PHOSPHOLIPASE A 2 PLASMA ELASTASE ACTIVITY IN PRE AND POST PARTUM OF PRE-ECLAMPTIC WOMEN
Objective: The objectives of the present study were to evaluate the activity of phospholipase A 2 , plasma elastase enzymes and to assess relation with
an inflammatory marker high sensitive C-reactive protein (hs-CRP) in nonpregnant before and after delivery of normotensive pregnant and preeclamptic
Methods: The study population consists of three groups: Nonpregnant (Group 1, n=57), normotensive pregnant (Group 2, n=57), and pre-eclamptic
women (Group 3, n=57). Groups 2 and 3 were followed after delivery within 48 hrs. Phospholipase A , plasma elastase, and hs-CRP levels were determined spectrophotometrically.
Results: The plasma elastase, phospholipase A 22 activity, and hs-CRP were elevated in pre-eclampsia significantly (p<0.05), nonsignificant rise in
normotensive pregnant before delivery condition compared to nonpregnant women. However, plasma elastase in normal pregnancy and pre-eclampsia
were decreased by 1.2- and 2.07-fold, respectively, after delivery. Whereas phospholipase A and hs-CRP found to be nonsignificantly decreased in the
postdelivery status of the both the groups. Receiver operating characteristics curve analysis showed that elastase enzyme has diagnostic importance to assess inflammation on the basis of area under curve (0.758).2
Conclusion: Our research findings generated knowledge about raised level of plasma elastase enzyme by neutrophil degranulation represents inflammation in pre-eclampsia. Elevated elastase, phospholipase A
Keywords: Elastase, High sensitive C - reactive protein, Phospholipase A2
with hs-CRP in pre-eclampsia serves as indicators of inflammation., Pre-eclampsia.
Objective:Theobjectivesofthepresentstudywere toevaluatetheactivityofphospholipaseA,plasmaelastaseenzymesand toassessrelationwith an inflammatorymarkerhighsensitiveC-reactiveprotein(hs-CRP)innonpregnantbeforeandafterdeliveryofnormotensivepregnantandpre- eclamptic women.
weredecreasedby1.2-and2.07-fold,respectively,afterdelivery.WhereasphospholipaseA Â andhs-CRPfoundtobenonsignificantlydecreasedinthe postdelivery status of the both the groups. Receiver operating characteristics curve analysis showed that elastase enzyme has diagnostic importance to assess inflammation on the basis of area under curve (0.758).
Conclusion: Ourresearchfindingsgeneratedknowledgeaboutraisedlevelofplasmaelastaseenzymebyneutrophildegranulationrepresents inflammation in pre-eclampsia. Elevated elastase, phospholipase A Â with hs-CRP in pre-eclampsia serves as indicators of inflammation.
2Â Keywords:Elastase, High sensitive C - reactive protein, Phospholipase A, Pre-eclampsia.
2. Roberts JM, Redman CW. Pre-eclampsia: More than pregnancy- induced hypertension. Lancet 1993;341(8858):1447-51.
3. Sibai BM. Hypertensive disorders of pregnancy: The United States perspective. Curr Opin Obstet Gynecol 2008;20(2):102-6.
4. Cindrova-Davies T. Gabor than award lecture 2008: Pre- eclampsia - From placental oxidative stress to maternal endothelial dysfunction. Placenta 2009;30 Suppl A: S55-65.
5. Borzychowski AM, Sargent IL, Redman CW. Inflammation and pre-eclampsia. Semin Fetal Neonatal Med 2006;11(5):309-16.
6. Lok CA, Jebbink J, Nieuwland R, Faas MM, Boer K, Sturk A, et al. Leukocyte activation and circulating leukocyte-derived microparticles in preeclampsia. Am J Reprod Immunol 2009;61(5):346-59.
7. Catarino C, Rebelo I, Belo L, Rocha-Pereira P, Rocha S, Castro EB, et al. Fetal lipoprotein changes in pre-eclampsia. Acta Obstet Gynecol Scand 2008;87(6):628-34.
8. Kavitha V, Srinivas B, Mvln PK, Shashikanth P. Assessment of placental oxidative stress parameters in pre-eclamptic and normal pregnant women. Asian J Pharm Clin Res 2016;9(1):105-9.
