• Astridani Rizky Putranti Airlangga University
  • Riesta Primaharinastiti
  • Esti Hendradi




Objective: The aim of this study was to investigate physical characteristics of nanostructured lipid carriers (NLCs) mixture of alpha tocopheryl acetate, cetyl palmitate, Tween 80 and propylene glycol using high shear homogenization technique on NLC preparation to predict the optimum ratio of alpha tocopheryl acetate-cetyl palmitate to produce good characteristics of NLC loaded coenzyme, higher % EE, good penetration, controlled release, and stable.

Methods: Lipid characterizations were conducted by diffraction scanning calorimetry, X-ray diffraction, and Fourier transforms infrared spectrophotometry. Coenzyme Q10 concentration was measured by spectrophotometer at 275 nm. NLC characteristics based on their morphology was determined using transmission electron microscope, particle size, and its polydispersity index which were measured with Delsa Nanoâ„¢ particle size analyzer. Percentage of coenzyme Q10 entrapped in NLC was determined by dialysis bag method. Coenzyme Q10 release profile was measured using with Franz cell for 12 hrs. The penetration depth of NLC coenzyme Q10 in abdominal skin of Wistar rat was determined with fluorescence microscopy using rhodamine B as marker. NLC physical stability based on minimum of particle size variation, pH and viscosity during 90 days storage.

Results: The result showed that formula with ratio of cetyl palmitate-alpha tocopheryl acetate 70:30 (% w/w) produce good characteristics of NLC

loaded coenzyme, higher % EE, good penetration, controlled release, and stable in 90 days storage.

Conclusion: The coenzyme Q10 NLC system with cetyl palmitate and alpha tocopherol acetate as lipid matrixare characterized by small particle size, low crystallinity, spherical morphology of particle and high coenzyme Q10 entrapment efficiency. Crystal modification led to the formation of a more amorphous thereby increasing the drug entrapment

Keywords: Coenzyme Q10, Nanostructured lipid carrier, Cetyl palmitate, Alpha tocopheryl acetate, High shear homogenization.


Download data is not yet available.

Author Biography

Astridani Rizky Putranti, Airlangga University



Hargreaves IP. Coenzyme Q10 as a therapy for mitochondrial disease.

Int J Biochem Cell Biol 2014;49:105-11.

Kovacevic A, Savic S, Vuleta G, Müller RH, Keck CM. Polyhydroxy

surfactants for the formulation of lipid nanoparticles (SLN and NLC):

Effects on size, physical stability and particle matrix structure. Int J Pharm 2011;406(1-2):163-72.

López-García R, Ganem-Rondero A. Solid lipid nanoparticles (SLN) and

nanostructured lipid carriers (NLC): Occlusive effect and penetration

enhancement ability. J Cosmet Dermatol Sci Appl 2015;5:62-72.

Loo CH, Basri M, Ismail R, Lau HL, Tejo BA, Kanthimathi MS, et al.

Effect of compositions in nanostructured lipid carriers (NLC) on skin

hydration and occlusion. Int J Nanomedicine 2013;8:13-22.

Brugè F, Damiani E, Puglia C, Offerta A, Armeni T, Littarru GP,

et al. Nanostructured lipid carriers loaded with Coq10: Effect on

human dermal fibroblasts under normal and UVA-mediated oxidative

conditions. Int J Pharm 2013;455(1-2):348-56.

Fan H, Liu G, Huang Y, Li Y, Xia Q. Development of a nanostructured

lipid carrier formulation for increasing photo-stability and water

solubility of phenylethyl resorcinol. Appl Surf Sci 2014;288:193-200.

Agmo Hernández V, Eriksson EK, Edwards K. Ubiquinone-10 alters

mechanical properties and increases stability of 2 phospholipid

membranes. Biochim Biophys Acta 2015;1848:2233-43.

Pardeike J, Schwabe K, Müller RH. Influence of nanostructured lipid

carriers (NLC) on the physical properties of the Cutanova Nanorepair

Q10 cream and the in vivo skin hydration effect. Int J Pharm


Almeida MM, Bou-Chacra NA, Conte JD, Kaneko TM,

Baby AR, Velasco MV. Evaluation of physical and chemical stability

of nanostructured lipid carries containing ursolic acid in cosmetic

formulation. J Appl Pharm Sci 2013;3(01):005-8.

Castelli F, Puglia C, Sarpietro MG, Rizza L, Bonina F. Characterization

of indomethacin-loaded lipid nanoparticles by differential scanning

calorimetry. Int J Pharm 2005;304(1-2):231-8.

Piao H, Ouyang M, Xia D, Quan P, Xiao W, Song Y, et al. In vitro–in

vivo study of CoQ10-loaded lipid nanoparticles in comparison with

nanocrystals. Int J Pharm 2011;419(1-2):255-9.

Teeranachaideekul V, Souto EB, Junyaprasert VB, Muller RH.

Cetyl palmitate-based NLC for topical delivery of Coenzyme Q10 –

Development, physicochemical characterization and in vitro release

studies. Eur J Pharm Biopharm 2007;67(1):141-8.

Gupta RB. Fundamentals of Drug Nanoparticles in Nanoparticle

Technology for Drug Delivery. Vol. 159. New York: Taylor and Francis;

p. 124-30.

Lucangioli S, Tripodi V. The importance of the formulation in the

effectiveness of coenzyme Q10 supplementation in mitochondrial

disease therapy. Der Pharm Sin 2012;3(4):406-7.

Teeranachaideekul V, Boonme P, Souto EB, Müller RH,

Junyaprasert VB. Influence of oil content on physicochemical

properties and skin distribution of Nile red-loaded NLC. J Control

Release 2008;128(2):134-41.

Yue Y, Zhou H, Liu G, Li Y, Yan Z, Duan M. The advantages of a

novel CoQ10 delivery system in skin photo-protection. Int J Pharm


Zhai Y, Xiaoye Y, Zhao L, Wang Z, Zhai G. Lipid nanocapsules for

transdermal delivery of ropivacaine: In vitro and in vivo evaluation. Int

J Pharm 2014;471(1-2):103-11.

Korkm E, Gokce EH, Ozer O. Development and evaluation of

coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced

dermal delivery. Acta Pharm 2013;63(4):517-29.



How to Cite

Putranti, A. R., R. Primaharinastiti, and E. Hendradi. “EFFECTIVITY AND PHYSICOCHEMICAL STABILITY OF NANOSTRUCTURED LIPID CARRIER COENZYME Q10 IN DIFFERENT RATIO of LIPID ALFA CETYL PALMITATE AND ALPHA TOCOPHERYL ACETATE AS CARRIER”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 2, Feb. 2017, pp. 146-52, doi:10.22159/ajpcr.2017.v10i2.14835.



Original Article(s)