GENETIC POLYMORPHISM OF REVERSE TRANSCRIPTASE AND PROTEASE ASSOCIATED WITH THE ANTIRETROVIRAL (ARV) INEFFICIENCY IN PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 (HIV-2) IN ABIDJAN, CÔTE D’IVOIRE

  • Lohoues Essis Esmel C.
  • Trebissou Jonhson Noel D Pharmacodynamics Biochemical Laboratory, UFR Biosciences, Felix Houphouet Boigny University. PO Box, 582, Abidjan 22-Côte d’Ivoire
  • Dion Laine M.
  • Dechi Jean J.
  • N’din Jean L.
  • Camara Cisse M.

Abstract

Objectives: To analyse the genetic polymorphism of reverse transcriptase and protease genes in patients living with HIV-2, to search for possible mutations preventing the effectiveness of antiretroviral drugs (ARVs).

Methods: RNA Viral was extracted after lysis of the virus particles and purified on filter columns (Qiagen). After their extraction, DNA fragments were amplified by the method of reverse transcription/polymerase chain reaction (RT/PCR) on one hand and by the PCR method enchase on the other hand. The study of genetic polymorphisms in reverse transcriptase and protease was performed after sequencing of amplicons.

Results: A significant polymorphism was observed in positions involved in resistance to antiretrovirals. The most frequent was mutations M 46 I, M 36 I/V, V82I, L 89 I on protease and Y 181 I, Y 188 L, G 190A on reverse transcriptase.

Conclusion: Polymorphic mutations were observed in the genes of the protease and reverse transcriptase and could be involved in the HIV-2 resistance to antiretrovirals (ARVs). It would be desirable to do the quantification of DNA provirus of HIV-2 in the other for a durable therapeutic monitoring of patients having HIV-2.

Keywords: Human Immunodeficiency Virus (HIV-2), Reverse transcriptase, Protease, Abidjan, Côte d’Ivoire.

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Author Biography

Lohoues Essis Esmel C.
Abidjan

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C., L. E. E., T. J. Noel D, D. L. M., D. J. J., N. J. L., and C. C. M. “GENETIC POLYMORPHISM OF REVERSE TRANSCRIPTASE AND PROTEASE ASSOCIATED WITH THE ANTIRETROVIRAL (ARV) INEFFICIENCY IN PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 (HIV-2) IN ABIDJAN, CÔTE D’IVOIRE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 210-2, https://innovareacademics.in/journals/index.php/ijpps/article/view/7215.
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