A REVIEW ON ANTIOXIDANT METHODS


Dontha Sunitha

Abstract


ABSTRACT
To provide an outlook of the various available methods of antioxidant activity. Various available in vitro and in vivo methods are listed and the
procedure to perform the method, its mechanism is also explained in brief. 1,1-diphenyl-2-picrylhydrazyl method was found to be used mostly for the
in vitro antioxidant activity evaluation purpose while lipid peroxidation was found as mostly used in vivo antioxidant assay. An ethanol was with the
highest frequency as a solvent for extraction purpose. Summarized information on the various methods available provides with reliable information
to confirm the benefits of antioxidant effects.
Keywords: Antioxidant activity, Reactive oxygen species, Free radical, 1,1-diphenyl-2-picrylhydrazyl, Flavonoid.


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References


REFERENCES

Tiwari AK. Antioxidants: New generation therapeutic base for treatment

of polygenic disorders. Curr Sci 2004;86(8):1092-102.

Velavan S. Free radicals in health and diseases. Pharmacol Online

;1(1):1062-77.

Halliwell B. Antioxidants and human disease: A general introduction.

Nutr Rev 1997;55:S44-9.

Gomberg M. An incidence of trivalent carbon trimethylphenyl. J Am

Chem Soc 1900;22:757-71.

Inoue M, Sato EF, Nishikawa M, Park AM, Kira Y, Imada I, et al.

Mitochondrial generation of reactive oxygen species and its role in

aerobic life. Curr Med Chem 2003;10(23):2495-505.

Cadenas E, Davies KJ. Mitochondrial free radical generation, oxidative

stress, and aging. Free Radic Biol Med 2000;29(3-4):222-30.

Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J. Role of oxygen

radicals in DNA damage and cancer incidence. Mol Cell Biochem

;266(1-2):37-56.

Lopaczynski W, Zeisel SH. Antioxidants, programmed cell death, and

cancer. Nutr Res 20011;21:295-307.

Glade MJ. The role of reactive oxygen species in health and disease

northeast regional environmental public health center university of

Massachusetts. Amerst Nutrition 2003;19(4):401-3.

Poli G, Leonarduzzi G, Biasi F, Chiarpotto E. Oxidative stress and cell

signalling. Curr Med Chem 2004;11(9):1163-82.

Duracková Z. Some current insights into oxidative stress. Physiol Res

;59(4):459-69.

Halliwell B. Uric acid: An example of antioxidant evaluation. In:

Cadenas E, Packer L, editors. Handbook of Antioxidants. New York:

Marcel Dekker; 1996. p. 243-56.

Rahman K. Garlic and a ging: New insights into an old remedy. Ageing

Res Rev 2003;2(1):39-56.

Halliwell B, Murcia MA, Chirico S, Aruoma OI. Free radicals and

antioxidants in food and in vivo: What they do and how they work. Crit

Rev Food Sci Nutr 1995;35(1-2):7-20.

Simon HU, Haj-Yehia A, Levi-Schaffer F. Role of reactive oxygen

species (ROS) in apoptosis induction. Apoptosis 2000;5(5):415-8.

Pierart ZC, Rozowsky NJ. Papel de la nutricion en la prevencion del

cancer gastrointestinal. Rev Chil Nutr 2006;33(1):8-13.

Pamplona-Roger GD. Encyclopedia of Foods and Their Healing Power:

A Guide to Food Science and Diet Therapy. Madrid, Spain: Editorial

Safeliz; 2006. p. 447.

Espín JC, García-Conesa MT, Tomás-Barberán FA. Nutraceuticals:

Facts and fiction. Phytochemistry 2007;68(22-24):2986-3008.

Shetti A, Keluskar V, Aggarwal A. Antioxidants enhancing oral and

general health. J Indian Acad Oral Med Radiol 2009;21(1):1-6.

