AMELIORATIVE EFFECTS OF ARTEMISIA JUDAICA L. EXTRACT AGAINST ALLOXAN-INDUCED BIOCHEMICAL ALTERATIONS IN MALE WISTAR RATS

  • Eman G.e. Helal Al-Azhar University
  • Nouran Abou Aouf Al-Azhar University
  • Hany Nady Yousef Al-Azhar University
  • Fatimah M Yousef Department of Food and Nutrition, Faculty of Home Economics, King Abdulaziz, University, Jeddah, Saudi Arabia
  • Al Sayeda Mohammad Khattab Department of Food and Nutrition, Faculty of Home Economics, King Abdulaziz, University, Jeddah, Saudi Arabia

Abstract

Objective: The current study aims to evaluate the potential of crude leaf extract of Artemisia judaica L. (AJE) in reducing the biochemical abnormalities accompanied to alloxan-induced diabetes in male Wistar rats.

Methods: Thirty male albino rats (100-110 g) were divided equally into three groups including control, diabetic and diabetic+AJE. Diabetes was induced by using a single dose of alloxan (120 mg/kg of body weight). Serum biochemical parameters, including insulin, glucose, triglycerides, total cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), very low density lipoprotein cholesterol (VLDL), total proteins, albumin, globulin, renal markers (creatinine, urea, uric acid), activities of aspartate transaminase (AST), alanine transaminase (ALT) and gamma-glutamyltransferase (ɤGT) were measured in all groups. Also, values of homeostasis model assessment of insulin resistance (HOMA-IR) and ratios of albumin: globulin (A: G), TC/HDL (risk factor-1), LDL/HDL (risk factor-2) were calculated for each group.

Results: Diabetic rats showed reduction in body weight and marked decline in the values of serum insulin, protein profile indices and HDL accompanied with marked elevation in values of glucose, HOMA_IR, triglycerides, TC, LDL, VLDL, TC/HDL, LDL/HDL, renal markers and activities of the estimated enzymes. Supplementation of diabetic rats with AJE, twice daily for 30 days, significantly ameliorated most of the estimated biochemical parameters.

Conclusion: The current results demonstrate that AJE possesses a hypoglycemic effect and acts as a protective factor against metabolic abnormalities induced by diabetes mellitus.

 

Keywords: Artemisia judaica, Alloxan, Diabetes, Lipid profile, Renal markers, Hyperglycemia

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Helal, E. G., N. A. Aouf, H. N. Yousef, F. M. Yousef, and A. S. M. Khattab. “AMELIORATIVE EFFECTS OF ARTEMISIA JUDAICA L. EXTRACT AGAINST ALLOXAN-INDUCED BIOCHEMICAL ALTERATIONS IN MALE WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 90-94, https://innovareacademics.in/journals/index.php/ijpps/article/view/7038.
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