• Siddanakoppalu N. Pramod Laboratory for Immunomodulation and Inflammation Biology, Department of Studies in Biochemistry, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga 577203, Karnataka, India
  • Vellingiri Vigneshwaran Laboratory for Immunomodulation and Inflammation Biology, Department of Studies in Biochemistry, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga 577203, Karnataka, India
  • Yeldur P. Venkatesh Department of Biochemistry and Nutrition, Central Food Technological Research Institute (CFTRI), Mysore 570020, Karnataka, India


Objective: Potato (Solanum tuberosum) and garlic (Allium sativum) are an important ubiquitously consumed dietary components and known to contain lectins, with potent and diverse biological functions. Present study aims to evaluate the mitogenicity and immunomodulatory potential of these lectins on human and murine lymphocytes.

Methods: The lectin activity of raw and heat processed extracts along with purified proteins were confirmed by heamagglutination and glycoprotein binding assays. MTT assay employed to evaluate the mitogenic and cytotoxic potential. Nitric oxide and NBT assays were performed for NO and ROS induction from lymphocytes and macrophages respectively.

Results: Raw and heat processed extracts of potato and garlic induced heamagglutination and demonstrated binding affinity to glycoproteins. Potato lectin was identified as pan agglutinin whereas garlic lectins, were blood group specific with differential glycan recognition. Garlic extracts and lectins (ASAs) exhibited a strong mitogenicity towards human and murine lymphocytes. The proliferation index for ASA I (70-75%) and ASA II (35-40%) are comparable to those of the reference mitogens. However, Potato extracts and lectin (STA) was non-mitogenic and found cytotoxic for both human and murine lymphocytes. Raw garlic extract showed a significant increase in ROS generation. Both potato and garlic lectins failed to induce ROS from macrophages and nitric oxide (NO) from human PBLs.

Conclusions: Both potato and garlic lectins were capable of interacting with cell surface glycoproteins and it is evident that the garlic lectins have stimulatory effect whereas the potato lectin has an inhibitory effect on lymphocyte proliferation. There by, study indicates that garlic lectins are mitogenic and co-mitogenic, whereas potato lectin is non-mitogenic or anti-mitogenic in nature.


