ANTIDIABETIC ACTIVITY OF CLERODENDRUM PHILIPPINUM SCHAUER LEAVES IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Objective: The present study has been undertaken to evaluate the antidiabetic activity of Clerodendrum philippinum Schauer leaves.
Methods: The fresh leaves were collected from Kuruan village of Jajpur district in the state of Odisha, India and extracted successively with n-hexane, methanol and water. The effect of extracts at the dose level of 400 mg/kg body weight was studied in normal, glucose loaded and streptozotocin-induced diabetic rats.
Results: The test extracts showed significant reduction of blood glucose level in normal, glucose loaded and streptozotocin-induced diabetic rats. Methanol extract demonstrated maximum blood glucose lowering potential as compared to other extracts.
Conclusion: The leaf of Clerodendrum philippinum Schauer is endowed with blood sugar lowering potential in both normal and diabetic rats.
2. Satapathy KB, Chand PK. Herbal cure of diabetes. Germany: Lap Lambert AG and Co; 2010. p. 217.
3. Shrivastava N, Patel T. Clerodendrum and heathcare: an overview. Med Aromatics Plant Sci Biotechnol 2007;1:209-23.
4. Anonymous. The Wealth of India, Raw Materials. Vol. 2. New Delhi: Council of Scientific and Industrial Research; 2001. p. 69.
5. Panthong A, Kanjanapothi D, Taylor WC. Ethnobotanical review of medicinal plants from Thai traditional books. Part 1. Plants with antiinflammatory, antiasthmatic and antihypertensive properties. J Ethnopharmacol 1986;18:213-28.
6. Chen PM, Yung LL, Hsiaq K, Chen CM, Yeh HM, Chuang MH. In vitro induction of differentiation in HL-60 leukemic cell line by Clerodendron fragrans. Am J Chin Med 1988;16:139-44.
7. Rao DM, Rao UVUB, Sudharshanam G. Ethno-medico-botanical studies from Rayalaseema region of southern eastern ghats, Andhra Pradesh, India. Ethnobotanical Leaflets 2006;10:198-207.
8. Sharma N, Verma HN. Effect of leaf extracts of Clerodendrum spp. on fruit rotting fungi. Fitoterapia 1991;62:517-8.
9. Lalitha KG, Sathish R, Gayathri R, Karthikeyan S, Kalaiselvi P, Muthuboopathi G, et al. Effect of leaf extracts of Clerodendrum spp. on fruit rotting fungi. Int J Pharm Res 2010;2:13-5.
10. Shrestha I, Joshi N. Medicinal plants of the Lele village of Lalitpur District, Nepal. Int J Pharmacogn 1993;31:130-4.
11. Barua AS, Chowdhury A, Adityachaudhury N. Chemical investigations of Clerodendron fragrans and Clerodendron siphonanthus. Indian J Chem 1989;28:878-9.
12. Hoang TH, Ha VH, Nguyen HK, Pyne SG. Flavonoids with antioxidant effects extracted recently from leaves of Clerodendron fragrans. Tap Chi Duoc Hoc 2000;3:10-2.
13. Gao LM, Wei XM, He YQ. Studies on chemical constituents in leafs of Clerodendron fragrans. Zhongguo Zhong Yao ZaZhi 2003;28:948-51.
14. Taoubi K, Fauvel MT, Gleye J, Fouraste I. Caffeic acid heteroside esters from verbenaceae. Bull Liaison Groupe Polyphenols 1992;16:174-7.
15. Singh P, Singh CL. Chemical investigations of Clerodendron fragrans. J Indian Chem Soc 1981;58:626-7.
16. Akihisa T, Matsubara Y, Ghosh P, Thakur S, Shimizu N, Tamura T, et al. The 24-alpha and 24-beta-epimers of 24-ethylcholesta-5,22-dien-3-beta-ol in two Clerodendrum species. Phytochemistry 1988;27:1169-72.
17. Akihisa T, Ghosh P, Thakur S, Oshikiri S, Tamura T, Matsumoto T. 24-beta-methylcholesta-5,22e,25-trien-3-beta-ol and 24-alpha-ethyl-5-alpha-cholest-22-e-en-3-beta-ol from Clerodendrum fragrans. Phytochemistry 1988;27:241-4.
