EVALUATION OF EX VIVO ANTICATARACT ACTIVITY OF ETHANOLIC EXTRACT OF ALSTONIA SCHOLARIS LEAVES ON DEXAMETHASONE-INDUCED CATARACT BY USING ISOLATED GOAT LENS
Objective: To investigate the ex vivo anticataract activity of ethanolic extract of Alstonia scholaris (EEAS) leaves on dexamethasone-induced cataract using isolated goat lens.
Methods: Anticataract activity is done using isolated goat lens. Goat lens was divided into four groups. Group I: Lens was incubated in artificial aqueous humor (normal control). Group II: Lens was incubated with dexamethasone 10 mg (toxic control). Group III and IV: Lens was incubated with dexamethasone and EEAS (50 Âµg and 100 Âµg) and subjected to photographic evaluation for opacity; lens was homogenized using Tris-phosphate buffer, and sodium, potassium, total protein, and catalase concentrations were determined.
Results: The grades of opacity were 0, 3, 1, and 1 in group I, II, III, and IV, respectively. The present study showed higher total proteins (p<0.05 at all concentration) and K+Â ions (p<0.05 at all concentration), whereas lower concentrations of Na+Â ions (p<0.05 at all concentration) with EEAS-treated groups. The level of catalase was found to be less in experimentally induced cataract lenses as compared to normal control group. The lenses treated with EEAS showed a significant rise in enzyme level suggesting maintenance of antioxidant enzyme integrity.
Conclusion: The present investigation suggests that EEAS leaves effectively prevent the cataractogenic condition. Thus, the goat lens model and dexamethasone-induced cataract model could be used for testing of various anticataract agents.
Keywords: Cataract, Artificial aqueous humor, Lens, Dexamethasone, Alstonia scholaris.
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