• Manikandaselvi S. PG and Research Department of Biochemistry, S. T. E. T Women’s College, Mannargudi, Tamilnadu, India
  • David Raj C. SASTRA University
  • Aravind S. SASTRA University
  • Ravikumar R. Vivekanandha College of Arts and Sciences for Women
  • Thinagarbabu R. SASTRA University
  • Nandhini S. SASTRA University


Anemia, Phenyl hydrazine, Vigna radiata L, Bioferon


Objective: To evaluate the anti-anemic activity of sprouts of Vigna radiata L. against phenyl hydrazine induced anemic rats.

Methods: Rats were divided into 4 groups of 6 each. Group 1 was given normal saline and served as control and all other groups were given 40 mg/kg b. w of phenyl hydrazine for 2 d to induce anemia. Group 3 was treated with Bioferon (230 mg/kg) and served as the standard. Group 4 was treated with sprouted Vigna radiata L. (600 mg/kg bw). All the treatments were given orally. On completion of the experimental period, all the test substance/vehicle-treated rats were sacrificed and the plasma separated was used for estimating various biochemical as well as hematological parameters as per standard procedures.

Results: The experimental rats treated with sprouted Vigna radiata L. at the dose level 600 mg/kg bw for 13 d revealed significant changes in biochemical and hematological parameters compared to phenyl hydrazine induced anemic rats.

Conclusion: The present study concluded that the sprouted Vigna radiata L. inhibits anemia induced by phenyl hydrazine in male albino rats.



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Penninx BW, Guralnik JM, Onder G, Ferrucci L, Wallace RB, Pahor M. Anemia and decline in physical performance among older persons. Am J Med 2003;11:104–10.

Baker SJ, DeMaeyer EM. Nutritional anemia: its understanding and control with special reference to the work of the world health organization. Am J Clin Nutr 1979;3:368-417.

Tulchisky TH, Varavikova. New Public Health. 2ndedn. Elsevier Academic press: London. UK; 2009. p. 193-6.

Herifinda ET, Gourley DR. Textbook of therapeutics, drug and disease management. 6thedn. William and Wilkins, Baltimore, USA; 1996. p. 198-226.

Ravi U Thaker, Bhavin A Vyas, Shrikant V Joshi, Paras K Patel, Dinesh R Shah. Effect of dehydrated water extract of fruits of Opuntia ficus indica on experimentally induced hemolytic anemia in rats. Int J Pharm Res Dev 2012;4:185-91.

Min L. Research advance in chemical composition and pharmacological action of mung bean. Shanghai J Trad Chin Med 2001;5:18.

Fery RL. The cowpea: production, utilization, and research in the United States. Hortic Rev 1990;12:197–222.

Soobratte MA, Neergheen VS, Luximon-Ramma A. Phenolic as potential antioxidant therapeutic agents: mechanism and actions. Mutat Res 2005;579:200-13.

Nair RM, Yang RY, Easdown WJ, Thavarajah D, Thavarajah P, Hughes Jd, et al. Biofortification of mungbean (Vigna radiata) as a whole food to enhance human health. J Sci Food Agric 2013;8:1805-13.

Yang RY, Tsou SCS. Mungbean as a potential iron source in south Asian diets. In 35 International consultation workshop on mungbean. AVRDC: Shanhua; 1998. p. 152-8.

Wongekalak LSP, Jirasripongpun K, Hongsprabhas P. Potential use of antioxidativemungbean protein hydrolysate as an anti-cancer asiatic acid carrier. Food Res Int 2011;44:812–7.

Lai F, Wen Q, Li L, Wu H, Li X. Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment. Carbohydr Polym 2010;81:323–9.

Lee JH, Jeon JK, Kim SG, Kim SH, Chun T, Imm JY. Comparative analyses of total phenols, flavonoids, saponins and antioxidant activity in yellow soy beans and mung beans. Int J Food Sci Tech 2011;46:2513-9.

Kim JH, Lee BC, Kim JH, Sim GS, Lee DH, Lee KE, et al. The isolation and antioxidative effects of vitexin from acerpalmatum. Arch Pharm Res 2005;28:195–202.

