CYTOTOXIC SCREENING OF THREE EGYPTIAN PLANTS USING BRINE SHRIMP LETHALITY TEST

  • Mosad A Ghareeb Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
  • Laila A Refahy Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
  • Amal M Saad Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
  • Maha A El-shazely Theodor Bilharz Research Institute
  • Asmaa S Mohamed Theodor Bilharz Research Institute
  • Nadia S Osman Theodor Bilharz Research Institute

Abstract

Objective: Brine shrimp (Artemia salina L.) bioassay is considered as a preliminary screening for the presence of antitumor compounds and used to determine the toxicity of plant extracts. Different extracts of three Egyptian plants, philodendron selloum (Araceae), Bougainvillea alba (Nyctaginaceae) and Ulmus parvifolia (Ulmaceae) were screened for their preliminary cytotoxicity.

Methods: The dry powdered leaves of the three plants under investigation (500 g from each plant) were extracted with 85% methanol; then undergo successive fractionation via petroleum ether, chloroform and ethyl acetate. The brine shrimp lethality test (BSLT) was carried out via taking 50 mg of Artemia salina Leach eggs in a hatching chamber and serial concentrations from each extract were used.

Results: The chloroform extract of Philodendron selloum showed the most potent cytotoxic activity at sub-lethal concentration (LC50=16 µg/ml) followed by its methanol extract (LC50=26 µg/ml). The methanol, chloroform and petroleum ether extracts of Bougainvillea alba plant showed the same cytotoxic effect at LC50= 40 µg/ml. Ulmus parvifolia showed the least cytotoxic effect at LC50 =380 & 300 µg/ml for methanol and ethyl acetate extracts respectively.

Conclusion: It may be concluded that the tested plants possess strong cytotoxic activies; also this work suggests that plants extracts could possibly be used locally as naturally occurring cytotoxic agents.

Keywords: Brine Shrimp, Artemia salina, Philodendron selloum, Bougainvillea alba, Ulmus parvifolia, cytotoxicity

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Author Biography

Mosad A Ghareeb, Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
Medicinal Chemistry Department, Theodor Bilharz Research Institute (TBRI)

References

1. Vanhaecke P, Persoone G, Claus C, Sorgeloos P. Proposal for a short-term toxicity test with Artemia salina Nauplii. Ecotoxicol Environ Saf 1981;5:382-7.
2. Sam TW. Toxicty testing using the brine shrimp: Artemia salina. In: Bioactive natural product: Detection, Isolation and Structure determination. 1st ed. Boca Rotan FL, USA CRC Press; 1993. p. 441-56.
3. Shoeb HA, Hassan MFM, Laila AR, Mona AM, Amal MS, Mosad AG. Antioxidant and cytotoxic activities of Gmelina arborea ROXB. leaves. Br J Pharm Res 2014;4:125-44.
4. Ghareeb MA, Hussein AS, Hassan MFM, Laila AR, Mona AM, Amal MS. Antioxidant and cytotoxic activities of Tectona grandis Linn. leaves. Int J Phytopharmacol 2014;5:143-57.
5. Nunes BS, Caivalho FD, Guilhermino LM, Van SG. Use of the genus Artemia salina in ecotoxicity testing. Environ Pollut 2006;14:453-62.
6. Mclaughlin JL. Crown gall tumors on potato discs and brine shrimp lethality: Two simple bioassays for higher plant screening and fractionation. In: Hostettmann K. ed. Methods in Plant Biochemistry. London Academic Press; 1991. p. 1-31.
7. Feitosa CM, Bezerra MZB, Citó AML, Soares da Costa JJ, Lopes JA, Neto JMM. Constituents químicos de Philodendron imbe Schott. Quím Nova 2007;30:41-4.
8. Heuer S, Richter S, Metzger JW, Wray V, Nimtz M, Strack D. Betacyanins from bracts of Bougainvillea glabra. Phytochemistry 1994;37:761-7.
9. Simon A, Tóth G, Duddeck H, Soliman HS, Mahmoud II, Samir H. Glycosides from Bougainvillea glabra. Nat Prod Res 2006;20:63-7.
10. Baker JE, NORRIS DM. A feeding stimulant for Scolytus multistriatus (Coleoptera: Scolytidae) isolated from the bark of Ulmus americana. Ann Entomol Soc Am 1967;60:1213-5.
11. Meyer HJ. Studies on the feeding and short range attractant behaviour of the smaller european elm bark beetle, Scolytus multistriatus (Marsh.) (Coleoptera: Scolytidae). Diss Abstr Int 1975;35:3958.
12. Kuo YH, Chang CI, Kuo YH. Triterpenes from Diospyros maritima. Phytochemistry 1997;46:1135-7.
13. Dumas MT, Strunz GM, Hubbes M, Jeng RS. Isolation and identification of six mansonones from Ulmus americana infected with Ceratocystis ulmi. Cell Mol Life Sci 1983;39:1089-90.
14. Overeem JC, Elgersma DM. Accumulation of masonones E and F in Ulmus Hollandica infected with Ceratocystis ulmi. Phytochemistry 1970;9:1949-52.
15. Ramesh P, Nair, AGR, Subramanian SS. Flavone glycosides of Vitex trifolia. Fitoterapia 198;57:282-3.
16. Preeti R, Manmeet K, Kunal S, Naibedya C, Rakesh M. Ulmosides A and B: Flavonoid 6-C-glycosides from Ulmus wallichiana, stimulating osteoblast differentiation assessed by alkaline phosphatase. Bioorg Med Chem Lett 2009;19:4684-7.
17. Kang S, Kim K, Byun Y, Suh S, Jim U, Kim K, et al. Effect of Ulmus davidiana planch6 on mineralization, bone morphogenetic protein-2, alkaline phosphatase, type 1 collagen, and collagenase-1 in bone cells in vitro. Cell Dev Biol Anim 2006;42:25-9.
18. Institutum Botanicum Academiae Sinicae, "Iconographia Cormophytorum."1st ed. Tomus I Science press, Beijing; 1972.
19. Jiang SU. New Medical College, "Chinese Crud Drug Dictionary." 1st ed. The last volum, Shanghai People press, Shanghai; 1977.
20. Wang D, Xia M, Cui Z. New triterpenoids isolated from the root bark of Ulmus pumila L. Chem Pharm Bull 2006;54:775-8.
21. Meyer BN, Ferrign NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. Brine shrimp: a convenient general bioassay for active constituents. Planta Med 1982;45:31-4.
22. Miya TS, Holck, HGO, Yim GKW, Mennear JH, Spratto GR. Laboratory Guide in Pharmacology. 4th ed. Burgess Publishing, Minneapolis; 1973. p. 1237.
23. Ipsen J, Feigi P. Bancroft's Introduction to Biostatistics. 2nd ed., Harper and Row. New York; 1970. p. 15.
24. Deciga-Campos M, Rivero-Cruz I, Arriaga-Alba M, Castaneda-Corral G, Angeles-Lopez GE, et al. Acute toxicity and mutagenic activity of mexican plants used in traditional medicine. J Ethnopharmacol 2007;110:334-42.
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How to Cite
Ghareeb, M. A., L. A. Refahy, A. M. Saad, M. A. El-shazely, A. S. Mohamed, and N. S. Osman. “CYTOTOXIC SCREENING OF THREE EGYPTIAN PLANTS USING BRINE SHRIMP LETHALITY TEST”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 507-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/7006.
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