CHEMOPREVENTIVE ROLE OF COMBINATION OF ETORICOXIB AND ATORVASTATIN ON COLON CANCER INDUCED BY 1, 2-DIMETHYL HYDRAZINE ON RATS
Objective: To investigate and compare the efficacy of atorvastatin, etoricoxib and combination of both drugs against colon carcinogenesis in male wistar rats.
Methods: Male wistar rats were divided into five groups. Group-1 served as normal control. Group-2 subcutaneously received 1,2-dimethylhydrazine (DMH) (20 mg/kg body weight(b. w.)) and served as DMH control. Groups-3, 4 and 5 were treated with DMH once in a week for 17 weeks. One week before the administration of DMH, group-3 and group-4 received etoricoxib (0.64 mg/kg per oral (p. o)), atorvastatin (2.5 mg/kg subcutaneously (s. c)) respectively and group-5 received both drugs and lasted until the end of the experiment. The effect of drugs on body weight gain, food and water intake, haematological parameter and histopathological view of the colon was observed in the entire group of animals.
Results: The experimental evidences showed that significant effect of the combined dose of etoricoxib and atorvastatin against DMH induced colon cancer by increasing the level of antioxidant enzymes. The combination was found to decrease the occurrence of multiple plaque lesions which may become the basis for its better chemo preventive action against the progression of colon carcinogenesis as compared to individual drugs. The histopathological study demonstrated that the combination treatment showed more positive effect then individual drug in prevention of colon carcinogenesis by reducing the inflammation, hyperplastic and dysplastic changes in colon crypt cells.
Conclusion: This study was concluded that the combination of atorvastatin and etori coxib may be potential chemo preventive agents against DMH-induced colon cancer and showed prominent positive effects as compared to individual drugs.
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