EXTRACTION, PURIFICATION AND PHYSICOCHEMICAL EVALUATION OF MUCILAGE OF CHRYSOPHYLLUM LANCEOLATUM (BLUME) DC FRUITS: AN INVESTIGATION FOR BIOADHESIVE PROPERTY

  • Amal Kumar Boruah Institute of Pharmacy, Silchar Medical College and Hospital, Silchar-14, Assam
  • Lila Kanta Nath Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam

Abstract

Objective: The objective of the present investigation is to extract, purify and characterize the physicochemical properties of the mucilage obtained from the fruits of Chrysophyllum lanceolatum (Blume) DC and also to evaluate the bioadhesiveness of Chrysophyllum lanceolatum Mucilage (CLM) to be used as the biodegradable mucoadhesive agent.

Methods: The mucilage was extracted, purified and identified by the methods as described in the official books. Purified CLM was characterized for physicochemical properties, swelling capacity, loss on drying and flow properties. The CLM was further characterized by Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC) and High-Performance Liquid Chromatography (HPLC). The mucoadhesivity of the mucilage was assessed by shear stress method and falling sphere method using goat intestine as mucosal substrate and the results were compared with Hydroxy Propyl Methyl Cellulose (HPMC) and Carbopol 934P (CP). The acute oral toxicity study was also conducted in Swiss Albino mice.

Results: The nature of the CLM is confirmed as polysaccharide from the experimental data. The physicochemical property and toxicity study also shows its acceptability as bioadhesive excipient. The bioadhesive test of CLM showed a good adhesive strength to the biological membrane.

Conclusion: The present investigation showed better bioadhesive property of the isolated mucilage and hence may provide an alternative to conventional synthetic/semi-synthetic mucoadhesive agents.

Keywords: Chrysophyllum lanceolatum, Mucilage, Extraction, Physicochemical, Bioadhesivity, Mucosal.

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Author Biography

Amal Kumar Boruah, Institute of Pharmacy, Silchar Medical College and Hospital, Silchar-14, Assam
Pharmaceutics, Lecturer