Reynoso-Camacho R, Ramos-Gomez M, Loarca-Pina G. Bioactive

components in common beans (Phaseolus vulgaris L.) Adv Agric Food

Biotechnol 2006;217-36.

Duarte-Martino HC, Bigonha SM, De Morais CL, De Oliveira BR,

Brunoro CN, Ramirez CL, et al. Nutritional and bioactive compounds of

bean: Benefits to human health. In: Tunick MH, González DE, Mejía E.

editors. Hispanic Foods: Chemistry and Bioactive Compounds (ACS

Symposium). USA: American Chemical Society; 2012. p. 233-58.

Salinas-Moreno Y, Perez-Alonso JJ, Vazquez-Carrillo G, AragonCuevas

F,

Velazquez-CarDelas

GA.

Antocianinas

y

actividad

antioxidante

en

maíces

(Zea

mays

L.) Agrociencia

;46(7):693-706.

Achakittirungrod S, Okonogi S, Poonpohn SC. Study on antioxidant

activity of certain plants in Thailand: Mechanism of antioxidant action

of guava leaf extract. Food Chem 2007;103(2):381-8.

Chaturvedi S, Sharma PK, Gark VK, Bansal M. Role of nutraceuticals

in health promotion. Int J PharmTech Res 2011;3(1):442-8.

Kaisoon O, Siriamornpun S, Weerapreeyakul N, Meeso N. Phenolic

compounds and antioxidant activities of edible flowers from Thailand.

J Funct Foods 2011;3(2):88-99.

Chew YL, Chan EW, Tan PL, Lim YY, Stanslas J, Goh JK. Assessment

of phytochemical content, polyphenolic composition, antioxidant and

antibacterial activities of leguminosae medicinal plants in Peninsular

Malaysia. BMC Complement Altern Med 2011;11:12.

Bangalore DV, McGlynn W, Scott DD. Effect of beta-cyclodextrin in

improving the correlation between lycopene concentration and ORAC

values. J Agric Food Chem 2005;53(6):1878-83.

Calliste CA, Trouillas P, Allais DP, Duroux JL. Castanea sativa mill.

leaves as new sources of natural antioxidant: An electronic spin

resonance study. J Agric Food Chem 2005;53(2):282-8.

Cerda B, Espin JC. Metabolism of antioxidants and chemopreventive

ellagitanins from strawberries, raspberries, walnuts, and oak-aged wine

in humans had been reported and identification of biomarkers and

individual variability. J Med Food Chem 2005;68(8):1293.

Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study on

antioxidant activity of certain plants in Thailand: Mechanism of

antioxidant action of guava leaf extract. Food Chem 2007;103(2):381-8.

Chaturvedi S, Sharma PK, Garg VK, Bansal M. Role of nutraceuticals

in health promotion. Int J PharmTech Res 2011;3(1):442-8.

Chew YL, Chan EW, Tan PL, Lim YY, Stanslas J, Goh JK. Assessment

of phytochemical content, polyphenolic composition, antioxidant and

antibacterial activities of Leguminosae medicinal plants in Peninsular

Malaysia. BMC Complement Altern Med 2011;11(1):1-10..

Tuberoso CI, Boban M, Bifulco E, Budimir D, Pirisi FM. Antioxidant

capacity and vasodilatory properties of mediterranean food: The case of

Cannonau wine, myrtle berries liqueur and strawberry-tree honey. Food

Chem 2013;140(4):686-91.

Kontou N, Psaltopoulou T, Panagiotakos D, Dimopoulos MA, Linos A.

The mediterranean diet in cancer prevention: A review. J Med Food

;14(10):1065-78.

Fernandez-Mar MI, Mateos R, Garcia-Parrilla MC, Puertas B, CantosVillar

E. Bioactive compounds

in wine: Resveratrol, hydroxytyrosol

and

melatonin: A

review.

Food Chem 2012;130:797-813.

