EVALUATION OF IN-VITRO ANTIHELMINTIC PROPERTIES OF THE EXTRACT OF RUSSELIA EQUISETIFORMIS (SCHLECHT. AND CHAM.) SCROPHULARIACEAE


S. C. Jayaramu, M. Prathiba

Abstract


Objective: The present study was aimed to evaluate the in vitro anthelmintic activity of Russelia equisetiformis leaves in comparison with anthelmintic drug Piperazine citrate.

Methods: In vitro Anthelmintic activity of different solvent extracts (petroleum ether, chloroform, ethyl acetate and methanol) of Russelia equisetiformis were carried out on mature Pheretima posthuma.

Results: The results of the study revealed that all the solvent extracts produced a significant anthelmintic activity in a dose-dependent manner. Methanol extract offered a good anthelmintic activity at 15 mg/ml concentration, where the time of paralysis was 15 and death was observed at 27 min, followed by ethyl acetate extract. The negative control DMF did not show any activity and positive control standard piperazine citrate recorded death at 13 min and paralysis at 10 min.

Conclusion: Despite ample evidence of anthelmintic properties of R. equisetiformis through validated experimental data, further studies on Russelia equisetiformis is required to recognize the active principle responsible for the anthelmintic activity.

 


Keywords


Russelia equisetiformis, Pheretima posthuma, Anthelmintic, Solvent extracts

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References


Hossain E, Chandra G, Nandy AP, Mandal SC, Gupta JK. Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum. Parasitol Res 2012;110:809-14.

Wen LY, Yan XL, Sun FH, Fang YY, Yang MJ, Lou LJ. A randomized, double-blind, multicenter clinical trial on the efficacy of ivermectin against intestinal nematode infections in China. Acta Trop 2008;106:190-4.

Lone BA, Bandh SA, Chisti MZ, Bhat FA, Tak H, Nisa H. Anthelmintic and antimicrobial activity of methanolic and aqueous extracts of Euphorbia helioscopia L. Trop Anim Health Prod 2013;45:743-9.

Tripathi KD. Essentials of medical pharmacology. 6th ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2008. p. 808-10.

Piyush Jain Y, Rupali S. A review on anthelmintic drugs and their future scope. Int. J Pharm Pharm Sci 2011;3:17-21.

Hordegen PH, Hertzberg J, Heilmann W, Langhans VM. The anthelmintic efficacy of five plant products against gastrointestinal trichostrongylids in artificially infected lambs. Vet Parasitol 2003;117:51-60.

McGaw LJ, Jager AK, Van SJ. Antibacterial, anthelmintic and antiamoebic activity in South African medicinal plants. J Ethnopharmacol 2000;72:247-63.

Aremu AO, Ndhlala AR, Fawolem OA, Lightm ME, Finniem JF, Van Staden J. In vitro pharmacological evaluation and phenolic content of ten South African medicinal plants used as anthelmintics. S Afr J Bot 2010;76:558-66.

Pone WJ, Payne VK, Mbogning TG, Komtangi MC, Yondo J, Ngangou AM, et al. In vitro anthelmintic efficacy of Dichrocephala integrifolia (Asteraceae) extracts on the gastrointestinal nematode parasite of mice: Heligmosomoides bakeri (Nematoda, Heligmosomatidae). Asian Pac J Trop Biomed 2013;3:100-4.

Riaz M, Rasool N, Bukhari IH, Shahid M, Zahoor AF, Gilani MA, et al. Antioxidant, antimicrobial and cytotoxicity studies of Russelia equisetiformis. Afr J Microbiol Res 2012;6:5700-7.

Kolawole OT, Kolawole SO. Effects of Russelia equisetiformis methanol and aqueous extracts on hepatic function indices. Bureau Land Management 2010;2:38-41.

Harborne JB. Phytochemical methods, Chapman and Hall Publications: London; 1998. p. 7-8.

Ghosh K, Bhattacharya TK. Chemical constituents of Piper betle Linn. (Piperaceae) roots. Molecules 2005;10:798-2.

Mali RG, Mehta AA. A review on anthelmintic plants. Indian J Nat Prod Resour 2008;7:466-75.

WHO. The World Health Report: Making a Difference. Geneva; 1999. p. 122.

Roos MH. The role of drugs in the control of parasitic nematode infections: must we do without. Parasitology 1997;114(Suppl):137-4.

Okpekon T, Yolou S, Gleye C, Roblot F, Loiseau P, Bories C, et al. Antiparasitic activities of medicinal plants used in Ivory Coast. J Ethnopharmacol 2004;90:91-7.

Molgaard P, Nielsen SB, Rasmussen DE, Drummond RB, Makaza N, Andreassen J. Anthelmintic screening of zimbabwean plants traditionally used against Schistosomiasis. J Ethnopharmacol 2001;74:257-4.

Adate PS, Parmesawaran S, Yamani C. In vitro anthelmintic activity of stem extracts of Piper betle Linn. against Pheritima Posthuma. Pharmacogn J 2012;4:61-9.

Kushwaha R, Swati P, Nilambari P. Anthelmintic activity of the combination of Piper betle and Moringa oleifera inventi rapid. Planta Activa 2011;68:11.

Kane SR, Mohite SK, Shete JS. Anthelmintic activity of aqueous and methanolic extracts of Euphorbia thymifolia Linn. Int J Pharm Tech Res 2009;1:666-9.

Majeed AS, Menaka H, Basha KAN. Investigation of in vitro anthelmintic activity of Euphorbia heterophylla against Pheretima posthuma. J Pharm Toxicol 2011;1:48-52.

Tandon V, Yadav AK, Roy B, Das B. Phytochemicals as the cure of worm infections in traditional medicine systems emerging trends in zoology. Narendra Publishing House; 2011. p. 351-78.




About this article

Title

EVALUATION OF IN-VITRO ANTIHELMINTIC PROPERTIES OF THE EXTRACT OF RUSSELIA EQUISETIFORMIS (SCHLECHT. AND CHAM.) SCROPHULARIACEAE

Keywords

Russelia equisetiformis, Pheretima posthuma, Anthelmintic, Solvent extracts

Date

02-12-2015

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 8, Issue 1, 2016 Page: 459-461

Online ISSN

0975-1491

Authors & Affiliations

S. C. Jayaramu
University of Mysore
India

M. Prathiba
Department of Zoology, Yuvaraja’s College, University of Mysore, Mysore 570005, Karnataka, India


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