EVALUATION OF EX VIVO ANTICATARACT ACTIVITY OF ETHANOLIC EXTRACT OF ALSTONIA SCHOLARIS LEAVES ON DEXAMETHASONE-INDUCED CATARACT BY USING ISOLATED GOAT LENS

  • Nagaraju Bandaru ACHARYA NAGARAJUNA UNIVERSITY
  • Ramu A
  • Vijetha P
  • Vidhyadhara S
  • Nandini P
  • Abhilash Reddy S

Abstract

Objective: To investigate the ex vivo anticataract activity of ethanolic extract of Alstonia scholaris (EEAS) leaves on dexamethasone-induced cataract using isolated goat lens.

Methods: Anticataract activity is done using isolated goat lens. Goat lens was divided into four groups. Group I: Lens was incubated in artificial aqueous humor (normal control). Group II: Lens was incubated with dexamethasone 10 mg (toxic control). Group III and IV: Lens was incubated with dexamethasone and EEAS (50 µg and 100 µg) and subjected to photographic evaluation for opacity; lens was homogenized using Tris-phosphate buffer, and sodium, potassium, total protein, and catalase concentrations were determined.

Results: The grades of opacity were 0, 3, 1, and 1 in group I, II, III, and IV, respectively. The present study showed higher total proteins (p<0.05 at all concentration) and K+  ions (p<0.05 at all concentration), whereas lower concentrations of Na+  ions (p<0.05 at all concentration) with EEAS-treated groups. The level of catalase was found to be less in experimentally induced cataract lenses as compared to normal control group. The lenses treated with EEAS showed a significant rise in enzyme level suggesting maintenance of antioxidant enzyme integrity.

Conclusion: The present investigation suggests that EEAS leaves effectively prevent the cataractogenic condition. Thus, the goat lens model and dexamethasone-induced cataract model could be used for testing of various anticataract agents.

Keywords: Cataract, Artificial aqueous humor, Lens, Dexamethasone, Alstonia scholaris.

 

Author Biography

Nagaraju Bandaru, ACHARYA NAGARAJUNA UNIVERSITY
PHARMACOLOGY

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How to Cite
Bandaru, N., R. A, V. P, V. S, N. P, and A. R. S. “EVALUATION OF EX VIVO ANTICATARACT ACTIVITY OF ETHANOLIC EXTRACT OF ALSTONIA SCHOLARIS LEAVES ON DEXAMETHASONE-INDUCED CATARACT BY USING ISOLATED GOAT LENS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 182-5, doi:10.22159/ajpcr.2017.v10i2.15084.
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