EXTRACTION AND SUGAR COMPOSITION OF MUCILAGE IN URGINEA INDICA/DRIMIA INDICA (ROXB) KUNTH HYACINTHACEAE

  • Banani Misra Janana Bharati Campus, Bangalore University Bangalore 560056
  • Shiva Kameshwari M. N. Department of Botany, Janana Bharati Campus, Bangalore University Bangalore 560056

Abstract

Objective: Urginea indica (U. indica) Kunth. Hyacinthaceae has high mucilage content; the aim of the study is to isolate the mucilage and to analyze the sugar present in mucilage and its Physicochemical properties.

Methods: The mucilage of U. indica extracted from the dried powder from bulbs of Urginea indica using Acetone Precipitation method. HPLC analysis has been made to see the presence of sugars in mucilage. The composition of polysaccharide analyzed based on the standard hydrolysis procedure using hydrochloric, sulfuric, and trifluoroacetic acid (TFA) at elevated temperature.

Results: The percentage yield of mucilage was found to be 4 %. The HPLC analysis revealed the presence of sugar such as Sucrose, Maltose, Fructose and Galactose in mucilage sample among which maltose was found to be higher with 21.84 µg/ml followed by Fructose (1.90 µg/ml), Galactose (2.09 µg/ml) and sucrose (0.82 µg/ml).

Conclusion: The present investigation showed that U. indica mucilage has high pharmaceutical significance with the presence of high amount of maltose, which makes it an attractive source of carbohydrate.

Keywords: Urginea indica, Mucilage, Sugar, Acetone Precipitation method, HPLC, Maltose

Downloads

Download data is not yet available.

Author Biographies

Banani Misra, Janana Bharati Campus, Bangalore University Bangalore 560056

Department of Botany

Research Scholar

Bangalore University Bangalore

Shiva Kameshwari M. N., Department of Botany, Janana Bharati Campus, Bangalore University Bangalore 560056