Balasundram N, Sundaram K, Samman S. Phenolic compounds in

plants and agri industrial by-products: Antioxidant activity, occurrence,

and potential uses. Food Chem 2006;99:191-203.

Moure A, Cruz JM, Franco D, Dominguez JM, Sineiro J, Dominguez H,

et al. Natural antioxidants from residual sources. Food Chem

;72(2):145-71.

Pérez-Bonilla M, Salido S, van Beek TA, Linares-Palomino PJ,

Altarejos J, Nogueras M, et al. Isolation and identification of radical

scavengers in olive tree (Olea europaea) wood. J Chromatogr A

;1112(1-2):311-8.

Brian DC, Adrian LK, Ryszard A, Ronald BP. Phenol-based antioxidants

and the in vitro methods used for their assessment. Compr Rev Food Sci

Food Saf 2012;11:148-73.

Winkel-Shirley B. Flavonoid biosynthesis. A colorful model for

genetics, biochemistry, cell biology, and biotechnology. Plant Physiol

;126(2):485-93.

Chouhan HS, Singh SK. Antibacterial activity of seed and flower parts

of Crotalaria juncea Linn. Am Eurasian J Sci Res 2010;5(3):212-5.

Zhishen J, Mengcheng T, Janming W. The determination of flavonoids

contents in mulberry and their scavenging effects on superoxide

radicals. Food Chem 1999;64:555-9.

Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W.

Antioxidant activity, total phenolic, and total flavaonoid of extracts

and fractions of red fruit (Pandanus conoideus Lam). Int Food Res J

;17:97-106.

Deepshikha G. Methods for determination of antioxidant capacity:

A review. Int J Pharm Sci Res 2015;6(2):546-66.

Burda S, Oleszek W. Antioxidant and antiradical activities of flavonoids.

J Agric Food Chem 2001;49(6):2774-9.

Inoue K, Murayarna S, Seshimo F, Takeba K, Yoshimura Y,

Nakazawa H. Identification of phenolic compound in manuka honey

as specific superoxide anion radical scavenger using Electron Spin

Resonance (ESR) and liquid chromatography with coulometric array

detection. J Sci Food Agric 2005;85:872-8.

Fahey JW, Stephenson KK. Pinostrobin from honey and Thai ginger

(Boesenbergia pandurata): A potent flavonoid inducer of mammalian

phase 2 chemoprotective and antioxidant enzymes. J Agric Food Chem

;50(25):7472-6.

Bondet V, Brand-Williams W, Berset C. Kinetics and mechanisms of

antioxidant activity using the DPPH free radical method. Food Sci

Technol 1997;30:609-15.

Parejo I, Codina C, Petrakis C, Kefalas P. Evaluation of scavenging

activity assessed by Co(II)/EDTA-induced luminol chemiluminescence

and DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical assay.

J Pharmacol Toxicol Methods 2000;44(3):507-12.

Ferreira IC, Aires E, Barreira JC, Estevinho LM. Antioxidant activity of

Portuguese honey samples: Different contributions of the entire honey

and phenolic extract. Food Chem 2009;114:1438-43.

Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 14-32

Dontha

Piljac-Zegarac J, Stipcevic T, Belscak A. Antioxidant properties and

phenolic content of different floral origin honeys. J Api Prod Api Med

Sci 2009;1:43-50.

Lee BC, Lee SY, Lee HJ, Sim GS, Kim JH, Kim JH, et al. Antioxidative

and

photo-protective

effects

of

coumarins isolated

from

Fraxinus

chinensis.

Arch

Pharm Res 2007;30(10):1293-301.

Jain PK, Agrawal RK. Antioxidant and free radical scavenging

properties of developed mono-and polyherbal formulations. Asian J

Exp Sci 2008;22(3):213-20.

Lavhale MS, Mishra SH. Evaluation of free radical scavenging activity

of Butea monosperma Lam. Indian J Exp Biol 2007;45(4):376-84.

