DRAFT GENOME SEQUENCE OF HUMAN HERPES VIRUS-4 VRF_EBV_01, AN EPSTEIN BARR VIRUS OBTAINED FROM A PEDIATRIC POST TRANSPLANT LYMPHOPROLIFERATIVE DISORDER (PTLD) PATIENT

  • J Malathi Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
  • Mk Janani Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
  • N Murugan Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
  • Madhavan Hn Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006

Abstract

Objectives: To decode the sequence of Epstein-barr virus (EBV) genome isolated from a pediatric patient with post transplant lymphoproliferative disorder (PTLD).

Methods: EBV culture harvested from the blood sample of a 4-year-old patient with post liver transplant lymphoproliferative disorder prior to treatment was subjected to whole genome sequencing using Illumina platform. Generated data were subjected to various quality analysis and the filtered sequences were submitted to NCBI and published under accession number KM269735-KM269744.

Results: Annotation results of VRF_ EBV01 genome using Prokka tool and manual blast search infers 48 hypothetical proteins, each 3 genes coding for Epstein-Barr nuclear antigen 3A and 3B and each one gene coding for Epstein-Barr nuclear antigens 1, 4, 4B and DNA primase UL70 protein. Single copy of genes coding for each variant of Tegument protein BSRF1, BLRF2, BGLF2 mir-BART 1,2,3,5,7,12,15,17,20 and two copies of gene coding for primary envelopment factor BFRF1 gene was also found.

Conclusion: To date, only eight EBV genome sequences have been reported worldwide and there is no genome sequence reported from India. This study is the first of its kind to report on EBV genome from a post–transplant lymphoproliferative disorder to the scientific community for the welfare of research against EBV diagnostic markers and drug discovery.

 

Keywords: Herpes virus, Genome sequence, Epstein - Barr virus, lymphoproliferative disorder

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Author Biographies

J Malathi, Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006

Associate Professor, 

L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai-600 006

Mk Janani, Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
Junior Scientist
N Murugan, Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
Senior Research Fellow
Madhavan Hn, Dept of Microbiology, L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai 600006
Director, Research

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How to Cite
Malathi, J., M. Janani, N. Murugan, and M. Hn. “DRAFT GENOME SEQUENCE OF HUMAN HERPES VIRUS-4 VRF_EBV_01, AN EPSTEIN BARR VIRUS OBTAINED FROM A PEDIATRIC POST TRANSPLANT LYMPHOPROLIFERATIVE DISORDER (PTLD) PATIENT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 513-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/7462.
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Short Communication(s)