ANTIOXIDANT, ANTIMICROBIAL, ANTIPROLIFERATIVE ACTIVITIES AND IN SILICO DRUG LIKENESS PREDICTION OF PURIFIED MHH COMPOUND, ISOLATED FROM MILLINGTONIA HORTENSIS LINN
Objective: To investigate the antioxidant, antimicrobial, antiproliferative activities and in silico drug likeness prediction of the MHH compound.
Methods: The antioxidant, antimicrobial and antiproliferative activities of the MHH compound have been evaluated by 1,1-Diphenyl-2-picryl hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays, agar well diffusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay respectively. The cytotoxicity activity and in silico drug likeness prediction of the MHH compound have been studied.
Results: The DPPH radical scavenging activity and the total antioxidant power of the MHH compound were found to be 92.48%Â±2.06 and 10.698Â±0.23 mM Fe (II)/g respectively. The antimicrobial activity of MHH compound against Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Staphylococcus aureus and Streptomyces griseus, Candida albicans, Aspargillus flavus and the zones of inhibition were found to be 16.83 mm, 19.83 mm, 15.23 mm, 16.79 mm, 13.57 mm, 19.65 mm, 18.66 mm and 14.79 mm respectively. The IC50 values of the MHH compound for HeLa and HCT-15 cell lines were found to be 371.92 Î¼g/ml and 112.06 Î¼g/ml respectively. The LC50 value of MHH compound was 218.765Âµg/ml and 152.78Âµg/ml which is considered as moderately cytotoxic and toxic respectively when incubated at 24 h and 48 h. The drug likeness and bioactivity scores of the MHH compound were found to be within the range and followed Lipinski's rule.
Conclusion: This study concludes that the MHH compound has significant biological activities and can be used as the therapeutic agent.
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