FORMULATION OF IBUPROFEN LOADED ETHYL CELLULOSE NANOPARTICLES BY NANOPRECIPITATION TECHNIQUE
Objective : The present study was undertaken to prepare and characterize drug loaded Ethyl cellulose nanoparticles using nanoprecipitation technique. The model drug chosen was Ibuprofen.
Method : These nanoparticles were prepared using nanoprecpitation technique in which the organic phase containing varied proportions of drug and the polymer was added drop wise to the aqueous phase having the stabilizer with continuous stirring. This resulted in the formation of precipitate in the aqueous phase. The stirring was continued for about 2 hours further. Then, the precipitate was collected by subjecting the sample to vacuum filtration and was air-dried.
Results : Â The best formulation among all the three was determined by comparing the particle size, stability and invitro drug release of all the formulation. F3 was considered as the best formulation with average particle size as 251.1 nm, zeta potential as -25.2 mV, and 86.02 % drug release which was sustained till 8 hours.
Conclusion: Â F3 formulation i.e; in which concentration of drug and polymer bears a ratio of 1:2 was concluded as the best because of their smaller particle size and greater stability.
Keywords : Acetone, Ethyl Cellulose(EC), Ethanol, Ibuprofen, Nanoparticles, Nanoprecipitation.
2. Stephanie Homig, C.Remzi Becer and Ulrich S.Schbert. Synthetic polymeric nanoparticles by nanoprecipitation. Journal of Materials Chemistry.
3. Stephanie Schubert, Joesph T.Delaney and Ulrich S.Schubert. Nanoprecipitation and Nanoformulation of polymers: from history to powerful possibilities beyond poly(lactic acid).Soft Matter 2010.
4. Patrick Nardstrom: Formulation of polymeric nanoparticles encapsulating and releasing new hydrophobic cancer drug. Master of Science Thesis in Materials and Nanoptechnology. Sweden; 2011.
5. Avanco G B, Bruschi M.L. Preparation and Characterisation of ethylcellulose microparticles containing propolis. Journal of Basic and Applied Pharmaceutical Science 2008; 29(2):129-135.
6. Pranita Kashyap, Hemant Dhongade, Kamlesh Singh, Afreen Ansari, Pragati Bhagel. Preparation and Evaluation of Acetaminophen Microparicles. International Journal of Medical and Pharmaceutical Sciences 2011; 1(5).
7. Nepolean.R et al. Preparation and Characterization of Nisoldipine Nanoparticles by Nanoprecipitation Method. J.Pharm. Sci. & Res. 2012; 4(11):1989-1994.
8. Jose Mario Barichello, Mariko Morishita, Kozo Takayama and Tsuneji Nagai. Encapsulation of Hydrophilic and Lipophilic Drugs in PLGA Nanoparticles by the Nanoprecipitation Method. Drug Development and Industrial Pharmacy 1999; 25(4) :471-476.
9. Gupta Dilip Kumar, Razdan B.K. Bajpai Meenakshi. Formulation and Evaluation of nanoparticles containing Artemisinin HCL, International Journal of Research and Development in Pharmacy and Life Sciences. March 2014; 3(2): 925-934.
10. A.Krishna Sailaja, P.Amareshwar. Preparation of alginate nanoparticles by desovaltion technique using acetone as desolvating agent.Asian Journal of Pharmaceutical and Clinical Research. 2012; 5(2).
11. A.Krishna Sailaja, P.Amareshwar, P.Chakravarty. Different Techniques for the preparation of Nanoparticles using natural polymers and their application, International Journal of Pharmacy and Pharmaceutical Sciences.2011; 3(2).
12. The Merck Index. An Encyclopedia of Chemicals, drug and biological, Merck Laboratories, 13th Edition. 2001. Pp139.
13. Merodio M,. Arnedo A, Renedo M.J. Irache J.M, Ganciclovirâ€loaded albumin nanoparticles:characterization and in vitro release properties, European J of Pharmceutical Sciences, (2001); Vol.12(3), 251.
14. Bahareh Azimi, Parviz Nourpanah, Mohammad Rabiee and Shahram Arbab. Producing Gelatin Nanoparticless as Delivery System for Bovine Serum Albumin, Iranina Biomedical Journal. 2014; 18(1): 34-40.
15. Mohanraj VJ, Chen Y Nanoparticles â€“ A Review. Trop. J.Pharm. Res. 2006; 5(1): 561-573.
16. Radhika Ramachandran and Piranamayagam Shanmughavel. Preparation and characterization of biopolymeric nanoparticles for Drug-delivery, Indian Journal of Biochemistry and Biophysics. 2010; 47: 56-59.
17. Vyas S P, Khar R K, Targeted & Controlled Drug Delivery, Novel Carrier Systems, CBS publicarion, 2002, 331-387.
18. Wim H De Jong, Paul Ja Borm. Drug delivery and nanoparticles: Applications and Hazards, Int J Nanomedicine. June 2008; 3(2): 133-149.
19. G.M Barratt, Therapeutic Applications of colloidal drug carriers, Pharm. Sci. Technol. Today 3, 163-171.
20. Jiang B, Hu L, Gao C, Shen J. Ibuprofen loaded nanoparticles prepared by a co-precipitation method and their release properties, Int J Pharm. Nov 2005; 304(1-2): 220-30.
21. Rahimnejad M., M. Jahanshahi*, G. D. Najafpour. Production of biological nanoparticles from bovine serum albumin for drug delivery. African Journal of Biotechnology.2006; Vol. 5 (20): pp. 1918-1923.
22. Dongmei Zhao,Xiuhua Zhao,Yuangang Zu,Jialei Li Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles. International Journal of Nanomedicine 2010;5 : 669â€“677.
23. Truong-Le VL, Walsh SM, Guggino WB, August JT, Leong KW. Gene transfer by DNA-Gelatin nanospheres, Arch. Biochem. Biophys. 1999; 361(1): 47-56.
24. Galindo-Rodriguez S.A, Allemann EH, Fessi, Doelker E. Polymeric nanoparticles for oral delivery of drugs and vaccines: a critical evaluation of in vivo studies, Crit. Rev. Ther. Drug Carr.Syst. 22 (2005) 419â€“464.
25. Galindo-Rodriguez S.A, Allemann EH, Fessi, Doelker E. Polymeric nanoparticles for oral delivery of drugs and vaccines: a critical evaluation of in vivo studies, Crit. Rev. Ther. Drug Carr.Syst. 22 (2005) 419â€“464.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.