Pandey M, Sonker K, Kanoujia J, Koshy MK, Saraf SA. Sida

veronicaefolia as a source of natural antioxidant. Int J Pharm Sci Drug

Res 2009;1(3):180-2.

Cuendet M, Hostettmann K, Potterat O. Iridoid glucosides with free

radical scavenging properties from Fagraea blumei. Helvetica Chimi

Acta 1997;80(8):1144-52.

Korycka-Dahl M, Richardson M. Photogeneration of superoxide anion

in serum of bovine milk and in model systems containing riboflavin and

aminoacids. J Dairy Sci 1978;61:400-7.

Pervaiz S, Clement MV. Superoxide anion: Oncogenic reactive oxygen

species? Int J Biochem Cell Biol 2007;39(7-8):1297-304.

Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM,

de Lourdes Basto M. Studies on the antioxidant activity of Lippia

citriodora infusion: Scavenging effect on superoxide radical, hydroxyl

radical and hypochlorous acid. Biol Pharm Bull 2002;25(10):1324-7.

Furuno K, Akasako T, Sugihara N. The contribution of the pyrogallol

moiety to the superoxide radical scavenging activity of flavonoids. Biol

Pharm Bull 2002;25(1):19-23.

Ghiselli A, Nardini M, Baldi A, Scaccini C. Antioxidant activity of

different phenolic fractions separated from an Italian red wine. J Agric

Food Chem 1998;46(2):361-367.

Ou B, Hampsch-Woodill M, Flanagan J, Deemer EK, Prior RL,

Huang D. Novel fluorometric assay for hydroxyl radical prevention

capacity using fluorescein as the probe. J Agric Food Chem

;50(10):2772-7.

Ou B, Hampsch-Woodill M, Prior RL. Development and validation of

an improved oxygen radical absorbance capacity assay using fluorescein

as the fluorescent probe. J Agric Food Chem 2001;49(10):4619-26.

Beretta G, Granata P, Ferrero M, Orioli M, Facino, RM.

Standardization of antioxidant properties of honey by a combination of

spectrophotometric/fluorimetric assays and chemometrics. Anal Chim

Acta 2005;533:185-91.

Soares JR, Dinis TC, Cunha AP, Almeida LM. Antioxidant activities of

some extracts of Thymus zygis. Free Radic Res 1997;26(5):469-78.

Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: Direct

measure of total antioxidant activity of biological fluids and modified

version for simultaneous measurement of total antioxidant power and

ascorbic acid concentration. Methods Enzymol 1999;299:15-27.

Jerkovic I, Marijanovic Z. Oak (Quercus frainetto Ten.) honeydew

honey--approach to screening of volatile organic composition

and antioxidant capacity (DPPH and FRAP assay). Molecules

;15(5):3744-56.

Alvarez-Suarez JM, Tulipani S, Romandini S, Vidaland A, Battino M.

Methodological aspects about determination of phenolic compounds

and in vitro evaluation of antioxidant capacity in the honey. Curr Anal

Chem 2009;5:293-302.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity

assays. J Agric Food Chem 2005;53(6):184-56.

Apak R, Güçlü K, Demirata B, Ozyürek M, Celik SE, Bektasoglu B,

et al. Comparative evaluation of various total antioxidant capacity

assays applied to phenolic compounds with the CUPRAC assay.

Molecules 2007;12(7):1496-547.

Schlesier K, Harwat M, Böhm V, Bitsch R. Assessment of antioxidant

activity by using different in vitro methods. Free Radic Res

;36(2):177-87.

Awika JM, Rooney LW, Wu X, Prior RL, Cisneros-Zevallos L. Screening

methods to measure antioxidant activity of sorghum (Sorghum bicolor)

and sorghum products. J Agric Food Chem 2003;51(23):6657-62.

