EVALUATION OF IMMUNOMODULATORY ACTIVITY OF PETROLEUM ETHER EXTRACT OF SEEDS OF PITHECELLOBIUM DULCE IN WISTAR RATS

  • Nabin Wagle Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
  • S Nagarjuna Center of Pharmaceutical Research (CPR)
  • A. Sudheer Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
  • Chitrala Roopesh Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
  • Hari Prasad Sapkota Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
  • Nim Bahadur Dangi Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
  • Raviraja Pradhan Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)

Abstract

Objective: The present study was planned to assess the immunomodulatory action of petroleum ether extracts of seeds of Pithecellobium dulce (PEPD) in experimental models of immunity.

Methods: Metronidazole, pyrogallol and ethanol models were used for suppression of the immune system in Wistar rats. Humoral immunity was analyzed by haemmagglutination assay, whereas, cellular immunity was carried out by paw edema and carbon clearance assay. PEPD (400 mg/kg, p. o.) was selected by acute toxicity study. Levamisole (50 mg/kg, p. o.) was used as standard.

Results: Metronidazole, pyrogallol and ethanol significantly decreased the humoral and cellular immunity, whereas, PEPD and levamisole significantly increased the circulating antibody titer in the indirect haemagglunation test. PEPD and levamisole also produced significant increases in paw edema and increase in the phagocytic index in the carbon clearance assay. Animals treated with metronidazole, pyrogallol and ethanol decreased in peripheral blood RBCs and monocytes whereas, PEPD and levamisole treated groups significantly increased in peripheral blood RBCs and monocytes.

Conclusion: Our data showed that PEPD possesses potential for augmenting immune activity by cellular and humoral mediated mechanisms and also significantly restores the biochemical and histopathological parameters. The present study concluded that PEPD may have therapeutic and prophylactic value as immunostimulants.

 

Keywords: Pithecellobium dulce, Immunomodulation, Haemagglunation, Paw edema, Phagocytic response

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Author Biographies

Nabin Wagle, Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
Pharmacology
A. Sudheer, Division of Pharmacology, Center of Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research (RIPER), Saigram Krishnamreddy Palli Cross, Chiyyedu (PO) Anantapuramu 5155721 (A. P.)
Pharmacology

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How to Cite
Wagle, N., S. Nagarjuna, A. Sudheer, C. Roopesh, H. P. Sapkota, N. B. Dangi, and R. Pradhan. “EVALUATION OF IMMUNOMODULATORY ACTIVITY OF PETROLEUM ETHER EXTRACT OF SEEDS OF PITHECELLOBIUM DULCE IN WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 471-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/7729.
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