References

1. Smart JD. The basics and underlying mechanisms of mucoadhesion. Adv Drug Delivery Rev 2005;57:1556-68.
2. Garg S, Vasir JK, Tambweker K. Bioadhesive microspheres as a controlled drug delivery system. Int J Pharm 2003;255:13-22.
3. Huang Y, Leobandung W, Foss A, Peppas NA. Molecular aspects of muco-and bioadhesion: their structures and site-specific surfaces. J Controlled Release 2000;65:63-71.
4. Achar L, Peppas NA. Preparation, characterization and mucoadhesive interaction of poly (methacrylic acid) copolymers with rat mucosa. J Controlled Release 1994;32:271-95.
5. Park K, Park H. Test methods of bioadhesion, In: Lenaerts V, Gunny R. eds. Bioadhesive Drug Delivery Systems, CRC Press: Boca Raton, FL; 1990. p. 43-67.
6. Ahuja A, Khar RK, Ali J. Fundemental aspects of conventional drug therapy. Drug Dev Ind Pharm 1997;23:489-515.
7. Khosla R, Davis SS. Effects of polycarbophil on the gastric emptying pellets. J Pharm Pharmacol 1987;39:47-64.
8. Avachat AM, Dash RR, Shrotriya SN. Recent investigations of plant-based natural Gums, mucilages and resins in novel drug delivery systems. Indian J Pharm Education Res 2011;45:86–99.
9. The Wealth of India. “A dictionary of Indian Raw materials & Industrial products”, Raw Materials. Vol. II. Council of Scientific and Industrial Research, New Delhi; 1950. p. 150-1.
10. Patini B, Borah A. “Wild edible plants of Assam” First Edition, March, published by Director, Forest Communication, Forest Department, Govt. of Assam; 2007. p. 243.
11. Chandrika UG, Jansz ER, Warnasuriya ND. Identification and HPLC quantification of carotenoids of the fruit pulp of Chrysophyllum roxburghii. J Natl Sci Found Sri Lanka 2005;33:93-8.
12. Kanjilal UN, Das A, Kanjilal PC, De RN. Flora of Assam. Vol. III. Published under the authority of Assam, Prabari Press; 1939. p. 189-90.
13. Prashith TR, Raghavendra HL, Mallikarjun N, Anil HS, Vinayaka KS. Elemental analysis and biological activities of Chrysophyllum roxburghii G. Don (Sapotaceae) Leaves. Sci Technol Arts Res J 2014;3:14-20.
14. Ram PR, Mahrotra BN. Compendium of indian medical plants. Vol. 5. Central Drug Research Instt. Lucknow and National Institution of Scientific Communication, New Delhi: 1998. p. 204.
15. Ahad HA, Sreeramulu J, Bindu VH, Ramyasree P, Padmaja BS Sravanth M. Isolation and Physicochemical characterization of Ficus reticulata fruit mucilage. Int J Green Pharm 2011;5:131-4.
16. Padmakumari P, Anupama Ch, Abbulu K, Pratyusha AP. Evaluation of fruit calyces mucilage of Hibiscus Sabdariffa Linn as tablet binder. Int J Res Pharm Biomed Sci 2011;2:516-9.
17. Ojha A, Satheesh Madhav NV. Isolation and characterization of novel mucoadhesive biomaterial from Phoenix dactylifera. Int Curr Pharm J 2012;1:205-8.
18. Pharmacopoeia of India. Vol. II. Ministry of Health and Family welfare. Govt. of India. New Delhi: Controller of Publications; 1985. p. A-73, A-88.
19. Malsawmtluangi C, Thanzami K, Lalhlenmawia H, Selvan V, Palanisamy S, Kandasamy R, et al. Physicochemical characteristics and antioxidant activity of Prunus cerasoides D. Don gum exudates. Int J Biol Macromol 2014;69:192-9.
20. Singh AK, Singh A, Satheesh Madhav NV. Extraction and Physicochemical characterization of novel muco-bioadhesive biopolymer obtained from the seeds of Momordica charantia. World J Pharm Pharm Sci 2014;3:1097-115.
21. Pharmacopoeia of India. Vol. 2. Ministry of Health and Family welfare, Govt. of India, New Delhi: Controller of Publications; 1996. p. A-100.
22. British Pharmacopoeia. Vol. 2. 3rd ed. London: Her Majesty's Stationary Office for the Department of Health; 2000. p. A-207, A-210.
23. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956;28:350-6.
24. Brummer Y, Cui SW. Understanding carbohydrate analysis. In: Cui SW (Ed). Food carbohydrates: chemistry, physical properties and applications. CRC. Florida; 2005. p. 67-104.
25. Roy SK, Prabhakar B. Bioadhesive polymeric platforms for transmucosal drug delivery systems. Trop J Pharm Res 2010;09:91-104.
26. Peh KK, Wong CF. Polymeric films as vehicle for buccal drug delivery: swelling, mechanical and bioadhesive properties. J Pharm Pharm Sci 1999;2:53-61.
27. Rao M, Vani Y, Bala G, Chary R. Design and evaluation of mucoadhesive drug delivery systems. Indian Drugs 1999;35:558-65.
28. Teng CLC, Ho NFH. Mechanistic studies in the simultaneous flow and adsorption of polymer-coated latex particle on intestinal mucus I: Methods and physical model development. J Controlled Release 1987;6:133-49.
29. Ranga Rao KV, Buri P. A novel in situ method to test polymers and coated microparticles for bioadhesion. Int J Pharm 1989;52:265-70.
30. OECD 425, OECD guideline for testing of chemicals. Acute oral toxicity-Up and Down Procedure: OECD Publishing Paris; 2001.
31. Parodi B, Russo E, Gatti P, Cafaggi S, Bignardi G. Development and in-vitro evaluation of buccoadhesive tablets using a new model substrate for bioadhesion measures: the eggshell membrane. Drug Dev Ind Pharm 1999;25:289-95.
32. Rowe RC, Sheskey PJ, Owen SC. Handbook of pharmaceutical excipients. 5th ed. Pharmaceutical Press: London; 2006. p. 1, 785.
33. Nep EI, Conway BR. Physicochemical characterization of grewia polysaccharide gum: Effect of drying method. Carbohydr Polym 2011;84:446-53.
34. Marshall K. Compression and consolidation of powdered solids. 3rd ed. In: Lachman L, Lieberman HA, Kanig JL. Editors. The theory and Practice of Industrial Pharmacy, Lea and Febiger, Philadelphia: 1990. p. 66-77.
35. Vinod VTP, Saashidhar RB, Suresh KI, Rama Rao B, Vijaya sarathi UVR, Rao PT. Morphological, physicochemical and structural characterization of gum kondagogu (Cochlospermum gossypium): A tree gum from India. Food Hydrocolloids 2008;22:899-915.
36. Khonkar P. Composition and partial structure characterization of Tremella polysaccharides. Mycobiology 2009;37:286-94.
37. Cerqueira MA, Souza BWS, Simoes J, Teixeira JA, Domingues MRM, Coimbra MA, et al. Structural and thermal characterization of galactomannans from nonconventional sources. Carbohydrate Polymers 2011;83:179-85.
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Boruah, A. K., and L. K. Nath. “EXTRACTION, PURIFICATION AND PHYSICOCHEMICAL EVALUATION OF MUCILAGE OF CHRYSOPHYLLUM LANCEOLATUM (BLUME) DC FRUITS: AN INVESTIGATION FOR BIOADHESIVE PROPERTY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 2, Feb. 2016, pp. 282-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/9382.
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