Badarinath AV, Rao KM, Chetty CM, Ramkanth S, Rajan TV,

Gnanaprakash K. A review on in-vitro antioxidant methods:

Comparisions, correlations and considerations. Int J PharmTech Res

;2(2):1276-85.

Resat A, Kubilay G, Mustafa O, Esin CS. Mechanism of antioxidant

capacity assays and the CUPRAC (cupric ion reducing antioxidant

capacity) assay. Original Paper, Microchim Acta 2008;160:413-9.

Apak R, Güçlü K, Ozyürek M, Bektas Oglu B, Bener M. Cupric ion

reducing antioxidant capacity assay for food antioxidants: Vitamins,

polyphenolics, and flavonoids in food extracts. Methods Mol Biol

;477:163-93.

Apak R, Güçlü K, Ozyürek M, Karademir SE, Altun M. Total

antioxidant capacity assay of human serum using copper(II)neocuproine

as

chromogenic oxidant:

The

CUPRAC

method. Free

Radic

Res 2005;39(9):949-61.

Joana PN, Luis MM, Salette R, Jose LF, Marcela AS. High-throughput

total cupric ion reducing antioxidant capacity of biological samples

determined using flow injection analysis and microplate-based

methods. Anal Sci 2011;27(5):483-8.

Apak R, Guçlu K, Birsen D, Mustafa O, Saliha EC, Burcu B.

Comparative evaluation of various total antioxidant capacity assays

applied to phenolic compounds with the CUPRAC assay: Review.

Molecules 2007;12(7):1496-547.

Apak R, Güçlü K, Ozyürek M, Esin Karademir S, Erçag E. The cupric

ion reducing antioxidant capacity and polyphenolic content of some

herbal teas. Int J Food Sci Nutr 2006;57(5-6):292-304.

Lee G, Rossi MR, Coichev N, Moya HD. The reduction of Cu(II)/

neocuproine complexes by some polyphenols: Total polyphenols

determination in wine samples. Food Chem 2011;26:679-86.

Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in

proteins. J Biol Chem 1927;73:627-50.

Singleton VL, Rudolf O, Lamuela-Raventos RM. Analysis of total

phenols and other oxidation substrates and antioxidants by means of

folin-ciocalteu reagent. Method Enzymol 1999;299:152-78.

Berker KI, Ozdemir Olgun FA, Ozyurt D, Demirata B, Apak R.

Modified Folin-Ciocalteu antioxidant capacity assay for measuring

lipophilic antioxidants. J Agric Food Chem 2013;61(20):4783-91.

Kumar CS, Loh WS, Ooi CW, Quah CK, Fun HK. Structural correlation

of some heterocyclic chalcone analogues and evaluation of their

antioxidant potential. Molecules 2013;18(10):11996-2011.

Oyaizu M. Studies on products of browning reactions-antioxidative

activities of products of browning reaction prepared from glucosamine.

Jpn J Nutr 1986;44:307-15.

Verde V, Fogliano V, Ritieni A, Maiani G, Morisco F, Caporaso N. Use

of N,N-dimethyl-p-phenylenediamine to evaluate the oxidative status

of human plasma. Free Radic Res 2002;36(8):869-73.

Asghar MN, Khan IU, Arshad MN, Sherin L. Evaluation of antioxidant

activity using an improved DMPD radical cation decolourization assay.

Acta Chim Slov 2007;54(2):295.

Nathan CF, Hibbs JB. Role of nitric oxide synthesis in macrophage

antimicrobial activity. Curr Opin Immunol 1991;3(1):65-70.

Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in

health and disease. Physiol Rev 2007;87(1):315-424.

Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antioxidant activities

of methanol extract of Sambucus ebulus L. flower. Pak J Biol Sci

;12:447-50.

Sreejayan N, Rao MN. Nitric oxide scavenging activity by curcuminoids.

J Pharm Pharmacol 2009;49(1):105-7.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide

scavenging property of Ginkgo biloba extract EGB 761. Biochim

Biophys Res Commun 1994;201(2):748-55.

