• Hemlata Bhosale DST-Fist Sponsored School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, M. S., India 431606
  • Priti Bismile Swami Ramanand Teerth Marathwada University, Nanded, M. S., India 431606
  • Tukaram Kadam Swami Ramanand Teerth Marathwada University, Nanded, M. S., India 431606
  • Uzma Shaheen Swami Ramanand Teerth Marathwada University, Nanded, M. S., India 431606


Objective: To screen the actinobacterial strains associated with root rhizosphere of Curcuma longa for antioxidant, enzyme inhibitory and antifungal potential and to identify the potent strain TRA-1.

Methods: The strains were isolated on starch casein agar (SCA). The antioxidant, enzyme inhibitory and antifungal activities were studied using standard methods. Identification of active strain TRA-1 was carried out by studying morphological and cultural characteristics and by employing 16S rRNA sequencing.

Results: The detected phenolic content and total antioxidant activities were in the range of 1µg/ml to 80 µg/ml and 0.198 to 1.088 each. Diphenyl picrylhydrazyl (DPPH) and hydroxyl radicals scavenging activity and capacity to chelate metal ion and reduce ferric iron were found in 11, 14, 6 and 12 strains respectively. The activities of α-amylase and α-glucosidase were inhibited by crude extracts of twelve and three isolates and the extracts of ten isolates inhibited lipase activity. Nine isolates inhibited the growth of one or more test fungal pathogens. All test pathogens were sensitive to TRA-1. The active strain TRA-1 was identified as Streptomyces albus. The active metabolite of TRA-1 showed 41.43% DPPH scavenging activity, 54.48% and 34.46% respective inhibition in α-amylase and lipase activity and was highly active against C. neoformans (35 mm) followed by A. niger (18 mm).

Conclusion: The results of the study showed remarkable antioxidant, enzyme inhibitory and antifungal potential of isolated strains. The potent strain Streptomyces albus TRA-1 can be used as a promising source for isolation of pharmaceutically important bioactive compounds.

Keywords: Antioxidant, Inhibition, Enzyme, Antifungal, Streptomyces albus.


Download data is not yet available.


1. Demain AL, Sanchez S. Microbial drug discovery: 80 y of progress. J Antibiot 2009;62:5-16.
2. Blunt JW, Princep MR. Marine natural products. Nat Prod Rep 2006;23:26-78.
3. Ouédraogo M, Konaté K, Zerbo P, Barro N, Laya L. Phytochemical analysis and in vitro antifungal profile of bioactive fractions from Sterculia setigera (Sterculiaceae). Curr Res J Biol Sci 2013;5:75-80.
4. Berdy J. Bioactive microbial metabolites. J Antibiotechnol (Tokyo) 2005;58:1-26.
5. Sharma SK, Gupta VK. In vitro antioxidant studies of Ficus resimosa linn root. Pharmacogn Mag 2008;13:70-4.
6. Bull AT, Ward AC, Goodfellow M. Search and discovery strategies for biotechnology: the paradigm shift. Microbiol Mol Biol 2000;64:573-606.
7. Watve MG, Tickoo R, Jog MM, Bhole BD. How many antibiotics are produced by the genus streptomyces? Arch Microbiol 2001;176:386-90.
8. Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89–96.
9. Kuster E, Williams ST. Selective media for the isolation of streptomycetes. Nature 1964;202:928-9.
10. Zhao GR, Xiang ZJ, Ye TX, Yaun JY, Guo XZ. Antioxidant activities of saliva miltiorrhiza and panax notoginseng. Food Chem 2006;99:767-74.
11. Singleton VL, Orthofehr R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol 1999;299:152-78.
12. Chang LW, Yen WJ, Huang SC, Duh PD. Antioxidant activity of sesame coat. Food Chem 2002;78:347-54.
13. Elizabeth K, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
14. Oyaizu M. Studies on the product of browning reaction prepared from glucosamine. Japanese J Nutr 1986;44:307-15.
15. Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavenoid content of some medicinal plants from Iran. Afr J Biotechnol 2008;7:3188-92.
16. Sathiyaseelan K, Stella D. Isolation and screening of α-glucosidase enzyme inhibitor producing marine actinobacteria isolated from pitch avaram mangrove. Int J Pharma Biol Arch 2012;3:1142-9.
17. Pratibha S, Thenmozhi M, Kannabiran K. Screening of glycolytic enzyme inhibitory activity of Streptomyces isolates from brine spring and marine sediments of India. Int J Pharma Res Rev 2013;2:5-11.
18. Selvam K, Visnupuria B, Bose SC. Screening and quantification of marine actinomycetes producing industrial enzymes amylase, cellulase and lipase from south coast of India. Int J Pharm Biol Arch 2010;2:1481-5.
19. Kelly KL. Centroid notations for the revised ISCC-NBS color name blocks. J Res Nat Bur Standards USA 1958;61:427.
20. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29:319-22.
21. Staneck JL, Roberts GD. A simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226-31.
22. Kimura MA. Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20.
23. Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
24. Zhong K, Gao XL, Xu ZJ, Gao H, Fan S, Yamaguchi I, et al. Antioxidant activity of a novel Streptomyces strain Eri12 isolated from the rhizosphere of rhizoma curcumae longae. Curr Res Bacteriol 2011;4:63-72.
25. Saha MR, Alam MA, Akter R, Jahangir R. In-vitro free radical scavenging activity of Ixora coccinea L. Bangladesh J Pharmacol 2008;3:90-6.
26. Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 2006;94:550-7.
27. Markovic ZS, Manojlovic NT. Analytical characterization of lichexanthone in lichen: HPLC, UV spectroscopic and DFT analysis of lichexanthone extracted from Laurera benguelenisis (Mull. Arg.). Zahlbr Monatshefte Chemie 2010;141:945-52.
28. Loukopoulos D. Combined therapy with deferiprone and desferrioxamine in thalassemia major. Hematol J 2005;90:1304-5.
29. Stuart AR, Gulve EA. Chemistry and biochemistry of type 2 diabetes. Chem Rev 2004;104:1255-82.
30. Franson K, Rossner S. Fat intake and food choices during weight reduction with diet, behavioural modification and a lipase inhibitor. J Int Med 2000;247:607-14.
31. Yun W. Possible anti-obesity therapeutics from nature–a review. Phytochem 2010;71:1625-41.
280 Views | 1304 Downloads
How to Cite
Bhosale, H., P. Bismile, T. Kadam, and U. Shaheen. “ANTIOXIDANT, ENZYME INHIBITORY AND ANTIFUNGAL ACTIVITIES OF ACTINOMYCETES ISOLATED FROM CURCUMA LONGA RHIZOSPHERE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 2, Feb. 2016, pp. 307-11,
Original Article(s)