• Pandita Nancy Sunandan Divatia School of Science, SVKM’s NMIMS, V. L. Mehta Road, Vile Parle West, Mumbai 400056, India
  • Vaidya Ashlesha SVKM’s NMIMS, V. L. Mehta Road


Objective: To carry out the pharmacognostic and phytochemical analysis of different extracts of seeds of Cassia absus (CA).

Methods: The CA plant material (seeds) was studied for its organoleptic, microscopic and physicochemical parameters. The seeds of CA were crushed, and their extraction was carried out in succession with 4 solvents of increasing polarities, viz hexane, ethyl acetate, alcohol, and hydro alcohol. Preliminary phytochemical screening as per standard methods was carried out on all the extracts. Thin layer chromatography of the extracts revealed their qualitative nature. Quantitative estimation of their phenol, flavonoid and sterol contents was carried out. Their antioxidant capacities were evaluated with the help of DPPH and reducing power assays. Their anti-glycation and anti-inflammatory activities were also measured.

Results: Qualitative phytochemical analysis showed the presence of alkaloids, flavonoids and steroids in the extracts. The hydroalcoholic extract showed higher phenolic content and DPPH activity among other extracts. The flavonoid, sterol and alkaloid content, and Ferric reducing power were found to be higher in ethyl acetate extract. The hexane extract displayed a higher anti-glycation activity, followed by the ethyl acetate extract.

Conclusion: A complete phytochemical screening, along with the evaluation of macroscopic and microscopic characters was carried out for 4 extracts of the seeds of Cassia absus. Quantitative estimation of flavonoid, phenol, sterol and alkaloid content was carried out. The antioxidant, anti-inflammatory, and anti-glycation capacities of the extracts were tested. Alkaloids were successfully extracted from the defatted seeds.


Keywords: Cassia absus, Seeds, Anti-oxidant, Anti-glycation, Anti-inflammatory, Alkaloids


Download data is not yet available.


1. Cassia Linn. Reviews on Indian medicinal plants, Indian Council of Medical Research. Vol. 5. New Delhi; 1994. p. 617.
2. Kirtikar KR, Basu BD. Indian medicinal plants, Sudhindranath Basu. Vol. 2. Publishers; 1918. p. 873.
3. Siddiqui S, Ahmed Z. Alkaloids from the seeds of Cassia absus. Proc Math Sci 1935;2:421-25.
4. Aftab K, Atta-Ur-Rahman, Ahmed SI, Usmanghani K. Traditional medicine Cassia absus L. (chakshu)-pharmacological evaluation. Phytomedicine 1996;2:213-9.
5. Abdul L, Abdul R, Sukul RR, Nazish S. Anti-inflammatory and antihistaminic study of a unani eye drop formulation. Ophthalmol Eye Dis 2010;2:17-22.
6. Yadav RN, Vishwakarma UK. New biologically active allelochemical from seeds of Cassia absus Linn. Indian J Chem B 2013;52:953-57.
7. Kapoor VP, Mukherjee S. Galactomannan from Cassia absus seed. I. Nature of sugars present, methylation, and periodate oxidation studies. Can J Chem 1969;47:2883-7.
8. Kapadia Z, Fernandes L. Isolation of raffinose from Cassia absus Linn. J Chem Soc 1986;8:429-30.
9. Patel GK, Gupta AK, Gupta A, Mishra M, Singh PK, Saxena AK, et al. Purification and physicochemical characterization of a Trypsin Inhibitor from Cassia absus Linn. Protein Peptide Lett 2014;21:108-14.
10. Ledwani L, Oberoi S. A comparative study of fatty acids and sterols profiles in seed oil of five Cassia species. Arch Appl Sci Res 2010;2:295-301.
11. Hosamani KM. A Rich source of novel 9-ketooctadec-cis-15-enoic acid from Cassia absus seed oil and its possible industrial utilization. Ind Eng Chem Res 1994;33:1058-61.
12. Quality control methods for medicinal plant materials. World Health Organization, Geneva; 1998.
13. Harbourne AJ. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Springer; 1998.
14. Saeed N, Khan KR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of the whole plant extracts Torilis leptophylla L. BMC Complementary Altern Med 2012;12:221.
15. Malla MY, Sharma M, Saxena RC, Mir MI, Mir AH, Bhat SH. Phytochemical screening and spectroscopic determination of total phenolic and flavonoid contents of Eclipta Alba Linn. J Nat Prod Plant Resour 2013;3:86-91.
16. Attarde D, Pawar J, Chaudhari B, Pal S. Estimation of sterols content in edible oil and ghee samples. Int J Pharm Sci Rev Res 2010;5:135-37.
17. Sreevidya N, Mehrotra S. Spectrophotometric method for estimation of alkaloids precipitable with Dragendorff’s reagent in plant materials. J AOAC Int 2003;86:1124-7.
18. Wu JW, Hsieh CL, Wang HY, Chen HY. Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein. Food Chem 2009;113:78–84.
19. Arif Ullah HM, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, et al. Evaluation of antinociceptive, in-vivo and in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complementary Altern Med 2014;14:346.
20. Sarker SD, Latif Z, Gray A. Natural product isolation. 2nd ed. Humana Press; 2006. p. 333.
21. Hamedi A, Khoshnoud MJ, Tanideh N, Abbasi F, Fereidoonnezhad M, Mehrabani D. Reproductive toxicity of Cassia absus seeds in female rats: possible progesteronic properties of chaksine and b-sitosterol. Pharm Chem J 2015;49:268-74.
22. Pandya H, Kachwala Y, Sawant L, Pandita N. Pharmacognostical screening of seeds of Cassia absus. Pharmacogn J 2010;2:420-26.
23. Jayaraman. Comparative analysis of antioxidant activity and phytochemical potential of Cassia absus Linn., Cassia auriculata Linn. and Cassia fistula Linn. Indian J Drugs Diseases 2014;3:298-304.
24. Odjakova M, Popova E, Al Sharif M, Mironova R. Plant-derived agents with anti-glycation activity. In: Petrescu S. editor. Glycosylation. Intech; 2012. p. 223-56.
734 Views | 3536 Downloads
How to Cite
Nancy, P., and V. Ashlesha. “PHARMACOGNOSTIC AND PHYTOCHEMICAL STUDIES OF CASSIA ABSUS SEED EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 325-32,
Original Article(s)