Department of Botany

Assistant Professor

Bangalore University Bangalore-560056

References

1. Louria DB, McAnally JF, Laser N, Lavenhar M. Onion extract in the treatment of hypertension and hyperlipidemia a preliminary communication. Curr Ther Res 1985;37:127–31.
2. Kendler BS. Garlic (Allium sativum) and onion (Allium cepa) a review of their relationship to cardiovascular disease. J Prev Med 1987;16;670–85.
3. Dorant E, Van Der Brandt PA, Goldbohm RA, Sturmans F. Consumption of onions and a reduced risk of stomach carcinoma. J Gastroenterol 1996;110:12–20.
4. Marx J, Pretorius E, Bester MJ. Effects of Urginea sanguinea, a traditional asthma remedy, on embryo neuronal development. J Ethnopharmacol 2006;104:315-21.
5. Brodnitz MH, Pascale JV. Flavour components of garlic extract.
J Agric Food Chem 1971;19:273-5.
6. Benkeblia N. Antimicrobial activity of essential oil extracts of various Onions (Allium cepa) and Garlic (Allium sativum). Food Sci Technol Res 2004;2:263-8.
7. Deepak AV, Thippeswamy G, Shivakameshwari MN, Salimath BP. Isolation and characterization of 29kDa glycoprotein from bulbs of Urginea indica. Biochem Biophys Res Commun 2006;88:297-307.
8. Ebrahimzadeh H, Niknam V, Maassoumi AA. Mucilage content and its sugar composition in Astragalus species from Iran. Pak J Bot 2000;32:131-40.
9. Banker GS, Anderson NR. Theory and practice of industrial pharmacy. Varghese Publishing House Mumbai 1987;3:296-303.
10. Narkhede Sachin B, Vidyasagar G, Jadhav Anil G, Bendale Atul R, Patel Kalpen N. Isolation and evaluation of mucilage of Artocarpus heterophyllus as a tablet binder. J Chem Pharm Res 2010;2:161-6.
11. Hadley EH. McGraw-hill encyclopedia of science and technology. McGraw-Hill Inc New York; 1997. p. 730-1.
12. Smith F, Montgomery R. The chemistry of plant gums and mucilage. Van Nostrand-Reinhold Princeton NJ; 1959. p. 98.
13. Kokate CK, Radwan SS. Mucilage in callus culture of higher plants. Phytochem 1979;18:662-3.
14. Franz G. Polysaccharides in pharmacy current application and future concepts. Planta Med 1989;55:493-7.
15. Clarke AE, Andreson RL, Stone BA. Form and function of arabinogalactans and arabinogalactan-proteins. Phytochem 1979;18:521–40.
16. Davis KR, Darvill AG, Albersheim P, Dell A. Host-pathogen interactions Oligogalacturonides released from sodium poly pectate by endo polygala-cturonic acid lyase is elicitors of phytoalexins in soybean. Plant Physiol 1986;80:568–77.
17. Zimmermann U, Zhu JJ, Meinzer FC, Goldstein G, Schneider H, Zimmermann G. High molecular weight organic compounds in the xylem sap of mangroves implications for long-distance water transport. Bot Acta 1994;107:218–9.
18. Deore SL, Khadabadi SS. Standardization and Pharmaceutical evaluation of Chlorophytum borivilianum Mucilage. Rasayan J Chem 2008;1:887-92.
19. Zakaria Boual, Abdellah Kemassi, Aminata Ould El Hadj Khelil, Philippe Michaud, Mohammed Didi Ould El Hadj. Partial characterization and hydrolysis procedure of water soluble polysaccharides extracted from onesaharian medicinal plant malvaaegyptiaca L. Int J Biosci Biochem Bioinf 2012;2:100-3.
20. Wang Q, Fang Y. Analysis of sugars in traditional Chinese drug. J Chromatogr B: Biomed Sci Appl 2004;812:309–24.
21. Sudzuki F, Munoz C, Berger H. Agro-industrial utilization of cactus pear. Food and agricultural organization of united Nation; 1993. p. 88.
22. Trachtenberg S, Mayer AM. Composition and properties of Opuntia ficus indica. Phytochem 1981;20:2665-8.
23. Josias H Hamman. Composition and application of Aloe Vera leaf gel. Molecules 2008;13:1599-616.
24. Gutterman Y, Shem-Tov S. Structure and function of the mucilaginous seed coats of plantago coronopus inhabiting the negev desert of israel. Isr J Plant Sci 1996;44:125-34.
25. Patwardhan S, Maheshwari D, Upadhyay N. Isolation, Characterization and study of disintigation properties of mucilage from Urginea indica, Liliaceae. Inventi Rapid Novel Exapients 2012;3:1-6.
26. Matsuhiro B, Luis E Lillo, Carmen Saenz, Carlos C Urzua, Oriette Zarate. Chemical characterization of the mucilage from fruits of Opuntia ficus indica. Carbohydr Polym 2006;63:263-7.
27. Sepulveda E, Saenz C, Aliaga E, Aceituno C. Extraction and characterization of Mucilage in Opuntia species. Alger J Arid Environ 2007;68:534-45.
28. Rishava malviya, Pranati Srivastava, Kulkarni GT. Applications of mucilage in drug delivery-A review. Adv Biol Res 2011;1:01-7.
29. Karawya MS, Wassel GM, Baghdadi HH, Ammar NM. Mucilage and pectins of Opuntia, Tamarindus and Cydonia. J Med Plant Res 1980;40:68-75.
30. Naod Gebresamoul, Tsige Gebre Mariam. Comperative physico chemical characterization of the mucilages of two cactus pears (Opuntia spp) obtained from Mekelle, Northern Ehhiopia. J Biomater Nanobiotechnol 2012;3:79-86.
31. Yan Lu, Thomas D Sharkey. The importance of maltose in transitory starch Break down. Plant Cell Environ 2006;29:353-66.
Statistics
399 Views | 889 Downloads
How to Cite
Misra, B., and S. K. M. N. “EXTRACTION AND SUGAR COMPOSITION OF MUCILAGE IN URGINEA INDICA/DRIMIA INDICA (ROXB) KUNTH HYACINTHACEAE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 2, Feb. 2016, pp. 335-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/9434.
Section
Short Communication(s)