ANTI-ANXIETY EVALUATION OF EXTRACTS OF STIGMA MAYDIS (CORN SILK)
Objective: The anxiolytic activity of petroleum ether, chloroform and ethyl acetate extracts of Stigma maydis was investigated by Elevated Plus Maze, Hole Board and Mirror Chamber Test.
Methods: The study was conducted using elevated plus maze, whole board and mirror chamber test. Female Laca/Balb c was used to carry out the studies. In each experiment, animals were equally divided into five groups; control, given saline solution and Tween 80, standard given diazepam (2 mg/kg i. p.) and test groups were given 250, 500, 750 and 1000 mg/kg of petroleum ether, chloroform and ethyl acetate extracts of Stigma maydis. The data were subjected to analysis of variance by taking mean and standard error to the mean using Tukeyâ€™s post-hoc test.
Results: In Elevated Plus Maze chloroform extract (750 and 1000 mg/kg) of Stigma maydis revealed increase in time spent in open arm, frequency and preference to open arm as compared to control, which was almost comparable to diazepam. In Hole Board test decrease in number of head dips as compared to control was observed. In Mirror Chamber Test, the decrease in latency, increase in time spent in the mirror chamber and frequency as compared to control was observed. All of the changes were statistically highly significant.
Conclusion: From our results it can be concluded that the chloroform extract of Stigma maydis showed anxiolytic activity at the dose of 750 and 1000 mg/kg.
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