EVALUATION OF PHYTOCONSTITUENTS, IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF AGARICUS BISPORUS
Objective: The present study was aimed to investigate the phytochemicals, antimicrobial and antioxidant potential of wild edible mushroom (Agaricus bisporus).
Methods: Initially, phytochemical screening was carried out to identify the major phytoconstituents present in A. bisporus. Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis were executed to study the phytochemical profile of A. bisporus methanolic extract. On the other hand, in vitro antioxidant activity was evaluated by reducing power assay and H2O2 scavenging assay. Also, Antibacterial activity was assessed by the agar-well diffusion method.
Results: The total phenol content was found to be 5.8 mg GAE/g in aqueous extract and 7.4 mg GAE/g in the methanolic extract of A. bisporus. Antioxidant assays clearly indicate that the methanolic extract of A. bisporus displays highest H2O2 scavenging activity than aqueous extract. The methanolic extract of A. bisporus gave a higher reducing power than the aqueous extract depicts its antioxidant potential. Similarly, methanolic extract of A. bisporus gives excellent antimicrobial activity against tested human pathogens whereas aqueous extract showed comparatively less action. A Higher level of the zone of inhibition was measured against P. aureginosa and S. aureus whereas E. coli and B. subtilus displayed a moderate level of inhibitory action.
Conclusion: The overall results suggest that methanolic extract of A. bisporus has shown stupendous antioxidant and antimicrobial potential. This study can be useful to develop commercially viable natural drugs for biomedical applications.
Keywords: Agaricus bisporus, GC-MS analysis, Antioxidant, Human pathogens, Phenolic compounds.
2. Khlebnikov AI, Schepetkin IA, Domina NG, Kirpotina LN, Quinn MT. Improved quantitative structureâ€“activity relationship models to predict the antioxidant activity of flavonoids in chemical, enzymatic, and cellular systems. Bioorg Med Chem 2007;15:1749-70.
3. Ahmad P, Sarwat M, Sharma S. Reactive oxygen species, antioxidants and signalling in plants. J Plant Biol 2008;51:167-73.
4. Sun J, Chu YF, Wu X, Liu RH. Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem 2002;50:7449â€“54.
5. Yaltirak T, Aslim B, Ozturk S, Alli H. Antimicrobial and antioxidant activities of Russula delica. Fr. Food Chem Toxicol 2009;47:2052â€“6.
6. Agrahar-Murugkar D, Subbulakshmi G. Nutritional value of edible wild mushrooms collected from the khasi hills of meghalaya. Food Chem 2005;89:599âˆ’603.
7. Lindequist U, Niedermeyer THJ, Julich WD. The pharmacological potential of mushrooms. J Evidence-Based Complementary Altern Med 2005;2:285â€“99.
8. Jose GS, Radhamany PM. Identification and determination of antioxidant constituents of bioluminescent mushroom. Asian Pac J Trop Biomed 2012;2:S386-S391.
9. Adebayo EA, Oloke JK, Ayandele AA, Adegunlola CO. Phytochemical, antioxidant and antimicrobial assay of mushroom metabolite from Pleurotus pulmonariusâ€“LAU 09 (JF736658). J Microbiol Biotech Res 2012;2:366-74.
10. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307â€“15.
11. Ruch RJ, Cheng SJ, Klauning JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003â€“8.
12. Arya C, Arya A. Effect of acid hydrolysis of substrate on yield of oyster mushroom Pleurotus sajor-caju (Fr.) Singer. Mushroom Res 2003;12:35-8.
13. Janakiraman N, Sathish SS, Johnson M. UV-VIS and FTIR spectroscopic studies on Peristrophe bicalyculata (Retz.) Nees. Asian J Pharm Clin Res 2011;4:125-29.
14. Brinkhaus B, Lindner M, Schuppan D, Hahn EG. Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine 2000;7:427-48.
15. Ferreira ICFR, Vaz JA, Vasconcelos MH, Martins A. Compounds from wild mushrooms with antitumor potential. Anti-Cancer Agents Med Chem 2010;10:424â€“36.
16. Liu C, Wang C, Xu Z, Wang Y. Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis miller irrigated with sea water. Process Biochem 2007;42:961â€“70.
17. Reis FS, Martins A, Barros L, Ferreira ICFR. Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples. Food Chem Toxicol 2012;50:1201â€“7.
18. Liu J, Jia L, Kan J, Jin C. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food Chem Toxicol 2013;51:310-6.
19. Li JW, Ding SD, Ding XL. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochem 2005;40:3607â€“13.
20. Shimamura, Zhao T, Hu WH, Qing Z. Mechanism of action and potential for use of tea catechin as an antiinfective agent. Anti-Infect Agents Med Chem 2007;6:57-62.
21. Baise HP, Walker TS, Stermitz FR, Hufbauerand RS, Vivanco JM. Enantiometric dependent phytotoxic and antimicrobial activity of (Â±)â€“catechin; a rhizosecreted racemic mixture from Centaurea maculosa (spotted knapweed). Plant Physiol 2002;128:1173-7.