Wang S, Rao P, Ye X. Isolation and biochemical characterization of a novel leguminous defense peptide with antifungal and antiproliferative potency. Appl Microbiol Biotechnol 2009;82:79–86.

Wang S, Shao B, Fu H, Rao P. Isolation of a thermostable legume chitinase and study on the antifungal activity. Appl Microbiol Biotechnol 2009;85:313–21.

Wang PF, Ye SY, Rao XY. Research progress on the biological activities and functions of mung beans. J Chin Inst Food Sci Technol 2004;1:26.

Ye XY, Ng TB. Mungin, a novel cyclophilin-like antifungal protein from the mung bean. Biochem Biophys Res Commun 2000;273:1111–5.

Wang S, Wu J, Rao P, Ng TB, Ye X. A chitinase with antifungal activity from the mung bean. Protein Expression Purif 2005;40:230–6.

Lee SJ, Lee JH, Lee HH, Lee S, Kim SH, Chun T, et al. Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages. Food Sci Biotechnol 2011;20:519–24.

Yeap SK, AliN M, Yusof HM, Noorjahan BA, Boon KB, Wan YH, et al. Antihyperglycemic effects of fermented and nonfermentedmung bean extracts on alloxan-induced-diabetic mice. BioMed Res Int 2012;1–7. 285430. [Article in Press]

Bellik Y, Hammoudi S, Abdellah F, Iguer-Ouada M, Boukraa L. Phytochemicals to prevent inflammation and allergy. Recent Pat Inflammation Allergy Drug Discovery 2012;6:147–58.

Cherng JM, Chiang W, Chiang LC. Immunomodulatory activities of edible beans and related constituents from soybean. Food Chem 2007;104:613–8.

Yao Y, Chen F, Wang M, Wang J, Ren G. Antidiabetic activity of mung bean extracts in diabetic KK-Ay mice. J Agric Food Chem 2008;56:8869–73.

Randhir R, Shetty K. Mung beans processed by solid-state bioconversion improves phenolic content and functionality relevant for diabetes and ulcer management. Innovative Food Sci Emerging Technol 2007;8:197–204.

Hsu GSW, Lu YF, Chang SH, Hsu SY. Antihypertensive effect of mung bean sprout extracts in spontaneously hypertensive rats. J Food Biochem 2011;35:278–88.

Matousek J, Podzimek T, Pouckova P, Stehlik J, Skvor J, Soucek J, et al. Antitumor effects and cytotoxicity of recombinant plant nucleases. Oncol Res 2009;18:163–71.

Xu B, Chang SK. Comparative study on antiproliferation properties and cellular antioxidant activities of commonly consumed food legumes against nine human cancer cells. Food Chem 2012;134:1287–96.

Zhao YR, Li ZW, Zhao C, Fu R, Wang XH, Li ZY. Effects of recombinant mung bean trypsin inhibitor fragments on migration of colon cancer cell SW480. J Shanxi Univ Nat Sci 2012;1:29.

Zhu S, Li W, Li JH, Arvin J, Andrew ES, Wang HC. It is not just folklore: the aqueous extract of mung bean coat is protective against sepsis. J Evidence-Based Complementary Altern Med 2012:1–10. [Article in Press]

Lee CH, Yoon SJ, Lee SM. Chlorogenic acid attenuates high mobility group Box 1 (HMGB1) and enhances host defense mechanisms in murine sepsis. Mol Med 2012;18:1437–48.

Kushwah A, Rajawat P, Kushwah HS. Nutritional evaluation of extruded Faba bean (Vicia faba L.) as a protein supplement in cereals based diet in rats. J Exp Biol 2002;40:49–52.

El-Adawy T, Rahma E, El-Bedawey A, El-Beltagy A. Nutritional potential and functional properties of germinated mung bean, pea and lentil seeds. Plant Foods Hum Nutr 2003;58:1–13.

Golob P. The use of spices and medicinals as bioactive protectants for grains. Rome: FAO Agricultural Sciences Bulletin; 1999. p. 137.

Diallo A, Gbeassor M, Ahoefa Vovor B, Kwashie Eklu-Gadegbeku A, Kodjo Aklikokou A, Amegnona Agbonon A, et al. Effect of Tectona grandis on phenylhydrazine-induced anemia in rats. Fitoterapia 2008;79:332-6.

