ANTI-ANXIETY EVALUATION OF EXTRACTS OF STIGMA MAYDIS (CORN SILK)
Objective: The anxiolytic activity of petroleum ether, chloroform and ethyl acetate extracts of Stigma maydis was investigated by Elevated Plus Maze, Hole Board and Mirror Chamber Test.
Methods: The study was conducted using elevated plus maze, whole board and mirror chamber test. Female Laca/Balb c was used to carry out the studies. In each experiment, animals were equally divided into five groups; control, given saline solution and Tween 80, standard given diazepam (2 mg/kg i. p.) and test groups were given 250, 500, 750 and 1000 mg/kg of petroleum ether, chloroform and ethyl acetate extracts of Stigma maydis. The data were subjected to analysis of variance by taking mean and standard error to the mean using Tukey's post-hoc test.
Results: In Elevated Plus Maze chloroform extract (750 and 1000 mg/kg) of Stigma maydis revealed increase in time spent in open arm, frequency and preference to open arm as compared to control, which was almost comparable to diazepam. In Hole Board test decrease in number of head dips as compared to control was observed. In Mirror Chamber Test, the decrease in latency, increase in time spent in the mirror chamber and frequency as compared to control was observed. All of the changes were statistically highly significant.
Conclusion: From our results it can be concluded that the chloroform extract of Stigma maydis showed anxiolytic activity at the dose of 750 and 1000 mg/kg.
2. Somers JM, Goldner EM, Waraich P, Hsu L. Prevalence and incidence studies of anxiety disorders: a systematic review of the literature. Am J Psychiatry 2006;2:100-12.
3. Donell JM, Shelton RC. Drug therapy of depression and anxiety disorders. In: Broton L, Chabner B, Knollman B. editors. Goodman and Gilmanâ€™s The Pharmacological Basis of Therapeutics. 12th ed. New York: Mc Graw Hill; 2011. p. 397-415.
4. Lader M, Morton S. Benzodiazepine problems. Br J Addict 1991;86:823-8.
5. Griffiths RR, Ator NA, Roache JD, Lamb RJ. Abuse liability of triazolam: experimental measurements in animals and humans. Psychopharmacol Res 1987;3:83-7.
6. Parle M, Dhamija I. Zea maize: A modern craze. Int Res J Pharm 2013;4(6):39-43.
7. Liu J, Lin S, Wang Z, Wang C, Wang E, Zhang Y, et al. Supercritical fluid extraction offlavonoids from Maydis stigma and its nitrite-scavenging ability. Food Bioprod Process 2011;89:333â€“9.
8. Ebrahimzadeh MA, Pourmorad F, Hafe S. Antioxidant activities of iranian corn silk. Turk J Biol 2008;32:43â€“9.
9. Hashim P. Corn silk (Stigma maydis) in healthcare: A phytochemical and pharmacological review. Molecules 2012;17:9697-715.
10. Steenkamp V. Phytomedicines for the prostate. Fitoterapia 2003;74:545â€“52.
11. Hu QL, Zhang LJ, Li YN, Ding YJ, Li FL. Purification and anti-fatigue activity of flavonoids from corn silk. Int J Phys Sci 2010;5:321â€“6.
12. Campo GM, Squadrito F, Ceccarelli S. Reduction of carbon tetrachloride-induced rat liver injury by IRFI 042, a novel dual vitamin E-like antioxidant. Free Radical Res 2001;34:379â€“93.
13. Kaup SR, Arunkumar N, Bernhardt LK, Vasari RG, Shetty SS, Pai SR, et al. Antihyperlipedemic activity of Cynodondactylon extract in high-cholestrol diet fed Wistar rats. Genomic Med Biomarkers Health Sci 2011;3:98â€“102.
14. Zhao W, Yin Y, Yu Z, Liu J, Chen F. Comparison of anti-diabetic effects of polysaccharides from corn silk on normal and hyperglycemia rats. Int J Biol Macromol 2012;50:1133â€“7.
15. Wang GQ, Xu T, Bu XM, Liu BY. Anti-inflammation effects of corn silk in a rat model of carrageenin-induced pleurisy. Inflammation 2011;35:822â€“7.
16. Habtemariam S. Extract of corn silk (Stigma of Zea mays) inhibit the tumour necrosis factor-Î±-and bacterial lipopolysaccharide-induced cell adhesion and ICAM-1 expression. Planta Med 1998;64:314â€“8.
17. Tian J, Chen H, Chen S, Xing L, Wang Y, Wang J. Comparative studies on the constituents, antioxidant and anticancer activities of extracts from different varieties of corn silk. Food Funct 2013;4(10):1526-34.
18. Bai H, Hai C, Xi M, Liang X, Liu R. Protective effect of maize silks (Maydis stigma) ethanol extract on radiation-induced oxidative stress in mice. Plant Foods Hum Nutr 2010;65:271â€“6.
19. Sepehri G, Derakhshanfar A, Zade FY. Protective effects of corn silk extract administration on gentamicin-induced nephrotoxicity in rat. Comp Clin Pathol 2011;20:89â€“94.
20. Ebrahimzadeh MA, Mahmoudi M, Ahangar N, Ehteshami S, Ansaroudi F, Nabavi SF, et al. Antidepressant activity of corn silk. Pharmacologyonline 2009;3:647â€“52.
21. Kulkarni SK, Reddy DS. Animal behavioral models for testing anti anxiety agents. Methods Find Exp Clin Pharmacol 1996;18:219â€“30.
22. Kulkarni SK. Practical Pharmacology and Clinical Pharmacy. 1st ed. Delhi: Vallabh Prakasan; 2009.
23. Brown RG, Nemes C. The exploratory behaviour of rats in the hole-board apparatus: Is head-dipping a valid measure of neophilia? Behav Processes 2008;78(3):442-8.
24. Moquin B, Blackman MR, Mitty E, Flores S. Complementary and alternative medicine (CAM). Geriatric Nursing 2009;30(3):196-203.
25. Dawson GR, Tricklebank MD. Use of the elevated plus maze in the search for novel anxiolytic agents. Trends Pharmacol Sci 1995;16:33-6.
26. Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67.
27. Takeda H, Tsuji M, Matsumiya T. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol 1998;350:21-9.
28. Griebel G, Perrault G, Tan S, Schoemaker H, Sanger DJ. Pharmacological studies on synthetic flavonoids: comparison with diazepam. Neuropharmacol 1999;38:965.
29. Paladini AC, Marder M, Viola H, Wolfman C, Wasowski C, Medina JH. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol 1999;51:519-26.