EVALUATION OF IN VITRO ANTIOXIDANT POTENTIAL OF SPENT CHILLI AND SPENT CUMIN OBTAINED AFTER OLEORESIN EXTRACTION
Objective: Chilli spent residue (CHSR) and Cumin spent residue (CSR) obtained after oleoresin extraction is known to be rich in polyphenols, however, the very limited commercial application is known except for its use as veterinary feeds. Considering that a huge residue is left over by oleoresin spice industry, application-oriented utilization of spent residue of chilli and cumin is warranted. In these lines, we in this study evaluate the antioxidant potential of Chilli spent residue (CHSR) and Cumin spent residue (CSR) obtained after oleoresin extraction by DPPH method.
Methods: In vitro radical scavenging activity of CHSR and CSR obtained after oleoresin extraction was evaluated by checking its role in scavenging DPPH.
Results: The spent chilli extract exhibited higher DPPH scavenging activity when compared to the spent cumin and the IC50 values of spent chilli; spent cumin and ascorbic acid were found to be 186.23Â±1.05 Âµg/ml, 284Â±1.03 Âµg/ml and 33.21Â±1.04, respectively. As CHSR and CSR obtained after oleoresin extraction is known to be rich in polyphenols, these might be responsible for potent and significant antioxidant activity observed.
Conclusion: This study shows that by-products obtained/generated in oleoresin industry can be utilized as value added product. Future work will be interesting to know the chemical composition and better understand the mechanism of action of the antioxidants present in the extract for development as a drug for therapeutic application.
2. Gossell-Williams M, Simon OR, West ME. The past and present use of plants for medicines. West Indian Med J 2006;55:217â€“8.
3. Auudy B, Ferreira F, Blasina L, Lafon F, Arredondo F, Dajas R, et al. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol 2003;84:131-8.
4. Shrestha S, Subaramaihha SR, Subbaiah SG, Eshwarappa RS, Lakkappa DB. Evaluating the antimicrobial activity of methanolic extract of rhus succedanea leaf gall. Bioimpacts 2013;3:195-8.
5. Shrestha S, Kaushik VS, Eshwarappa RS, Subaramaihha SR, Ramanna LM, Lakkappa DB. Pharmacognostic studies of insect gall of Quercus infectoria Olivier (Fagaceae). Asian Pac J Trop Biomed 2014;4:35-9.
6. Blois MS. Antioxidants determination by the use of a stable free radical. Nature 1958;181:1199â€“200.
7. Bracco U, Loliger J, Viret J. Production and use of natural antioxidant. J Am Oil Chem Soc 1981;58:686-90.
8. Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohani DP. Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemicals 2003;63:97â€“104.
9. Sowbhagya HB, Soumya C, Indrani D, Srinivas P. Physio-chemical characteristics of chilli spent residue and its effect on the rheological, microstructural and nutritional qualities of bread. J Food Sci Technol 2015;52:7218-26.
10. LeÃ³n-GonzÃ¡lez AJ, Auger C, Schini-Kerth VB. Pro-oxidant activity of polyphenols and its implication on cancer chemoprevention and chemotherapy. Biochem Pharmacol 2015;98:371-80.