ASSESSMENT OF FREE RADICAL SCAVENGING ACTIVITY OF TEN MADHURASKANDHA DRUGS THROUGH UV SPECTROSCOPIC AND CHROMATOGRAPHIC TECHNIQUE
Objective: Acharya Charaka had classified Asthapana bastidravya (corrective enema) based on Rasa (Taste), called as Rasaskandha (a group of drugs having similar taste). He ascertained some criteria to include drugs in the group such as drug having either similar Rasa (taste) or Vipaka (biotransformation) or Prabhava (principle responsible for a specific action). The study was planned to endorse the grouping of Madhuraskandha based upon Rasayana karma (rejuvenation) vis a vis antioxidant activity as well as to evaluate the natural source of antioxidants.
Methods: The study was planned to assess the free radical scavenging activities often drugs by adopting DPPH [2, 2-di-(4-tert-octylphenol)-1-picrylhydrazyl], H2O2 (Hydrogen peroxide) and superoxide scavenging activity and FRAP (Ferric reducing antioxidant power) assay with UV spectroscopy. Further, methanolic extract often drugs was subjected to thin layer chromatography followed by DPPH technique.
Results: The aqueous extracts of Abutilon indicum, Solanum xanthocarpum, Tribulus terrestris, Boerhavia diffusa, Group A and Group C had shown potent antioxidant activity in concentration-dependent manner by illustrated methods. DPPH-TLC (thin layer chromatography) assay showed that maximum active compounds were found in methanolic extracts of Asparagus racemosus and Tinospora cordifolia.
Conclusion: The result revealed that the drugs included in the Madhuraskandha have similar attributes of Rasayana Karma or possess antioxidant activity. The combination of ten drugs can be a good source of natural antioxidants.
Keywords: Madhuraskandha drugs, Free radical scavenging activity, Spectroscopy, Chromatography
2. Acharya YT. Editor. Carakasamhita of Agnivesh, Sutrasthana 26/42,61. Ayurvedadipika commentary by Chakrapani. 1st edition. reprint, Chaukhambha Surbharati Prakashana, Varanasi; 2009.
3. Branen AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 1975;52:59-63.
4. Moure A, Cruz JM, Franco D, Manuel Dominguez J, Sineiro J, Dominguez H, et al. Natural antioxidants from residual sources. Food Chem 2001;72:145-71.
5. B Sushama, K Nishteswar. Analysis of madhuraskandha rasayana drugs through pharmacognostical, physicochemical and phytochemical parameters. Int J Ayu Pharm Chem 2015;3:46-59.
6. Anonymous. The ayurvedic pharmacopoeia of india. Part-I. Vol. 1-4. Govt. of India, Ministry of Health and FW, Dept. of ISM and H, New Delhi; 1999.
7. B Spangenberg, CF Poole, Ch Weins. The quantitative thin layer chromatography-A practical survey, Springer Heidelberg Dordrecht London, New York; 2011.
8. Ruch RJ, Cheng SJ, Klaunig JE. Preventive of cytotoxicity and inhibition of intercellular communication by antioxidant catechin isolated from Chinese green tea. Carcinogen 1989;10:1003-8.
9. Navarro CM, Montilla MP, Martin A, Jimenez J, Utrilla MP. Free radical scavenging and anti-hepatotoxic activity of Rosamarinus tomentosus. Planta Med 1993;59:312-4.
10. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44:276-87.
11. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power; the FRAP assay. Anal Biochem 1996;239:70-6.
12. Frie B, Stocker R, Ames BN. Antioxidant defenses and lipid peroxidation in human blood plasma. Proc Natl Acad Sci 1988;37:569â€“71.
13. Niki E. Antioxidant defenses in eukaryotic cells. In: Poli G, Albano E, Dianzani MU. editors. Free radicals: From basic science to medicine. Basel, Switzerland: Birkhauser Verlag; 1993. p. 365â€“73
14. Halliwell B. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. Am J Med 1991;91:14-22.
15. Kincaid-Colton, Carol Wolfgang Streit. The brain's immune system. Sci Am 1995;273:54-61.
16. Mark SM. Alger. Polymer science dictionary. Springer; 1997.
17. Kong Fanli, Zhang Mingwei, Liao Sentai, Yu Shujuan, Chi Jianwei, Wei Zhencheng. Antioxidant activity of polysaccharide-enriched fractions extracted from pulp tissue of litchi Chinensis sonn. Molecules 2010;15:2152-65.
18. K Shashank, Pandey Abhay K. Chemistry and biological activities of flavonoids: an overview. Sci World J 2013;29:1-16.
19. http://www.phytochemicals.info/ [Last accessed on 09 Oct 2015].