• A. Hora Deputy Dean, G. S. Gill Research Institute, Guru Nanak College, Chennai, India 600042, 2 Academic advisor school of Life Sciences, Jaipur National University, Jaipur, India 302017
  • C. P. Malik National University
  • B. Kumari Vinoba Bhave University


Objective: The main aim of this study was to assess genetic diversity and phylogenetic relationships of different varieties of fenugreek (8 varieties and 6 populations) collected from northern India using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat markers).

Methods: DNA Samples were analyzed with 400 RAPD and 100 ISSR primers. For statistical analysis data were subjected to UPGMA (Un weighted pair-group method with arithmetic averages), Jaccard's similarity coefficient values were obtained using Similarity for Qualitative Data (SIMQUAL), to find out genetic relationship. This analysis generated dendrogram which was further compared using the Mantel matrix correspondence test (NTSyS-pc version 2.02i) and Principal coordinates analysis.

Results: Polymorphism frequency of 42.91% and 55.66% was found in different varieties of fenugreek using RAPD and ISSR markers respectively. Based on the combined data of RAPD+ISSR marker system the maximum similarity index was observed for accessions from Mathura and RMT-143 (0.85) whereas the minimum similarity index was observed for RMT-351 sand RMT-303 (0.26).

Conclusion: The investigation has demonstrated that cluster analysis could be profitably used in unravelling the genetic variation within the accessions and the two molecular markers, RAPD and ISSR could be used as effective tools to evaluate genetic diversity and assess genetic relationship in fenugreek.


Keywords: Genetic diversity, ISSR, Polymorphism, RAPD, Trigonella foenum-graecum L