Yagi K. Simple assay for the level of total lipid peroxides in serum or

plasma. Methods Mol Biol 1998;108:101-6.

Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as

diagnostic indices of lipid peroxidation and peroxidative tissue injury.

Free Radic Biol Med 1990;9(6):515-40.

Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V.

Method for the measurement of antioxidant activity in human fluids.

J Clin Pathol 2001;54(5):356-61.

Benzie IF. Lipid peroxidation: A review of causes, consequences,

measurement and dietary influences. Int J Food Sci Nutr

;47(3):233-61.

Cao G, Alessio HM, Cutler RG. Oxygen-radical absorbance capacity

assay for antioxidants. Free Radic Biol Med 1993;14(3):303-11.

Rasmussen CN, Wang XH, Leung S, Andrae-Nightingale LM,

Schmidt SJ, Engeseth NJ. Selection and use of honey as an antioxidant in

a french salad dressing system. J Agric Food Chem 2008;56(18):8650-7.

Gheldof N, Wang XH, Engeseth NJ. Identification and quantification of

antioxidant components of honeys from various floral sources. J Agric

Food Chem 2002;50(21):5870-7.

SAS Institute Inc. SAS Procedures Guide, Version 8. Cary, NC: SAS

Institute Inc.; 1999.

Rice-Evans CA, Miller NJ. Antioxidants-the case for fruit and

vegetables in the diet. Br Food J 1995;97:35-40.

Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice-

Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 14-32

Dontha

Evans C. Polyphenolic flavanols as scavengers of aqueous phase

radicals and as chain-breaking antioxidants. Arch Biochem Biophys

;322(2):339-46.

Rice-Evans CA, Miller NJ, Bolwell GP, Bramley PM, Pridham JB. The

relative antioxidant activities of plant-derived polyphenolic flavonoids.

Free Radic Res 1995;22(4):375-83.

Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity

relationships of flavonoids and phenolic acids. Free Radic Biol Med

;20(7):933-56.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C.

Antioxidant activity applying an improved ABTS radical cation

decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.

Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A.

A novel method for measuring antioxidant capacity and its application

to monitoring the antioxidant status in premature neonates. Clin Sci

(Lond) 1993;84(4):407-12.

Rice-Evans C, Miller NJ. Total antioxidant status in plasma and body

fluids. Methods Enzymol 1994;234:279-93.

Piljac-Zegarac J, Stipcevic T, Belscak A. Antioxidant properties and

phenolic content of different floral origin honeys. J ApiProd ApiMed

Sci 2009;1:43-50.

Rajeshwar Y, Senthil Kumar GP, Malay AG, Mazumder UK. Studies

on in vitro antioxidant ativities of methanol extract of Mucuna pruriens

(Fabaceae) seeds. Eur Bull Drug Res 2005;13:131-8.

Gupta R, Sharma M, Lakshmy R, Prabhakaran D, Reddy KS.

Improved method of total antioxidant assay. Indian J Biochem Biophys

;46(1):126-9.

Bors W, Michel C, Saran M. Inhibition of the bleaching of the

carotenoid crocin: A rapid test for quantifying antioxidant activity.

Biochim Biophys Acta 1984;796:312-9.

Alamdari DH, Ordoudi SA, Nenadis N, Tsimidou MZ, Koliakos G,

Parizadeh SM, et al. Comparison of prooxidant-antioxidant balance

method with crocin method for determination of total prooxidantantioxidant

capacity.

Iran J Basic Med Sci 2006;9(3, 31):158-66.

Manzocco L, Calligaris S, Nicoli MC. Assessment of pro-oxidant

activity of foods by kinetic analysis of crocin bleaching. J Agric Food

Chem 2002;50(10):2767-71

Bors W, Michel C, Saran M. Determination of rate constants for

antioxidant activity and use of the crocin assay. Biochem Clin Appl

;53-63.