Dacie JV, Lewis SM. Practical Hematology. 4th edition. Churchhill, UK; 1968. p. 37.

OcheiJ, Kolhatkar A. Medical laboratory science theory and practice. Tata McGraw-Hill Publishing Company Limited, New Delhi; 2000. p. 281-7.

Beuge JA, Aust SD. The Thiobarbituric acid assay. Methods in enzymology 1978;52:306-7.

Moron MS, Dsepierre JW, Manerwik KB. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biophysica Acta 1979;582:67-8.

Reitman S, Frankel SA. Colorimetric method for the determination of serum Glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 1957;25:56-63.

Harvey J, Paige SM. The In stat guide to choosing and interpreting statistical tests: a manual for graph pad Instat San Diego, CA USA; 1998.

Akyüz M. Nutritive value, flavonoid content and radical scavenging activity of the truffle. J Soil Sci Plant Nutr 2013;13:143-51.

Gannet PM, Lawson TS, Kolar C, Toth B. Aryl radical formation during the metabolism of aryl hydrazine by microsomes. Chem Res Toxicol 1997;10:1372-7.

Prasad AS. Zinc and trace minerals. Workshop on nutrient metabolism in genetic anemia, Bethesoda, USA, NHLBI; 1999.

Ferrali M, Signorini C, Pompella A, Lodovic M, Caciotti B, Ciccoli L, et al. Release of free redox-active iron in the liver and DNA oxidative damage following phenyl hydrazine intoxication. Biochem Pharmacol 1997;53:1743-51.

Criswell KA, Sulkanen AP, Hochbaum AF, Bleavins MR. Effects of phenylhydrazine or phlebotomy on peripheral blood, bone marrow and Erythropoietin in Wistar rats. J Appl Toxicol 2000;20:25-34.

Andre Muller, Helene Jacobsen, Edel Healy, Sinead McMickan, FréderiqueIstace, Marie-Noëlle Blaude, et al. Hazard classification of chemicals inducing hemolytic anemia: an EU regulatory perspective. Regul Toxicol Pharmacol 2006;45:229-41.

Shukla S, Mehta A, John J, Singh S, Mehta P, Vyas S. Clinical manifestations of human parvovirus B19 in adults. Arch Int Med 2012;149:11532-6.

Tyurina YY, Shvedova AA, Kanai K, Tyurin VA, Kommineni C, Quinn PJ, et al. Phospholipid signaling in apoptosis. Peroxidation and externalization of Phosphatidylserine. Toxicology 2000;148:93–101.

Hochstein P. Perspectives on hydrogen peroxide and drug-induced hemolytic anemia in glucose-6-phosphate dehydrogenase deficiency. Free Radical Biol Med 1988;5:387–92.

McMillan DC, Powell CL, Bowman ZS, Morrow JD, Jollow DJ. Lipids versus proteins as major targets of per-oxidant, direct-acting hemolytic agents. Toxicol Sci 2005;88:274-83.

Haram K, Nilsen ST, Ulvik RJ. Iron supplementation in pregnancy--evidence and controversies. Acta Obstet Gynecol Scand 2001;80:683-8.

Vyshtakaliuk AB, Zobov VV. Antianemic activity of water-soluble Na, Ca, Fe-polygalacturonate. Bull Exp Biol Med 2010;150:45-7.

Mittal MB, Abhay RS, Yadunath MJ, Vilasrao JK. An intervention on iron deficiency anemia and change in dietary behavior among adolescent girls. Int J Pharm Pharm Sci 2011;3:40-2.

Balaji K, Brindha P, Sridharan K, Uma Maheshwari K, Swaminathan S, Rajan KS. Elucidation of a core-shell model for Lauhabhasma through physico-chemical characterization. Int J Pharm Pharm Sci 2012;4:644-9.



How to Cite

S., M., D. R. C., A. S., R. R., T. R., and N. S. “ANTI-ANEMIC ACTIVITY OF SPROUTS OF VIGNA RADIATA L. IN MALE ALBINO RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 263-7,



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