CAMPTOTHECIN LOADED POLY (METHACYCLIC ACID-CO-METHYL-METHYACRYLATE) NANOPARTICLES: FABRICATION, CHARACTERIZATION AND CYTOTOXICITY STUDIES
Objectives: The present investigation was aimed to fabricate Camptothecin loaded Poly (Methacyclic acid-co-methyl-methyacrylate) nanoparticles to characterize the prepared nanoparticles and to evaluate the cytotoxicity of prepared Camptothecin.
Methods: Camptothecin loaded polymeric nanoparticles were prepared by nanoprecipitation method by sonication approach. The prepared nanoparticles were evaluated for particle size, particle size uniformity, surface area, zeta potential, surface morphology, encapsulation efficacy, drug loading and in-vitro drug release. To evaluate the potential anticancer efficacy of nanoparticulate system, in-vitro cytotoxicity studies on human colon cancer cell line (HT-29) were carried out using MTT assay.
Results: Camptothecin loaded polymeric nanoparticles were successfully prepared by nano precipitation method using sonication approach. Nanoparticles prepared using sonication method were with an average particle size 100 to 200 nm, particle size uniformity found to be<0.3 and zeta potential>20mV. Prepared Camptothecin loaded polymeric nanoparticles were spherical in shape and showed excellent encapsulation efficiency and drug loading. The release profile was found to be pH dependent. It was observed that polymer coated Camptothecin nanoparticles gave no release in simulated gastric fluid, negligible release in simulated intestinal fluid and maximum release in colonic environment. The drug release followed Hixson Crowell cube root law model. Prepared Camptothecin loaded polymeric nano formulations displayed enhanced cytotoxicity against HT-29 cells in comparison with pure Camptothecin.
Conclusion: In summary, Camptothecin loaded Poly (Methacyclic acid-co-methyl-methyacrylate) nanoparticles may be considered as an attractive and promising formulation. Thus, the results indicate the potential for in-vivo studies for the developed pH sensitive nanoparticles of Camptothecin to establish their clinical application.
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