Notas G, Miliaraki N, Kampa M, Dimoulios F, Matrella E, Hatzidakis A,

et al. Patients with primary biliary cirrhosis have increased serum total

antioxidant capacity measured with the crocin bleaching assay. World J

Gastroenterol 2005;11(27):4194-8.

Tubaro F, Micossi E, Ursini F. The antioxidant capacity of complex

mixtures by kinetic analysis of crocin bleaching inhibition. J Am Oil

Chem Soc 1996;73:173-9.

Pisoschi AM, Negulescu GP. Methods for total antioxidant activity

determination: A review. Biochem Anal Biochem 2011;1:1-10.

Kunchandy E, Rao MN. Oxygen radical scavenging activity of

curcumin. Int J Pharm 1990;58:237-40.

Esterbauer H, Cheeseman KH. Determination of aldehydic lipid

peroxidation products: Malonaldehyde and 4-hydroxynonenal.

Methods Enzymol 1990;186:407-21.

Okawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues

by thiobarbutiric acid reaction. Anal Biochem 1999;95(2):351-8.

Zhang XY. Principles of Chemical Analysis. Beijing: Chine Science

Press; 2000.

Winston GW, Regoli F, Dugas AJ Jr, Fong JH, Blanchard KA. A rapid

gas chromatographic assay for determining oxyradical scavenging

capacity of antioxidants and biological fluids. Free Radic Biol Med

;24(3):480-93.

Regoli F, Winston GW. Quantification of total oxidant scavenging

capacity of antioxidants for peroxynitrite, peroxyl radicals, and

hydroxyl radicals. Toxicol Appl Pharmacol 1999;156(2):96-105.

Lichtenthäler R, Marx F. Total oxidant scavenging capacities of

common European fruit and vegetable juices. J Agric Food Chem

;53(1):103-10.

Popov I, Lewin G. Antioxidative homeostasis: Characterization

by means of chemiluminescent technique. Methods Enzymol

;300:437-56.

Jimenez AM, Navas MJ. Chemiluminescence methods (present and

future). Grasas Aceites 2002;53:64-75.

Jayaprakasha GK, Singh RP, Sakariah KK. Antioxidant activity of

grape seed (Vitis vinifera) extracts on peroxidation modls in vitro. Food

Chem 2001;73:288-90.

Miller HE. A simplified method for the evaluation of anti-oxidants. J

Am Oil Chem Soc 1971;18:439-52.

Lee HS, Coates GA. Measurement of total vitamin C activity in citrus

products by HPLC. J Liq Chromatogr Relat Technol 1999;22:2367-87.

Gonzalez E, Vaillant F, Rojas G, Pérez A. Novel semiautomated

method for assessing in vitro cellular antioxidant activity using the

light scattering properties of human erythrocytes. J Agric Food Chem

;58(3):1455-61.

Gonzalez E, Vaillant F, Pérez AM, Rojas G. In vitro cell-mediated

antioxidant protection of human erythrocytes by some common tropical

fruits. J Nutr Food Sci 2012;2(139):1-8.

Harbour JR, Chow V, Bolton JR. An electron spin resonance study of

the spin adducts of OH and HO

radicals with nitrones in the ultraviolet

photolysis of aqueous hydrogen peroxide solutions. Can J Chem

;52:3549-53.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantification of

antioxidant capacity through the formation of a phosphomolybdenum

complex: Specific application to the determination of vitamin E. Anal

Biochem 1999;269(2):337-41.

Sudha G, Priya MS, Shree RI, Vadivukkarasi S. In vitro free radical

scavenging activity of raw pepino fruit (Solanum muricatum Aiton). Int

J Curr Pharm Res 2011;3(2):137-40.

Shi X, Dalal NS, Jain AC. Antioxidant behaviour of caffeine: Efficient

scavenging of hydroxyl radicals. Food Chem Toxicol 1991;29(1):1-6.