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1. Balodi B, Rao RR. The genus Trigonella L. (Fabaceae) in the northwest himalaya. Econ Tax But 1991;1:11-6.
2. Petropoulos GA. Fenugreek-the genus trigonella. In: Petropou-los GA. Ed. 1st ed. London and New York: Taylor and Francis; 2002. p. 127.
3. Polhil RM, Raven PH. Advances in legume systematic. Royal Botanical Gardens part 2, Kew, England; 1981.
4. Darlington CD, Wylie AP. Chromosome atlas of flowering plants. In: Darlington CD. Ed. 1st ed. London: George Allen and Unwin Ltd; 1945. p. 161.
5. Snehlata HS, Payal DR. Fenugreek (Trigonella foenum-graceum L.). an overview. Int J Curr Pharm Rev Res 2011;2 Suppl 4:169:87.
6. Naidu MM, Shyamala, Naik JP, Sulochanamma G, Srinivas P. Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. Food Sci Tech 2010;44 Suppl 2:451-6.
7. Palaniswamy M, Praddep BV, Sathya R, Angayarkanni J. In vitro antiplasmodial activity of Trigonella foenum-graecum L. eCAm advance access published; 2008. p. 1-5.
8. Navayath S, Thiyagarajan D. Aqueous extract of Trigonella foenum-graecum L. prevents cypermethrin induced hepatotoxicity and nephrotoxicity. SAGE J 2010;29 Suppl 4:311-9.
9. Laroubi A, Touhami M, Farouk L, Zrara I, Aboufatima R, Ben-harref A, et al. Prophylaxis effect of Trigonella foenum-graecum L. seeds on renel stones formation in rats. Phytother Res 2007;21 Suppl 10:921-5.
10. Duke AJ. Handbook of legumes of world economic importance. New York and London: Plemus Press; 1986. p. 345.
11. Basu SK, Thomas JE, Acharya SN. Prospect for growth in global neutraceutical and functional food markets: a canadian per-spective. Aust J Basic Appl Sci 2007;1 Suppl 4:637:49.
12. Williams JGK, Kubelic AR. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 1990;18:6531-5.
13. Gept P. The use of molecular and bi°Chemical markers in crop evolution studies. Evol Biol 1993;27:51-94.
14. Karp A, Kresovich S, Bhat KV, Ayad WG, Ipgri. Molecular tools in plant genetic re-sources conservation: A guide to the tech-nologies. In: Bulletin No International Plant Genetic Resources Institute Rome; 1997. p. 2.
15. Chapco W, Ashton NW, Martel RK, Antonishyn N. A feasibility study of the use of random amplified polymorphic DNA in the population genetics and systematic of grasshoppers. Genome 1992;35:569-74.
16. Landry BS, Dextraze L, Boivin G. Random amplified polymor-phic DNA markers for DNA fingerprinting and genetic variabil-ity assessment of minute parasitic wasp species (Hymenoptera: Mymaridae and Trichogrammatidae) used in biological control programs of phtophagous insects. Genome 1993;36:580-7.
17. Demeke T, Lynch DR, Kawchuk LM, Kozub GC. Genetic diversity of potato determined by random amplified polymorphic DNA analysis. Plant Cell Rep 1996;15:662-7.
18. Li L, Xiaoying Z, Klocke E. Variation in some Lycopersicon escu-lentum and Capsicum annuum cultivars revealed by RAPD and AFLP markers. Guangxi Sci 2004;11:249-57.
19. Cheng KT, Chang HC, Su CH, Hsu FL. Identification of dried rhizomes of Copties species using random amplified polymor-phic DNA. Bot Bull Acad Sin 1997;38:241-4.
20. Carelli BP, Gerald L TS, Grazziotin FG, Echeverrigaray S. Genetic diversity among Brazilian cultivars and landraces of tomato Lycopersicon esculentum Mill. revealed by RAPD markers. Genet Resour Crop Evol 2006;53:395-400.
21. Doyle J, Doyle JL. Isolation of plant DNA from fresh tissue. F°Cus 1990;12:13-5.
22. Williams JGK, Kubelic AR. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 1990;18:6531-5.
23. Rohlf FJ. NTSYS-pc. Numerical taxonomy and multivariate analysis system. version 2.02. Exeter software: New York; 2000.
24. Dangi RS, Lagu MD, Choudhary LB, Ranjekar PK, Gupta VS. Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers. BMC Plant Biol 200;4:13.
25. Sundaram S, Purwar S. Assessment of genetic diversity among fenugreek (Trigonella foenum graceum L.) using RAPD molecular markers. J Med Plants Res 2011;5 Suppl 9:1543-8.
26. Tripathi N, Chouhan DS, Saini N, Tiwari S. Assessment of ge-netic variations among highly endangered medicinal plant Ba-copa monnieri (L.) from central India using RAPD and ISSR analysis. Biotechnology 2012;2:327-36.
27. Williams JGK, Kubelic AR. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 1990;18:6531-5.
28. Marzougui N, Boubaya A, Ines Thabti, Walid El falleh, Ferdaous Guasmi, Ali Ferchichi. Polyploidy induction of Tunisian Trigonella foenum-graecum L. populations. Afr J Biotechnol 2011;10 Suppl 43:8570-7.
29. Marzouk RI, El-Bakatoushi R. Assessment of rel°Cation of Trigonella cylindraceae L and T. polyceratia (l.) Trautv. To ge-nus Medicago as inferred by RAPD and RFLP analyses. Pak J Bot 2011;43 Suppl 22:89-94.
30. Naik A, Akhtar S, Pandey VP. Studies on genetic variability and character ass°Ciation in Fenugreek (Trigonella foenum-graecum L.). J Plant Sci Res 2013;29 Suppl 1:1-3.
31. Tomar RS, Parakhia MV, Rathod VM, Thakkar GR, Golakiya BA. A comparative analysis of RAPD and ISSR markers for studying genetic diversity in Trigonella foenum graceum genotypes. Res J Biotech 2014;9:89-95.
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How to Cite
Hora, A., C. P. Malik, and B. Kumari. “ASSESSMENT OF GENETIC DIVERSITY OF TRIGONELLA FOENUM-GRACEUM L. IN NORTHERN INDIA USING RAPD AND ISSR MARKERS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 179-83,
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