UTILIZATION OF ALCOHOLIC BEVERAGE DISTILLERAY WASTE WATER FOR PRODUCTION OF DOCOSAHEXAENOIC ACID
Objective: The marine thaustochytrid Schizochytrium sp known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications. In the present study, distillery waste was studied as an alternative carbon source for DHA production.
Methods: Alcohol beverage distillery wastewater contains significant amounts of proteins and formal-type (free) amino acids, which were used as a raw material for growth of the organism and production of DHA, further effect of carbon, nitrogen, temperature, sea salt initial pH and oxygen transfer rate was studied for DHA production.
Results: In the present study, alcoholic beverage distillery wastewater was used as a medium component, which is one of the major pollutants and of concern to the environmental scientists worldwide. The DHA accumulation in the total lipid was found to be 30 % by using 1 % glucose with the productivity of 55.8 g/l of lipid. The sea salt was optimized to be used at 35 g/l which resulted in best lipid production. Further optimization studies were carried out to improve the biomass yield as well as DHA yield.
Conclusion: In the present research work it was concluded that alterations in various physiochemical conditions like carbon sources, nitrogen sources, temperature, salt concentration, pH showed a major effect on the increase in DHA production This study highlights optimum conditions for increasing DHA production by Scizochytrium sp. .The research work gives insight into the rapidly gaining high production of polyunsaturated fatty acids using a cheaper raw material.
Keywords: Distillery waste, Amino acids, Alcoholic beverages, Docosa hexanoic acid
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