PHARMACOGNOSTIC AND PHYTOCHEMICAL STUDIES OF CASSIA ABSUS SEED EXTRACTS
Objective: To carry out the pharmacognostic and phytochemical analysis of different extracts of seeds of Cassia absus (CA).
Methods: The CA plant material (seeds) was studied for its organoleptic, microscopic and physicochemical parameters. The seeds of CA were crushed, and their extraction was carried out in succession with 4 solvents of increasing polarities, viz hexane, ethyl acetate, alcohol, and hydro alcohol. Preliminary phytochemical screening as per standard methods was carried out on all the extracts. Thin layer chromatography of the extracts revealed their qualitative nature. Quantitative estimation of their phenol, flavonoid and sterol contents was carried out. Their antioxidant capacities were evaluated with the help of DPPH and reducing power assays. Their anti-glycation and anti-inflammatory activities were also measured.
Results: Qualitative phytochemical analysis showed the presence of alkaloids, flavonoids and steroids in the extracts. The hydroalcoholic extract showed higher phenolic content and DPPH activity among other extracts. The flavonoid, sterol and alkaloid content, and Ferric reducing power were found to be higher in ethyl acetate extract. The hexane extract displayed a higher anti-glycation activity, followed by the ethyl acetate extract.
Conclusion: A complete phytochemical screening, along with the evaluation of macroscopic and microscopic characters was carried out for 4 extracts of the seeds of Cassia absus. Quantitative estimation of flavonoid, phenol, sterol and alkaloid content was carried out. The antioxidant, anti-inflammatory, and anti-glycation capacities of the extracts were tested. Alkaloids were successfully extracted from the defatted seeds.
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