PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF L-AMINO ACID OXIDASE FROM WESTERN REGION INDIAN COBRA (NAJA NAJA) VENOM

  • Neema K. N. Department of Biotechnology, Sri Jayachamarajendra College of Engineering, Mysore 570006, Karnataka, India, JSS Research foundation, JSS Technical Institutions campus, Mysore 570006
  • Vivek H. K Department of Biotechnology, Sri Jayachamarajendra College of Engineering, Mysore-570006, Karnataka, India
  • Kumar J. R. Post Graduate Department of Biochemistry, JSS College of Arts, Commerce and Science, Mysore 570025, Karnataka, India
  • B. S. Priya Department of studies in Chemistry, University of Mysore, Manasagangothri, Mysore 570006, Karnataka, India
  • S. Nanjunda Swamy Department of Biotechnology, Sri Jayachamarajendra College of Engineering, Mysore 570006, Karnataka, India

Abstract

Objective: Purification and biochemical characterization of LAAO from western region Indian Cobra (Naja naja) venom.

Methods: LAAO was purified from Indian cobra (Naja naja) venom using sequential chromatography on Sephadex G-75 gel filtration followed by Ion exchange on CM-Sephadex C-25 column. Biochemical characterization viz., pH, Temperature, Km and Vmax were determined. Molecular weight of LAAO was determined by electrophoresis. Inhibition of LAAO from cold water extracts of Curcuma zedoria, Curcuma ceasia, Curcuma aromatic, Curcuma longa, Curcuma amada, Cucumis sativus and Benincasa hispida was done.

Results: Purified LAAO showed the single band on non reducing SDS-PAGE with approximate molecular weight of 65 kDa. Further biochemical characterization revealed that, LAAO from Naja naja (western region) has an optimum pH of 7.0 and is stable at room Temperature upto 37 °C and showed an optimum enzyme concentration of 4µg/ml and Km 134.1µM and Vmax is 21.87 U/min. Cold water extract of Curcuma ceasia, Curcuma aromatic and Benincasa hispida showed comparatively significant inhibition of LAAO.

Conclusion: LAAO has promising therapeutic prospects because of its effects on various biological functions. Variation in snake species and their geographical distribution also contributes to the venom properties such as composition, toxicity level, pharmacological and biological activities. The significant difference in protein profiling leads to substantial lethality among different geographical regions. Thus in this study Indian Cobra (Naja naja) venom from the western region of India was subjected to purification of LAAO and biochemical characterization.

 

Keywords: Indian Cobra (Naja naja), Venom, L amino acid oxidase-LAAO

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How to Cite
N., N. K., V. H. K, K. J. R., B. S. Priya, and S. N. Swamy. “PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF L-AMINO ACID OXIDASE FROM WESTERN REGION INDIAN COBRA (NAJA NAJA) VENOM”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, Sept. 2015, pp. 167-71, https://innovareacademics.in/journals/index.php/ijpps/article/view/7665.