PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF CORIANDRUM SATIVUM (L) SEEDS

  • Arun Thangavel Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram 637408, Tamil Nadu, India
  • Senthil Kumar Balakrishnan Muthayammal College of Arts and Science Haramaya University
  • Vijayalakshmi . Muthayammal College of Arts and Science
  • Senbagam Duraisamy Vivekananda College of Arts and Science for Women

Abstract

Objective: The present study was carried out to evaluate the phytochemical screening, Gas Chromatography–Mass Spectrometry analysis of phyto-constituents and antibacterial activity of Coriandrum sativum (L.) seeds against the bacterial [Microbial Type Culture Collection (MTCC)] strains.

Methods: Methanol, ethanol, ethyl acetate, acetone and water extracts of Coriandrum sativum (L) seeds were prepared using the Soxhlet apparatus. The antibacterial activity of various extracts of C. sativum seeds were identified by disc diffusion method against bacterial strains. The bioactive components of methanol and acetone fraction of C. sativum seeds were evaluated by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The minimal inhibitory concentration (MIC) values were obtained by the well diffusion method between the ranges of 1000µg/ml to 7.8µg/ml.

Results: The preliminary phytochemical screening of various extracts of C. sativum revealed the presence of different Phyto-constituents. Methanol extract of C. sativum showed the maximum inhibition zone (20 mm) against Staphylococcus aureus and followed by Klebsiella pneumoniae (17 mm). The MIC values of methanol extract were found to be 62.5µg/ml against Staphylococcus aureus, Proteus mirabilis and Bacillus subtilis. In the GC-MS analysis of phyto-compounds, the methanol extracts showed fourteen bioactive fractions and eleven compounds from acetone extract.

Conclusion: The methanol and acetone extracts of Coriandrum sativum seeds showed maximum inhibitory activity against the tested bacterial strains.

 

Keywords: Phytochemical screening, Antibacterial activity, Coriandrum sativum, Gas chromatography-Mass spectrometry

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Thangavel, A., S. K. Balakrishnan, V. ., and S. Duraisamy. “PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF CORIANDRUM SATIVUM (L) SEEDS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 153-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/7143.
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