CHROMATOGRAPHIC ANALYSIS FOR DIOSGENINâ€ CONTENT IN ABUTILON INDICUM (L) SWEET
Objective: Chromatographic evaluation of steroidal saponin â€˜diosgenin' from leaves of the medicinal plant by HPTLC.
Methods: HPTLC analysis was performed with methanolic (MeOH) and ethanolic (EtOH) leaf extract of Abutilon indicum. HPTLC analysis was carried out with Toluene: ethyl acetate: formic acid [6:5:1, v/v/v] mobile phase and anisaldehyde-sulphuric acid reagent as spraying reagent for derivatization. Quantitative evaluation of diosgenin content in extracts was estimated using diosgenin standard curve (500-3000 ng/spot).
Results: HPTLC plate scanned at 366 nm showed good resolution and band color after derivatization. The appearance of brown color spot on chromatograms confirmed the diosgenin content in samples and it was observed to be 0.4 and 0.11 % (w/w) in MeOH and EtOH extracts, respectively.
Conclusions: A. indicum plant leaves are a good source of steroidal saponin diosgenin and can be used as an alternate natural source to synthesize herbal drugs to control human population, various tumor; oral contraceptives, sex hormones and other steroids. Further, the proposed HPTLC method is a faster, precise, accurate and cost effective, thus, help to the pharmaceutical companies for quantity, quality control and standardization of herbal formulations for routine analysis of diosgenin.
2. Giri RK, Kanungo SK, Patro VJ, Das S, Sahoo DC. Lipid lowering activity of Abutilon indicum (L.) leaf extracts in rats. J Pharm Res 2009;2:1725-7.
3. Khadabadi SS, Bhajipale NS. A review on some important medicinal plants of Abutilon spp. Res J Pharm Biol Chem Sci 2010;1:718-29.
4. Suresh G, Ganesana R, Dharmalingam M, Baskar S, Senthil PK. Evaluation of wound healing activity of Abutilon indicumâ€ Linn, in wister albino rats. Int J Adv Biol Biomed Res 2011;2:908â€“11.
5. Sharma A, Sharma RA, Singh H. Phytochemical and pharmacological profile of Abutilon Indicum L. sweet: a review. Int J Pharm Sci Rev Res 2013;20:120-7.
6. Chakraborthy GS. Antioxidant activity of Abutilon indicum Leaves. Int J PharmTech Res 2009;1:1314-6.
7. Krisanapun C, Peungvicha P, Temsiririrkkul R, Wongkrajang Y. Aqueous extract of Abutilon indicum Sweet inhibits glucose absorption and stimulates insulin secretion in rodents. Nutr Res 2009;29:579-87.
8. Dashputre NL, Naikwade NS. Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats. Int J Pharm Sci Drug Res 2011;3:97-100.
9. Kotnis MS, Patel P, Menon SN, Sane RT. Reneprotective effect of Hemisdesmus indicus a herbal drug used in gentomicin-induced renal toxicity. Nephrology (Carlton) 2004;3:142-52.
10. Hostettmann K, Marston A. Saponins. In: Chemistry and Pharmacology of Natural Products. Australia: Cambridge University Press; 1995. p. 584.
11. Yucekutlu AN, Bildaci I. Determination of plant saponins and some of Gypsophila species: a review of the literature. Hacettepe J Biol Chem 2008;36;129-35.
12. Sparg SG, Light ME, Van Staden J. Biological activities and distribution of plant saponins. J Ethnopharmacol 2004;94:219â€“43.
13. Shah HJ, Lele SS. Extraction of diosgenin, a bioactive compound from natural source Dioscorea alata Var purpurae. J Anal Bioanal Technol 2012;3:141-4.
14. Olayemi JO, Ajaiyeoba EO. Anti-inflammatory studies of yam (Dioscorea esculenta) extract on wistar rats. Afr J Biotechnol 2007;6:1913-5.
15. Hall JB, Walker DH. Balanitesa egyptiaca Del.-A Monograph: School of Agriculutural and Forest Science. Bange: University of Wales; 1991.
16. Raju J, Mehta R. Cancer chemopreventive and therapeutic effects of diosgenin, a food saponin. Nutr Cancer 2009;61:27-35.
17. Saxena R, Rathore SS, Barnwal P, Aditi Soni, Sharma L, Saxena SN. Effect of cryogenic grinding on recovery of diosgenin content in fenugreek (Trigonella foenum-graecum L.) genotypes. Int J Seed Spices 2013;3:26-30.
18. Kalyani R, Komal K, Shukla VJ, Prajapati PK. A study to evaluate Diosgeninâ€ in Laghu gokshur (T. terrestries) and Brihat gokshur (Pedalium murex) by HPTLC method. Int J Pharm Biol Archive 2012;3:1117-20.
19. Ghodasara T, Gaudani R, Nayak B, Shingala D. HPTLC method development and validation for the simultaneous estimation of diosgenin and gallic acid in the marketed formulation. Pharma Sci Monit 2012;3:2664-72.
20. Singh R, Mendhulkar VD. Abutilon indicum (Linn.) sweet leaves, a natural source of saponin: a spectrophotometric assay. Int J PharmTech Res 2015;8:725-9.
21. Laila O, Murtaza I, Abdin MZ, Ahmad S, Ganai NA, Jehangir M. Development and validation of HPTLC method for simultaneous estimation of diosgenin and quercetin in fenugreek seeds (Trigonella foenum-graceum). ISRN Chromatogr 2014;583047:1-8.
22. Kshirsagar VB, Deokate UA, Bharkad VB, Khadabadi SS. HPTLC method development and validation for the simultaneous estimation of diosgenin and levodopa in the marketed formulation. Asian J Res Chem 2008;1:36-9.
23. Hwang WT, Kim YS, Hong CK, Lee J, Park D. Simple stability-indicating validated HPLC method for diosgenin in cosmeceutical formulations with long-term stability application for light and heat. Int J Pharm Pharm Sci 2011;3:1-6.
24. Nyam MA, Wonang DL, Akueshi CO. Phytochemical screening and antimicrobial studies on Canarium schiveinfurthii Linn (Atiliâ€) fruits and oil. Nigerian J Bot 2009;22:247-53.
25. Mendhulkar VD, Kharat SN. HPTLC assay for quercetin and rutin flavonoids in Elephantopus scaber [Linn.] grown under induced heat stress condition. Int J Pharm Biol Sci 2015;6:(B)36â€“52.
26. ICH Harmonized Tripartite Guideline. Validation of Analytical Procedures: Text and Methodology. Q2 (R1); 2005.
27. Trivedi PD, Pundarikakshudu K, Rathnam S, Shah KS. A validated quantitative thin-layer chromatographic method for estimation of diosgenin in various plant samples, extract, and market formulation. J AOAC Int 2007;90:358â€“63.