a exposure in mexico city and risk of prematurity: a pilot nested case control study. Environ Health 2010;9(1):62.
10. Meeker JD, Ehrilich S, Toth TL, Wright DL, Calafat AM, Trisini AT, et al. Semen quality and sperm DNA damage in relation to urinary bisphenol A among men from an infertility clinic. Reprod Toxicol 2010;30(4);532-9.
11. Li DK, Zhou Z, Miao M, He Y, Wang J, Ferber J, et al. Urine bisphenol-A (BPA) level in relation to semen quality. Fertil Steril 2011;95(2):625-30.
12. Howdeshell KL, Hotchkiss AK, Thayer KA, Vandenbergh JG, Yom saal FS. Exposure to bisphenol A advances puberty. Nature 1999;401(6755):763-4.
13. Honma S, Suzuki A, Buchanan DL, Katsu Y, Watanabe H, Iguchi T. Low dose effect of in utero exposure to bisphenol A and diethylstilbestrol on female mouse reproduction. Reprod Toxicol 2002;16(2):117-22.
14. Zalko D, Jacques C, Duplan H, Bruel S, Perdu E. Viable skin efficiently absorbs and metabolizes bisphenol A. Chemosphere 2011;82(3):424-30.
15. Biedermann S, Tschudin P, Grob K. Transfer of bisphenol A from thermal printer paper to skin. Anal Bioanal Chem 2010;398(1):571-6.
16. Elobeid M, Almarhoon Z, Virk P, Hassan Z, Omer S, ElAmin M, et al. Bisphenol A detection in various brands of drinking bottled water in Riyadh, Saudi Arabia using gas chromatography/mass spectrometer. Trop J Pharm Res 2012;11(3):455-9.
17. Carvalho AR, Cardoso VV, Rodrigues A, Ferreira E, Benoliel MJ, Duarte EA. Occurrence and analysis of endocrine-disrupting compounds in a water supply system. Environ Monit Assess 2015;187(3):139-46.
18. Zhang L, Er J, Xu W, Qin X, Samanta A, Jana S, et al. “Orange alert”: A fluorescent detector for bisphenol A in water environments. Anal Chim Acta 2014;815:51-6.
19. http://en.wikipedia.org/wiki/Kuwait [Last accessed on 10 Jun 2013].
20. Richter CA, Birnbaum LS, Farabollini F, Newbold RR, Rubin BS, Talsness CE, et al. In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol 2007;24(2):199-224.
21. Braun JM, Yolton K, Dietrich KN, Hornung R, Ye X, Calafat AM, et al. Prenatal bisphenol A exposure and early childhood behavior. Environ Health Perspect 2009;117(12):1945-52.
22. WHO 2010, Joint FAO/WHO Expert Meeting to Review Toxicological and HealthAspects of Bisphenol A. Summary report; 2010. Available from http://www.who.int/ foodsafety/chem/chemicals/BPA_Summary2010. pdf?ua=1. [Last accessed on 10 Jun 2013].
23. LaKind JS, Naiman DQ. Daily intake of bisphenol a and potential sources of exposure: 2005-2006 national health and nutrition examination survey. J Exposure Sci Environ Epidemiol 2011;21(3):272-9.
24. Kim YH, Kim CS, Park S, Han SY, Pyo MY, Yang M. Gender differences in the level of bisphenol a metabolites in urine. Biochem Biophys Res Commun 2003;312(2):441-8.
25. Shao H, Han J, Hu J, Wu G, Xue Y, Ma Y, et al. Determination of alkylphenol and bisphenol A in beverages using liquid chromatography/electrospray ionization tandem mass spectrometry. Anal Chim Acta 2005;530:245-52.
26. Tokunaga OH, Liu X, Matsushima A, Shimohigashi Y. Direct evidence revealingstructural elements essential for the high binding ability of bisphenol A to human estrogen-related receptor-gamma. Environ Health Perspect 2008;116(1):32–8.
27. Amiridou D, Voutsa D. Alkylphenols and phthalates in bottled waters. J Hazard Mater 2011;185(1):281–6.
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How to Cite
Altannak, N. F., and A. Alsaleh. “A VALIDATED UPLC-UV METHOD FOR BISPHENOLA (BP-A) LEVELS DETECTION IN IMPORTEDPLASTIC TOYS AND DRINKING BOTTLED WATER IN KUWAIT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, July 2015, pp. 351-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/6743.
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