Weil JA, Bolton JR, Wertz JE. Electron Paramagnetic Resonance:

Elementary Theory and Practical Applications. New York: John Wiley

& Sons; 1994.

Duling DR. Simulation of multiple isotropic spin-trap EPR spectra.

J Magn Reson B 1994;104(2):105-10.

Mombourquette MJ, Weil JA. Fortran Program EPR NMR. Version

4. Saskatoon: Department of Chemistry, University of Saskatchewan;

Noro T, Oda Y, Miyase T, Ueno A, Fukushima S. Inhibitors of xanthine

oxidase from the flowers and buds of Daphne genkwa. Chem Pharm

Bull (Tokyo) 1983;31(11):3984-7.

Soler-Rivas C, Espin JC, Wichers HJ. An easy and fast test to compare

total free radical scavenger capacity of foodstuffs. Phytochem Anal

;11(5):330-8.

Dinis TC, Maderia VM, Almeida LM. Action of phenolic derivatives

(acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of

membrane lipid peroxidation and as peroxyl radical scavengers. Arch

Biochem Biophys 1994;315(1):161-9.

Jagtap UB, Panaskar SN, Bapat VA. Evaluation of antioxidant capacity

and phenol content in jackfruit (Artocarpus heterophyllus Lam.) fruit

pulp. Plant Foods Hum Nutr 2010;65(2):99-104.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as

a measure of “antioxidant power”: The FRAP assay. Anal Biochem

;239(1):70-6.

Sapakal VD, Shikalgar TS, Ghadge RV, Adnaik RS, Naikwade NS,

Magdum CS. In vivo screening of antioxidant profile: A review. J Herb

Med Toxicol 2008;2(2):1-8.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys

;82(1):70-7.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative

characterization of erythrocyte glutathione peroxidase. J Lab Clin Med

;70(1):158-69.

Yang GQ, Chen JS, Wen ZM, Ge KY, Zhu LZ, Chen XC, et al. The role

of selenium in keshan disease. Adv Nutr Res 1984;6:203-31.

Wood JL. In: Fishman WH. editor. Metabolic Conjugation and

Metabolic Hydrolysis. Vol. II. New York: Academic Press; 1970.

p. 61-299.

Jocelyn PC. Biochemistry of the SH Group. London: Academic Press;

p. 10.

McCord JM, Fridovich I. Superoxide dismutase. An enzymic function

for erythrocuprin (hemocuprein). J Biol Chem 1969;244(22):6049-55.

Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.

Singhal PK, Kapur N, Dhillon KS, Beamish RE, Dhalla NS. Role of free

radicals in catecholamine cardiomyopathy. Can J Physiol Pharmacol

;60(11):1390-7.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay

of superoxide dismutase. Indian J Biochem Biophys 1984;21(2):131-2.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues

by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.

Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol

;52:302-10.

el-Saadani M, Esterbauer H, el-Sayed M, Goher M, Nassar AY,

Jürgens G. A spectrophotometric assay for lipid peroxides in serum

lipoproteins using a commercially available reagent. J Lipid Res

Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 14-32

Dontha

;30(4):627-30.

Liu RH, Finley J. Potential cell culture models for antioxidant research.

J Agric Food Chem 2005;53(10):4311-4.

Wolfe KL, Liu RH. Cellular antioxidant activity (CAA) assay for

assessing antioxidants, foods, and dietary supplements. J Agric Food

Chem 2007;55(22):8896-907.




About this article

Title

A REVIEW ON ANTIOXIDANT METHODS

DOI

10.22159/ajpcr.2016.v9s2.13092

Date

01-10-2016

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 9 Suppl 2 October 2016 Page: 14-32

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

347 Views | 376 Downloads

Authors & Affiliations

Dontha Sunitha
Assistant Professor, Malla Reddy College of Pharmacy.
India


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