Keywords: Garlic, Agglutinin, Lectin, Lymphocytes, Mitogen, Potato


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1. Sharon N, Lis H. History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology 2004;14:53-62.
2. Wu AM, Lisowska E, Duk M, Yang Z. Lectins as tools in glycoconjugate research. Glycoconj J 2009;26:899-913.
3. Shoran N. Lectins: past, present and future. Biochem Soc Trans 2008;36:1457-60.
4. Ashraf MT, Khan RH. Mitogenic lectins. Med Sci Monit 2003;9:265-9.
5. Kilpatrick DC. Mechanisms and assessment of lectin mediated mitogenesis. Mol Biotechnol 1999;11:55-65.
6. Yamaguchi K, Uechi M, Katakura Y, Oda T, Ishiguro M. Mitogenic properties of pokeweed lectin D-isoforms on pheripheral blood lymphocytes: nonmitogen PL-D1 and mitogen PL-D2. Biosci Biotechnol Biochem 2004;68:1591-3.
7. Shanmugham LN, Castellani ML, Salini V, Falasca K, Vecchiet J, Conto P. Petrarca C. Relevance of plant lectins in human cell biology and immunology. Riv Biol 2006;99:227-49.
8. Cunha EV, Costa JJ, Rossi RO, Silva AW, Passos JR, Portela AM, et al. Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicules. Braz J Med Biol 2013;46:245-52.
9. Velasquez EV, Rios M, Ortiz ME, Lizama C, Nunez E, Abramovich D, et al. Concanavalin-A induces granulosa cell death and inhibits FSH-mediated follicular growth and ovarian maturation in female rats. Endocrinology 2013;154:1885-96.
10. Kaur N, Singh J, Kamboja SS, Agrewala JN, Kaur M. Two novel lectins from Parkia biglandulosa and Parkia roxburghii: isolation, physicochemical characterization, mitogenicity and anti-proliferative activity. Protein Pept Lett 2005;12:585-95.
11. de Melo CM, de Castro MC, de Oliveira AP, Gomes FO, Pereira VR, Coelho LC, et al. Immunomodulatory response of Cramoll 14 lectin on experimental lymphocytes. Phytother Res 2010;24:1631-6.
12. Pramod SN, Venkatesh YP. Utility of pentose colorimetric assay for the purification of potato lectin, an arabinose-rich glycoprotein. Glycoconj J 2006;23:481-8.
13. Clement F, Pramod SN, Venkatesh YP. Identity of the immunomodulatory proteins from garlic (Allium sativum) with the major garlic lectins or agglutinins. Int Immunopharmacol 2010;10:316-24.
14. Morihara N, Sumioka I, Ide N, Moriguchi T, Uda N, Kyo E. Aged garlic extract maintains cardiovascular homeostasis in mice and rats. J Nutr 2006;136:777-81.
15. Colić M, Vucević D, Kilibarda V, Radicević N, Savić M. Modulatory effects of garlic extracts on proliferation of T-lymphocytes in vitro stimulated with concanavalin A. Phytomedicine 2002;9:117-24.
16. Pramod SN, Venkatesh YP, Mahesh PA. Potato lectin activates basophil and mast cells of atopic subjects by its interaction with core chitobiose of cell-bound non-specific immuno-globulin E. Clin Exp Immunol 2007;148:391-401.
17. Dong Q, Sugiura T, Toyohira Y. Yanagihara N, Karasaki Y. Stimulation of IFN- production by garlic lectin in mouse spleen cells: involment of IL-12 via activation of p38 MAPK and ERK in macrophages. Phytomedicine 2011;18:309-16.
18. Pierini D, Bryan NS. Nitric oxide availability as a marker of oxidative stress. Methods Mol Biol 2015;1208:63-71.
19. Jothiramajayam M, Sinha S, Ghosh M, Nag A, Jana A, Mukherjee A. Sodium fluoride promotes apoptosis by generation of reactive oxygen species in human lymphocytes. J Toxicol Environ Health A 2014;77:1269-80.
20. Ravinder S, Sreedhar S, Jonathan MR, Barry JC. Peanut lectin stimulates proliferation of colon cancer cells by interaction with glycosylated CD44v6 isoforms and consequential activation of c-Met and MAPK: functional implications for disease-associated glycosylation changes. Glycobiology 2006;16:594–601.
21. Singh RS, Walia AK. Microbial lectins and their prospective mitogenic potential. Crit Rev Microbiol 2014;40:329-47.
22. Wimer BM, Mann PL. Mitogen information summaries. Cancer Biother Radiopharm 2002;17:569-97.
23. McCurrach PM, Kilpatrick DC. Datura lectin is both an anti-mitogen and co-mitogen acting synergistically with phorbol ester. Scand J Immunol 1988;27:31-4.
24. Clement F, Venkatesh YP. Dietary garlic (Allium sativum) lectins ASA I and ASA II are highly stable and immunogenic. Int Immunopharmacol 2010;10;1161-9.
25. Lavelle EC, Grant G, Pfuller U, O’Hagan DT. Immunological implications of the use of plant lectins for drug and vaccine targeting to the gastrointestinal tract. J Drug Target 2004;12:89-95.
26. Pusztai A, Bardocz S, Ewen SW. Uses of plant lectins in bioscience and biomedicine. Front Biosci 2008;13:1130-40.
27. Sharon N. Lectins: carbohydrate-specific reagents and biological recognition molecules. J Biol Chem 2007;282:2753-64.
28. Ashraf MT, Khan RH. Mitogenic lectins. Med Sci Monit 2003;9:265-9.
29. DammI, Mikkat U, Kirchhoff F, Bockmann S, Joas L. inhibitory effect of the lectin wheat germ agglutinin on the binding of 1251-CCK-*s to the CCK-A and B receptors of AR42J cells. Pancreas 2004;28:31-7.
30. Greene WC, Waldmann TA. Inhibition of human lymphocyte proliferation by the nonmitogenic lectin wheat germ agglutinin. J Immunol 1980;124:2979-87.
31. Kilpatrick DC, Graham C, Urbaniak SJ. Inhibition of human lymphocyte transformation by tomato lectin. Scand J Immunol 1986;24:11-9.
32. Reed JC, Sabath DE, Hoover RG, Prystowsky MB. Recombinant interleukin 2 regulates levels of c-myc mRNA in a cloned murine T lymphocyte. Mol Cell Biol 1985;5:3361-8.
33. Matsubara K, Higaki T, Matsubara Y, Nawa A. Nitric oxide and reactive oxygen species in the pathogenesis of preeclampsia. Int J Mol Sci 2015;16:4600-14.
34. Lei Y, Wang K, Deng L, Chen Y, Nice EC, Huang C. Redox regulation of inflammation: old elements, a new story. Med Res Rev 2015;35:306-40.
35. Forstermann U, Sessa WC. Nitric oxide synthases: Regulation and function. Eur Heart J 2012;33:829–37.
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How to Cite
Pramod, S. N., V. Vigneshwaran, and Y. P. Venkatesh. “IMMUNOMODULATORY EFFECTS OF HEMAGGLUTINATING LECTINS FROM POTATO (SOLANUM TUBEROSUM) AND GARLIC (ALLIUM SATIVUM) ON HUMAN AND MURINE LYMPHOCYTES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, Sept. 2015, pp. 147-53,