18. Akihisa T, Matsubara Y, Ghosh P, Thakur S, Tamura T, Matsumoto T. Sterols of some Clerodendrum species (Verbenaceae): Occurrence of the 24-alpha-and 24-beta-epimers of 24-ethylsterolslacking a delta 25-bond. Steroids 1989;53:625-38.
19. Pari L, Satheesh MA. Antidiabetic activity of Boerhaavia diffusa L: effect on hepatic key enzymes in experimental diabetes. J Ethnopharmacol 2004;91:109â€“13.
20. Kokate CK, Purohit AP, Gokhale SB. Pathway to screen phytochemical nature of natural drugs. In: A text book of pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2007. p. 607-11.
21. Trease GE, Evans MC. Text book of Pharmacognosy. 12th ed. London: Balliere-Tindal; 1989. p. 343-83.
22. Adetuyi AO, Popoola AV. Extraction and dyes ability potential studies of the colourant in zanthoxylum zanthoxyloides plant on cotton fabric. J Sci Eng Technol 2001;8:3291-9.
23. Harbone JB, Baxter HH. Phytochemical Dictionary: A hand Book of Bioactive Compound from plants. Washington: Taylor and Francis; 1993. p. 237.
24. Jain S, Bhatia G, Barik R, Kumar P, Jain A, Dixit VK. Antidiabetic activity of Paspalum scrobiculatum Linn in alloxan induced diabetic rats. J Ethnopharmacol 2010;127:325â€“8.
25. Arulselvan P, Subramanian S. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic Î²cells in experimental diabetes in rats. Chem Biol Interact 2007;165:155â€“64.
26. Kar DM, Maharana L, Pattnaik S, Dash GK. Studies on hypoglycaemic activity of Solanum xanthocarpum Schrad & Wendl fruit extract in rats. J Ethnopharmacol 2006;108:251-6.
27. Senthilkumar P, Sudha S, Prakash S. Antidiabetic activity of aqueous extract of Padina boergesenii in streptozotocin-induced diabetic rats. Int J Pharm Pharm Sci 2014;6:418-22.
28. Rout SP, Kar DM, Mandal PK. Hypoglycaemic activity of aerial parts of Argemone mexicana L in experimental rat models. Int J Pharm Pharm Sci 2011;3 Suppl 5:533-40.
29. Ngueguim TF, Massa ZB, Kouamouo J, Tchuidjang A, Dzeufiet DPD, Kamtchouing P, et al. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. J Ethnopharmacol 2014;151:784-90.
30. Dheer R, Bhatnagar P. A study of the antidiabetic activity of Barleria prionitis Linn. Indian J Pharmacol 2010;42:70-3.
31. Gerspach AC, Steinert RE, Schonenberger L, Graber-Maier A, Beglinger C. The role of the gut sweet taste receptor in regulating GLP-1, PYY, and CCK release in humans. Am J Physiol: Endocrinol Metab 2011;301:317-25.
32. Mace OJ, Affleck J, Patel N, Kellett GL. Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2. J Physiol 2011;582:379-92.
33. Povi L, Batomayena B, HodÃ© TA, Kwashie E, Kodjo A, Messanvi G. Phytochemical screening, antioxidant and hypoglycemic activity of Coccoloba uvifera leaves and Waltheria indica roots extracts. Int J Pharm Pharm Sci 2015;7:279-83.
34. Tundis R, Loizzo MR, Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem 2010;10:315-31.
35. Kumar S, Narwal S, Kumar V, Prakash O. Î±-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacogn Rev 2011;5:19-29.
36. Sales PM, Souza PM, Simeoni LA, Silveira D. Î±-Amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci 2012;15:141-83.
37. Dou F, Xi M, Wang J, Tian X, Hong L, Tang H, et al. Alpha-glucosidase and alpha-amylase inhibitory activities of saponins from traditional Chinese medicines in the treatment of diabetes mellitus. Pharmazie 2013;68:300-4.
38. Mythili MD, Vyas R, Akila G, Gunasekaran S. Effect of streptozotocin on the ultrastructure of rat pancreatic islets. Microsc Res Tech 2004;63:274-81.