• Sriram Sridharan Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, Thanjavur 613401, Tamil Nadu
  • Meenaa Venkatramani SASTRA University
  • Kavitha Janakiraman SASTRA University
  • Brindha Pemiah SASTRA University
  • Sasikumar Chinnagounder SASTRA University


Objective: To evaluate the anti-inflammatory activity of the ethanolic leaf extract of Barleria montana (B. montana) against formalin induced inflammation.

Methods: Male albino wistar rats were pretreated with oral doses of 100 mg, 200 mg and 300 mg of the extract for 30 days and the animals received a single dose of sub plantar injection of formalin (0.1 ml/kg body weight (bw.)) Indomethacin (25 mg/kg bw.) was used as the standard drug. The effect of the extract on paw thickness, biochemical and hematological parameters was investigated along with histopathological studies.

Results: The results revealed that the extract exhibited an effective dose dependent activity against formalin induced inflammation with a maximum activity at a dosage of 300 mg/kg bw. which was comparable with the standard drug.

Conclusion: The results signify promising activity of ethanolic leaf extract of B. montana against formalin induced inflammation in albino rats. The extract was also found to exhibit appreciable antioxidant activity.


Keywords: Barleria montana, Formalin, Inflammation, Indomethacin


Download data is not yet available.


1. Sosa S, Altinie G, Politi M, Braca A, Morelli I, Loggia RD. Extracts and constituents of Lavandula multifida with topical anti-inflammatory activity. Phytomedicine 2005;12:271-7.
2. Libby P. Inflammation in atherosclerosis. Nature 2002;420:868–74.
3. Dandona P, Aljada A, Bandyopadhyay A. Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol 2004;25:4–7.
4. Lisa Coussens M, Zena Werb Z. Inflammation and Cancer. Nature 2002;420:860-7.
5. Jing Liu J, Jianbo Wan J, Cheng Wei He C. Rationale for the use of natural anti-inflammatory agents in cancer chemotherapy. North Am J Med Sci 2010;3:160-6.
6. Brunton LL, Lazo JS, Parker LK. The Pharmacological Basis of Therapeutics. London: Medical publishers; 2006. p. 671-12.
7. Donihi AC, Raval Saul D, Korytkowsk MT, Devita MA. Prevalence and predictors of corticosteroid-related hyperglycemia in hospitalized patients. Endocr Pract 2006;12:358-62.
8. Wallace JL. Nonsteroidal anti-inflammatory drugs and gastroenteropathy: the second hundred years. Gastroenterology 1997;112:1000-16.
9. Shah BN, Seth AK, Maheshwari KM. A Review on medicinal plants as a source of anti-inflammatory agents. Res J Med Plant 2011;5:101-15.
10. Shanaz Banu S, Arunachalam G, Jayaveeera KN, Josephine Leno Jenita J, Ayesha Siddiqua A. Pharmacognostic studies of Barleria montana DUN. Leaves. World J Pharm Res 2012;1:332-9.
11. Joseph SM, George MC, Nair JR. Effect of feeding cuttlefish liver oil on immune function, inflammatory response and platelet aggregation in rats. Curr Sci 2005;88:507-10.
12. Duffy JC, Dearden JC, Rostron C. Design, Synthesis and biological testing of a novel series of anti-inflammatory drugs. J Pharm Pharmacol 2001;53:1505-14.
13. Beutler E, Duron C, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
14. Rotruck JT, Pope AL, Ganther H, Swanson AB, Hafeman DG, Hoeksira WG. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
15. Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for SOD. J Biol Chem 1972;247(5, Suppl 10):3170-5.
16. Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissues for thiobarbituric acid reaction. Annu Biochem 1979;95:351-8.
17. King J. In: Practical Clinical Enzymology, Princeton MJ (Fol) Van D Nostrand Company, London; 1965. p. 363.
18. Okinaka S, Kumagi Ebashi S, Sugita H, Morno H, Toyokura Y. Serum Creatine phosphokinase activity in progressive muscular dystrophy and neuromuscular disease. Arch Neurol 1961;4:520-5.
19. Armour D, Blood FR, Belden DA. The manual for laboratory work in mammalian Physiology. 3rd ed. Chicago: The university of Chicago press; 1964. p. 4-6.
20. Dacie J, Lewis S. Practical haematology. London: Churchill Livingstone; 1995.
21. Sujai Suneetha S. Histopathological Techniques, in Handbook of CMAI Medical Laboratory Technology by Robert H Carman. Christian Med Assoc India 1993;24:508-41.
22. Young H, Lu Y, Cheng H, Hsieh W, Lia J, Peng W. Analgesic and anti-inflammatory activities of Gingerol. J Ethanopharmacol 2005;96(1, Suppl 2):207-10.
23. Hassa MQ, Hadi RA, Al-Rawi ZS. The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol 2001;21:69-73.
24. Goel RK, Sairam K, Rao CH, Raman A. Role of gastric antioxidant and anti Helicobacter pylori activities in the anti ulcerogenic activity of banana. Indian J Exp Biol 2001;39:719-22.
25. Agnel Arul John N, Shobana G. Anti-inflammatory activity of Talinum fruticosum L. on formalin induced paw edema in albino rats. J Appl Pharm Sci 2012;2:123-7.
26. Hull MA, Gardner SH, Hawcroft G. Activity of the non-steroidal anti-inflammatory drug indomethacin against colorectal cancer. Cancer Treat Rev 2003;29:309–20.
27. Habila JD, Bello IA, Dzikwi AA, Musa H, Abubakar N. Total phenolics and antioxidant activity of Tridax procumbens Linn. Afr J Pharm Pharmacol 2010;4:123–6.
28. Saraswathi CD, Wagh SP, Kunal PW. Anti-arthritic activity of Morinda citrifolia L. fruit juice in Complete Freund’s adjuvant induced arthritic rats. J Pharm Res 2012;5:1236-9.
29. Apple FS, Murakami M, Panteghini M. International survey on the use of cardiac markers. Clin Chem 2001;47:587–8.
274 Views | 425 Downloads
How to Cite
Sridharan, S., M. Venkatramani, K. Janakiraman, B. Pemiah, and S. Chinnagounder. “BARLERIA MONTANA WIGHT AND NEES–A PROMISING NATURAL ANTI-INFLAMMATORY AGENT AGAINST FORMALIN INDUCED INFLAMMATION”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 80-84,
Original Article(s)