ANTIOXIDANT AND ANTIULCER POTENTIAL OF ETHANOLIC EXTRACT OF BARK OF MYRICA ESCULENTA IN PYLORIC LIGATION ULCER MODEL
Objective: To evaluate the antiulcer and antioxidant potential of ethanolic extract of bark of Myrica esculenta in pyloric ligated ulcer model in albino rats.
Methods: The rats were subjected to pyloric ligation ulcer model and the ethanolic extract of bark of Myrica esculenta were compared with the induced and standard drug Ranitidine. The activity of antioxidant parameters was analysed in the homogenate of stomachs.
Results: The levels of gastric volume, total acidity, free acidity, lipid peroxidation and glutathione (GSH) were reduced significantly and antioxidant, parameters like catalase, nitrite and myeloperoxidase (MPO) were increased significantly in the ethanloic extract of bark of Myrica esculenta.
Conclusion: The present study revealed that the ethanolic extract of bark of Myrica esculenta improved the anti ulcer and antioxidant potential.
2. Cozzarini W, Rath J, Bauer A, Gyorog I, Prenner M, Trianto T, et al. Mucosa protective therapy with long-term nonsteroidal antirheumatic drugs. Wien Med Wochenschr 2003;153:295-303.
3. Leontiadis GI, Sharma VK, Howden CW. Proton pump inhibitor treatment for acute peptic ulcer bleeding. Cochrane Database of Systematic Rev 2006;1:CD002094.
4. Nadkarni KM. Indian Materia Medica I. Bombay: Popular Book Depot; 1954. p. 828-9.
5. Malterud KE, Diep OH, Sund RB. C-Methylated dihydrochalcones from Myrica gale L: Effects as antioxidants and as scavengers of 1,1-diphenyl-2-picryl hydrazyl. Annu Rev Pharmacol Toxicol 1996;78:111â€“6.
6. Kokate CK, Purohit AP, Gokhale SB. Text book of Pharmacognosy. 34th ed. Pune: Nirali prakashan; 2006. p. 337.
7. Shay M, Komarov SA, Fels D, Meranze D, Gruenstein H, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 1945;5:43-61.
8. Rao CM, Ramesh KV, Biary KL, Kulkarni DR. Zinc complexes of NSAIDs abolish gastric ulceration propensity of parent drugs. Indian Drugs 1990;28:64-7.
9. Parmar NS, Desai JK. A review of the current methodology for the evaluation of gastric and duodenal anti-ulcer agents. Indian J Pharmacol 1993;25:120-35.
10. Susan G, Sathimoorthy A, Sathimoorthy SS. Effect of Î±-tocopherol on gastric ulcers induced by pylorus ligation in rats. Ind J Pharmacol 1990;31:431-3.
11. Mukherjee KL. Medical Laboratory Technology. 3rd ed. New Delhi: Tata. Mc Graw-Hill; 1989. p. 1086-8.
12. Naveen T, Sangeeta P, Anurag K, Kanwaljit Chopra. Hesperidine, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetra chloride in rat liver and kidney. BMC Pharmacol Toxicol 2005;471:221-30.
13. Niehaus WG Jr, Samuelsson B. Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968;6:126-30.
14. Jollow D, Mitchell L, Zampaglione N, Gillete J. Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3, 4-bromobenzenoxide as the hepatotoxic intermediate. Pharmacol 1974;11:151-69.
15. Aebi H. Oxidoreductases acting on groups other than CHOH: Catalase. Methods Enzymol 1984;105:121-5.
16. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Anal Biochem 1982;126:131-8.
17. Mullen KM, Bruce Smith ND. Myeloperoxidase activity as a quantitative assessment of neutrofil infiltration into ischemic myocardium. J Pharmacol Methods 1995;14:157-67.
18. Mandal S, Das DN, De K, Ray K, Roy G, Chaudhuri SB, et al. Ocimum sanctum Linnâ€“A study on gastric ulceration and gastric secretion in rats. Ind J Physiol Pharmacol 1993;37:91-2.
19. Das D, Bandyopadhyay D, Bhattacharjee M, Banerjee RK. Hydroxyl radical is the major causative factor in stress-induced gastric ulceration. Free Radic Biol Med 1997;23:8-18.
20. John M. C Gutteridge and Barry Halliwell. Free radical and antioxidant in the year 2000: A historical look to the future. Ann N Y Acad Sci 2000;899:136-47.
21. Lau D, Baldus S. Myeloperoxidase and its contributory role in inflammatory vascular disease. Pharmacol Ther 2006;111:16-26.