SCREENING OF PHYTO-CHEMICALS, TLC PROFILING, TOTAL FLAVONOID AND PHENOLICS CONTENT, ANTI-OXIDANT ACTIVITY AND ANTI-MICROBIAL ACTIVITY OF FICUS BENGHALENSIS LINN AND FICUS RELIGIOSA LINN LATEX
Objective: Screening for the presence of phyto-chemicals present in the plant extracts by qualitative assay along with Thin Layer Chromatography (TLC) investigation followed by determining the total flavonoids and phenolics content, anti-oxidant and anti-microbial effect.
Methods: Qualitative phyto-chemical analysis of the active plant extracts, TLC profiling, evaluating the total flavonoids and phenolics content along with in-vitro antioxidant activities like free radical scavenging effect, reducing power and phospho-molybdenum assay by standard protocols and evaluation of anti-microbial effectiveness against five different bacteria and a fungi by agar-well diffusion method. The micro-broth dilution method was used to assess minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Results: The solvent fractions of both the plants were examined for qualitative phytochemical analysis had shown the presence proteins, alkaloids, flavonoids, steroids, glycosides, phenolics compounds, tannins, and terpenoids, confirmed by TLC profiling. The antioxidant activity was high in methanol extract (lower Inhibitory Concentration, IC50 values) of both plants which were in accordance with the total phenolics and total flavonoids content showing that they were responsible for antioxidant activity. Microbial strains used in the study were exposed to various concentrations of four solvent plant extracts. The largest zone of inhibition was obtained with ethanol extract against E. coli (19 mm) when compared with standard antibiotic streptomycin (10 Âµg/ml) for bacteria and nystatin (10 Âµg/ml) for fungi and Dimethyl Sulfoxide-DMSO (negative control). The MIC and MBC values done in triplicates were in accordance with antimicrobial activity. The antimicrobial effect was in accordance with the presence of flavonoids which is responsible for inhibition of growth of pathogenic micro organisms.
Conclusion: The results suggested that the extract can be used as an effective and safe antioxidant source, as ethno-medicine on the commercial basis of drug development for the well being of human kind.
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