9. Korkmaz B, Horwitz MS, Jenne DE, Gauthier F. Neutrophil elastase, proteinase 3, and cathepsin G as therapeutic targets in human diseases. Pharmacol Rev 2010;62(4):726-59.
10. Dennis EA. Diversity of group types, regulation, and function of phospholipase A2. J Biol Chem 1994;269(18):13057-60.
11. Macy EM, Hayes TE, Tracy RP. Variability in the measurement of C-reactive protein in healthy subjects: Implications for reference intervals and epidemiological applications. Clin Chem 1997;43(1):52-8.
12. Bambrana V, Dayanand CD, Sheela R. Evaluation of lipid peroxidation, protein carbonyl content and total antioxidant status in pre and post- delivery of women with pre-eclampsia. Am J Pharm Health Res 2014;2:5-99.
13. Bambrana V, Dayanand CD, Kotur PP. Is xanthine oxidase, a marker in preeclampsia? A case-control study. J Clin Diagn Res 2015;9(10):1-3.
14. Bambrana V, Dayanand CD. Kotur P. Relationship between xanthine oxidase, ischemia modified albumin, nitric oxide with antioxidants in non pregnant, pre and post-delivery of normal pregnant and preeclampsia. Indian J Clin Biochem 2016. DOI: 10.1007/s12291-016-0599-0.
15. Bieth J, Spiess B, Wermuth CG. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochem Med 1974;11(4):350-7.
16. Price JA 3rd. A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH. J Biochem Biophys Methods 2007;70(3):441-4.
17. Redman CW, Sargent IL. Preeclampsia and the systemic inflammatory response. Semin Nephrol 2004;24(6):565-70.
18. Udenze I, Amadi C, Awolola N, Makwe CC. The role of cytokines as inflammatory mediators in preeclampsia. Pan Afr Med J 2015;20:219.
19. Grommes J, Soehnlein O. Contribution of neutrophils to acute lung injury. Mol Med 2011;17(3-4):293-307.
20. Catarino C, Santos-Silva A, Belo L, Rocha-Pereira P, Rocha S, PatrÃcio B, et al. Inflammatory disturbances in preeclampsia: Relationship between maternal and umbilical cord blood. J Pregnancy 2012;2012:684384.
21. Laresgoiti-Servitje E. A leading role for the immune system in the pathophysiology of preeclampsia. J Leukoc Biol 2013;94(2):247-57.
22. von Versen-Hoeynck FM, Hubel CA, Gallaher MJ, Gammill HS, Powers RW. Plasma levels of inflammatory markers neopterin, sialic acid, and C-reactive protein in pregnancy and preeclampsia. Am J Hypertens 2009;22(6):687-92.
23. Belo L, Santos-Silva A, Caslake M, Cooney J, Pereira-Leite L, Quintanilha A, et al. Neutrophil activation and C-reactive protein concentration in preeclampsia. Hypertens Pregnancy 2003;22(2):129-41.
24. Gupta AK, Gebhardt S, Hillermann R, Holzgreve W, Hahn S. Analysis of plasma elastase levels in early and late onset preeclampsia. Arch Gynecol Obstet 2006;273(4):239-42.
25. Cunze T, Osmers R, Herzog S, Speer C, Kuhn W. Changes in plasma elastase during pregnancy and sub partu. Gynecol Obstet Invest 1998;45(2):89-92.
26. Pulkkinen MO, Poranen AK, Kivikoski AI, Nevalainen TJ. Elevated serum group II phospholipase A2 levels are associated with decreased blood flow velocity in the umbilical artery. Gynecol Obstet Invest 1996;41(2):93-5.
27. Seki H, Takeda S, Kinoshita K, Satoh K. Activities of phospholipase A2, cyclooxygenase, and PGI2 synthase of umbilical venous endothelial cells in preeclamptic women. Asia Oceania J Obstet Gynaecol 1994;20(4):419-25.
28. Zhou Y, Niu J, Duan D, Lei Q, Wen J, Lin X, et al. Lipoprotein-associated phospholipase A2 is associated with postpartum hypertension in women with history of preeclampsia. Heart Vessels 2015;30(4):503-9.
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