• Anania Arjuna Faculty of Applied Medical Sciences, Lovely Professional University, Jalandhar, India


Objective: The marine thaustochytrid Schizochytrium sp known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications. In the present study, distillery waste was studied as an alternative carbon source for DHA production.

Methods: Alcohol beverage distillery wastewater contains significant amounts of proteins and formal-type (free) amino acids, which were used as a raw material for growth of the organism and production of DHA, further effect of carbon, nitrogen, temperature, sea salt initial pH and oxygen transfer rate was studied for DHA production.

Results: In the present study, alcoholic beverage distillery wastewater was used as a medium component, which is one of the major pollutants and of concern to the environmental scientists worldwide. The DHA accumulation in the total lipid was found to be 30 % by using 1 % glucose with the productivity of 55.8 g/l of lipid. The sea salt was optimized to be used at 35 g/l which resulted in best lipid production. Further optimization studies were carried out to improve the biomass yield as well as DHA yield.

Conclusion: In the present research work it was concluded that alterations in various physiochemical conditions like carbon sources, nitrogen sources, temperature, salt concentration, pH showed a major effect on the increase in DHA production This study highlights optimum conditions for increasing DHA production by Scizochytrium sp. .The research work gives insight into the rapidly gaining high production of polyunsaturated fatty acids using a cheaper raw material.

Keywords: Distillery waste, Amino acids, Alcoholic beverages, Docosa hexanoic acid


Download data is not yet available.


Morimura S, Kida K, Sonoda Y. Production of protease using waste water from the manufacture of Shochu. J Ferment Bioeng 1994;77:183-7.

Yokochi D, Honda T, Higashihara T, Nakahara T. Optimization of docosahexaenoic acid production by Schozochytrium limacinum SR21. Appl Microbiol Biotechnol 1998;49:72–6.

Yoshimoto M, Kurata-Azuma R, Fujii M, Hou DX, Ikeda K, Yoshidome T, et al. Phenolic composition and radical scavenging activity of sweet potato-derived shochu distillery by-products with koji. Biosci Biotechnol Biochem 2004;68:2477-83.

Morimura S, Juan YX, Shigematsu T, Kida K. Production of vinegar with high physiological activities from rice-schochu distillery wastewater. Seibutsu Kogaku Kaishi 2002;80:417-23.

Yokoyama S, Hiramatsu J, Hayakawa K. Production of γ-aminobutyric acid from alcoholic distillery lees by Lactobacillus brevis IF-I2005. J Biosci Bioeng 2002;93:95-7.

Mahfudz LD, Nakashima K, Ohtsuka A, Hayashi K. Growth factors for a primary chick muscle cell culture from Shochu distillery by-products. Biosci Biotechnol Biochem 1997;61:1844-77.

Furuta Y, Takashita H, Omori T, Sonomoto K, Ishizaki A, Shimoda M, et al. Growth stimulating affect of Shochu wastewater on lactic acid bacteria and bifidobacteria. Ann N Y Acad Sci 1998;864:276-9.

Cavalier-Smith T, Allsopp MTEP, Chao EE. Thraustochytrids are chromists, not fungi: 18S rRNA signatures of Heterokonta. Phil Trans Royal Soc London B 1994;346:387-97.

Moss ST. Biology and phylogeny of the Labyrinthulates and thraustochytriales. In: Moss ST. ed. The biology of marine fungi. Cambridge University Press: New York, USA; 1986. p. 105-29.

Yongmanitchai W, Ward 0P. Omega-3 fatty acids: alternative sources of production. Process Biochem 1989;24:117-25.

Nakahara T, Yakochi T, Higashihara S, Tanaki T, Yaguchi T, Honda D. Production of docosahexaenoic and docosapentaenoic acids by Schizochytrium sp. isolated from Yap Island. J Am Oil Chem Soc 1996;73:1421–6.

Huang J, Aki T, Yokochi T, Nakahara T, Honda D, Kawamoto S, et al. Grouping newly isolated docosahexaenoic acid-producing thraustochytrids based on their polyunsaturated fatty acid profiles and comparative analysis of 18S rRNA genes. Mar Biotechnol 2003;5:450-7.

Giusto NM, Pasquare SJ, Salvador PI, Roque MG. Lipid metabolism in vertebrate retinal rod outer segments. Prog Lipid Res 2000;39:315–91.

Nettleton JA. Are n-3 fatty acids essential nutrients for fetal and infant development? J Am Dietetic Assoc 1993;93:58-64.

Crawford P. The requirements of long-chain n-6 and n-3 fatty acids for the brain. In: Lands WEM. ed. Short course in polyunsaturated fatty acids and eicosanoids. (Proceedings of the AOCS Conference) Proc Am Oil Chem Soc; 1987. p. 270–95.

Das UN, Fams MD, Long-chain polyunsaturated fatty acids in the growth and development of the brain and memory. Nutrition 2003;19:62–5.

Kang JX, Leaf A. The cardiac antiarrhythmic effects of polyunsaturated fatty acid. Lipids 1996;31:41–4.

Nordøy A, Marchioli R, Arnesen H, Videbæk J. n-3 Polyunsaturated fatty acids and cardiovascular diseases. Lipids 2001;36:127-9.

Horrocks LA, Young KY. Docosahexaenoic acid-enriched foods: production and effects on blood lipids. Lipids 1999;34:313.

Cohen Z. (In Handbook of Micraalgal Mass Culture" (A. Richmond, ed.), CRC press: BOCA Raton FL; 1986. p. 421-54.

CPCB (Central Pollution Control Board). Annual report; 2002-03.

Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911-7.

Trinder. Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Ann Clin Biochem 1969;6:24-5.

TERI (The Energy and Resources Institute) Background paper on water efficiency status in Indian distilleries and agro-based pulp and paper mills; 2003.

Singh A, Wilson S, Ward OP. Docosahexaenoic acid (DHA) production by Thraustochytrium sp. ATCC 20892. World J Microbiol Biotechnol 1996;12:76-81.

Li ZY, Ward OP. Production of docosahexaenoic acid by Thraustochytrium roseum. J Ind Microbiol 1994;13:238-41.



How to Cite

Arjuna, A. “UTILIZATION OF ALCOHOLIC BEVERAGE DISTILLERAY WASTE WATER FOR PRODUCTION OF DOCOSAHEXAENOIC ACID”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 4, Apr. 2016, pp. 333-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/9909.



Original Article(s)