EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY BY FLAVONOIDS FROM PONGAMIA PINNATA LINN

  • Vanishree Bambrana Sri Devaraj Urs medical college, SDUAHER,Kolar
  • Dayanand Cd Sri Devaraj Urs medical college, SDUAHER,Kolar
  • Sheela Sr Sri Devaraj Urs medical college, SDUAHER,Kolar

Abstract

ABSTRACT
Objective: Flavonoids from the crude seeds extract of Pongamia pinnata L., dried fruit powder of Morinda citrifolia L., bark of Mangifera indica L., and
rhizome of Zingiber officinale Rosc. were screened for xanthine oxidase (XO) inhibition at different concentration. The inhibitory potential of quercetin
and allopurinol were used for the determination of 50% inhibitory concentration (IC50) and Ki values.
Methods: Isolation of flavonoids from the plant extracts was processed by column chromatography and tested for XO inhibitory activity in the range
of 6-800 μg/ml.
Results: The results demonstrated that optimized flavonoids extract of P. pinnata L. exhibited promising XO inhibition. P. pinnata L., M. indica L., and
Z. officinale Rosc. had IC50 in the concentration of 8.74 mM, 1.09 mM, 5.4 mM and Ki 0.35 mM, 1.73 mM, 2.7 mM, respectively.
Conclusion: The study showed that plant species under investigation exhibited XO inhibition by optimized flavonoid extract. P. pinnata L. indicated
promising XO inhibition compared to other plant extracts. Flavonoids can be used as a potent inhibitor of XO an alternative to allopurinol.
Keywords: Xanthine oxidase, Quercetin, Allopurinol, Pongamia pinnata, Oxidative stress.

Author Biographies

Vanishree Bambrana, Sri Devaraj Urs medical college, SDUAHER,Kolar
Ph.D. Scholar Department of Biochemistry
Dayanand Cd, Sri Devaraj Urs medical college, SDUAHER,Kolar

Professor

Department of Biochemistry

Sheela Sr, Sri Devaraj Urs medical college, SDUAHER,Kolar

Professor

Department of Obsterics & Gynecology

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Bambrana, V., D. Cd, and S. Sr. “EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY BY FLAVONOIDS FROM PONGAMIA PINNATA LINN”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 3, Mar. 2017, pp. 360-2, doi:10.22159/ajpcr.2017.v10i3.16303.
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