IN VITRO CYTOTOXICITY AND FREE RADICAL SCAVENGING POTENTIAL OF ETHYL PYRUVATE DERIVED COPPER COMPLEXES OF THIOSEMICARBAZONE

  • Kittur R. Asha Davangere University
  • Heggodu G. Rohitkumar Davangere University
  • Kalagouda B. Gudasi Karnatak Universit
  • Gopal M. Advi Rao Department of Biochemistry, Davangere University, Davangere 577002

Abstract

Objective: The present study was aimed to evaluate the cytotoxicity and antioxidant properties of copper complexes of thiosemicarbazone (TSC-Cu) using different in vitro models.

Methods: In vitro MTT assay was carried out against the HL-60 cell line to check the cytotoxic potency of TSC-Cu. In vitro radical scavenging activity of TSC-Cu was evaluated by checking its role in scavenging DPPH, hydroxyl radical and lipid peroxidation.

Results: Among the test molecules studied, TSC-Cu with choride side chain (TSC-Cu-Cl) showed dose and time dependent increased cytotoxic activity on HL-60 cell line compared to TSC-Cu with hydroxyl side chain (TSC-Cu-OH). Further, TSC-Cu-Cl exhibited promising radical scavenging activities against hydroxyl radical, 2,2-diphenyl, 2-picryl hydrazyl (DPPH), and also effectively inhibited lipid peroxidation.

Conclusion: The results of the present investigation confirm that the TSC-Cu-Cl complex can be considered as a novel metal complex with potent anti-oxidant and cytotoxic ability.

 

Keywords: Thiosemicarbazone, Cytotoxicity, Antioxidant, Leukemia, Anticancer drug

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How to Cite
Asha, K. R., H. G. Rohitkumar, K. B. Gudasi, and G. M. A. Rao. “IN VITRO CYTOTOXICITY AND FREE RADICAL SCAVENGING POTENTIAL OF ETHYL PYRUVATE DERIVED COPPER COMPLEXES OF THIOSEMICARBAZONE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, Aug. 2015, pp. 128-32, https://innovareacademics.in/journals/index.php/ijpps/article/view/7515.