• RAVINDRA C SUTAR sanjivani college of pharmacy, kopargaon, Maharashtra, India.
  • SANJAY B KASTURE sanjivani college of pharmacy,kopargaon, Maharashtra, India.
  • KALAICHELVAN VK Dept.of Pharmacy,Annamalai University,TamilNadu, India.


Objective: The present study was conducted to identify the phytosterols from petroleum ether and methanol extracts of medicinally and economically
useful leaves of Holoptelea integrifolia (Roxb.) Planch using high-performance thin layer chromatography (HPTLC) technique.
Materials and Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. Camag HPTLC system equipped with
Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag thin layer chromatography scanner IV in the reflectance
absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp.
Results: Preliminary phytochemical screening of petroleum ether extract of H. integrifolia showed the presence of steroids, terpenoids, alkaloids,
glycosides, flavonoids, proteins, tannins, and carbohydrates while methanolic extract of H. integrifolia showed the presence of steroids, alkaloids,
flavonoids, proteins, and carbohydrates. HPTLC fingerprinting of phytosterols of petroleum ether extract of leaf revealed five polyvalent
phytoconstituents (5 peaks) and corresponding ascending order of Rf values in the range of 0.11-0.45. While methanol extract of leaf showed four
polyvalent phytoconstituents (4 peaks) and corresponding ascending order of Rf values in the range of 0.13-0.48.
Conclusions: With the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phytosterols in both
the extracts.

Keywords: High-performance thin layer chromatography fingerprinting, Holoptelea integrifolia (Roxb.) planch leaf, Phytochemical screening,

Author Biographies

RAVINDRA C SUTAR, sanjivani college of pharmacy, kopargaon, Maharashtra, India.



SANJAY B KASTURE, sanjivani college of pharmacy,kopargaon, Maharashtra, India.


KALAICHELVAN VK, Dept.of Pharmacy,Annamalai University,TamilNadu, India.

Asst. Prof.


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How to Cite
SUTAR, R. C., S. B. KASTURE, and K. VK. “FINGER PRINTING ANALYSIS OF THE PHYTOSTEROLS FROM Holoptelea Integrifolia (Roxb.) PLANCH LEAVES USING HPTLC ANALYSIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 7, no. 4, Sept. 2014, pp. 160-4,
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