PHYTOCHEMICAL STUDIES ON THE GLYCOSIDES OF LEAF EXTRACTS OF MEDICINALLY IMPORTANT PLANT Holoptelea Integrifolia (Roxb.) PLANCH USING HPTLC
Objective: This study was conducted to identify the glycosides from petroleum ether and methanol extracts of medicinally and economically useful
leaves of Holoptelea integrifolia (Roxb.) planch using high-performance thin layer chromatography (HPTLC) technique.
Materials and Methods: Preliminary phytochemical screening was carried out and HPTLC studies were carried out. Camag HPTLC system equipped
with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at
540 nm and operated by Win computer-aided transcription system software (1.4.6 Camag) with the help of tungstant lamp.
Results: Preliminary phytochemical screening of petroleum extract of H. integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides,
flavonoids, proteins, tannins and carbohydrates, while methanolic extract of H. integrifolia showed the presence of steroids, alkaloids, flavonoids,
proteins, and carbohydrates. HPTLC fingerprinting of glycosides of petroleum ether extract of leaf revealed four polyvalent phytoconstituents
(4 peaks) and corresponding ascending order of radio frequency (Rf) values in the range of 0.37-0.82. while methanol extract of leaf showed ten
polyvalent phytoconstituents (10 peaks) and corresponding ascending order of Rf values in the range of 0.20-0.83.
Conclusions: With the above Rf values and preliminary phytochemical analysis we have concluded the presence of glycosides in both the extracts.
Keywords: Holoptelea integrifolia (Roxb.) planch leaf, Phytochemical screening, Glycosides, High performance thin layer chromatography
Nadkarni KM. Indian Materia Medica. Mumbai, India: Popular
Prakashan Pvt. Ltd.; 1976. p. 651-2.
Kirtikar KR, Basu BD. Indian Medicinal Plants. 3rd ed., Vol. III.
New Delhi, India: Sri Satguru Publications; 2000. p. 2292-4.
Prajapati ND, Purohit SS, Sharma AK. AHandbook of Medicinal Plants
a Complete Source Book. Jodhpur, India: Agrobias; 2003. p. 273.
Warrier PK, Nambiar VP, Ramakutty C. Indian Medicinal Plants a
Compendium of 500 Species. Vol. 3. Hyderabad: Orient Longman
Private Limited; 1995. p. 162.
Kalpana, Upadhyay A. Anti-inflammatory evaluation of
ethanolic extract of leaves of Holoptelea integrifolia, Planch.
Annal Biol Res 2010;1(2):185-95.
Sharma S, Khatri P, Pandey A, Jakhetia V, Chaturvedi L, Dwivedi N.
Anti-diabetic screening of leaves extract of Holoptelea integrifolia. Int
J Pharm Res Dev 2010;2(10):66-71.
Lakshmi KS, Sharma SS, Rajesh T, Chitra V. Antitumour activity of
Holoptelia integrifolia on Daltons ascetic lymphoma in Swiss albino
mice. Int J Green Pharm 2010;4(1):44-47.
Shrinivas S, Lakshmi KS, Rajesh T. Evaluation of antidiarrhoeal
potentials of ethanolic extract of leaves of Holoptelea integrifolia in
mice model. Int J PharmTech Res 2009;1(3):832-6.
Saraswathy A, Devi SN, Ramasamy D. Antioxidant, heavy metals and
elemental analysis of Holoptelea integrifolia Planch. Indian J Pharm
Paarakh PM, Nadella D. Antibacterial activity of different extracts
of stem bark of Holoptelea integrifolia Roxb. Int Res J Pharm
Durga N, Paarakh PM. Evaluation of anthelmintic activity of stem bark
of Holoptelea integrifolia Planch. Int J Res Ayur Pharm 2010;1:637-41.
Kumar B, Kaur S, Puri S, Tiwari P, Divakar K. Comparative study
of anthelmintic activity of aqueous and ethanolic extract of bark of
Holoptelea integrifolia. Int J Drug Dev Res 2010;2(4):758-63.
Khandelwal KR. Practical Pharmacognosy. Techniques and
Experiments. 10th ed. Pune, India: Nirali Prakashan; 2006. p. 149-56.
Harborne JB. Phytochemical Methods. 3rd ed. London: Chapman and
Wagner H, Baldt S. Plant Drug Analysis. Berlin: Springer;
Scott RP. Encyclopedia of Chromatography. 10th ed.
USA: Marcel Dekker; 2001. p. 252-4.
ICH/CPMP Guidelines Q2B, Validation of Analytical Procedures
â€“ Methodology, 1996
Cazes J, Scott RP. Chromatography Theory. NY: Marcel Decker; 2002.
Reviewer Guidance, Validation of Chromatographic
Methods. Center for Drug Evaluation and Research, US Food
and Drug Administration; 1994.
Sethi PD. HPTLC: Quantitative Analysis of Pharmaceutical
Formulations. New Delhi: CBS Publications; 1996. p. 162-5.
Heftman E. Chromatography Fundamentals and Applications of
Chromatography and Related Differential Migration Methods. 6th ed.,
Vol. 69A. Amsterdam: Elsevier; 2004. p. 253-91.
British Pharmacopoeia. International ed., Vol. II. Cambridge: HMSO;
Appendix 112 (IB).
Sherma J. Encyclopedia of Pharmaceutical Technology. 2nd ed. USA:
Marcel Dekker; 2001. p. 252-4.
ICH/CPMP Guidelines Q2A, Text on Validation of
Analytical Procedures; 1994
???. USP 23, NF 19, Asian ed. Rockville, M.D: United States
Pharmacopeial Convention; 1982. p. 1225.
Hariprasad PS, Ramakrishnan N. HPTLC fingerprint profile of Rumex
Vesicarius L. Asian J Pharm Clin Res 2011;4(2):134-6.
Swarnalatha Y, Kommineni L. Finger printing analysis of the alkaloids
from Sphaeranthus amaranthoides leaves using HPTLC analysis.
Asian J Pharm Clin Res 2014;7(2):125-7.
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