IN VITRO CYTOTOXIC AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES ON ORTHOSIPHON STAMINEUS BENTH. (LEAF) AGAINST MCF–7 CELL LINES


Renuka Saravanan, Brindha Pemiah, Mahesh Narayanan, Sivakumar Ramalingam

Abstract


ABSTRACT
Objective: The objective of the study was to determine the anticancer efficacy of Orthosiphon stamineus extract against Michigan Cancer Foundation-7
(MCF-7) and its phytochemical analysis through gas chromatography-mass spectrometry (GC-MS).
Methods: Different solvents were used for leaf extraction and used for qualitative assay of phytochemicals using standard protocols. Different
concentration (12.5, 25, 50, 100, and 200 μg/ml) of methanol extract and ethyl acetate extract of O. stamineus leaves were used to assess the in vitro
cytotoxic effect using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the ethyl acetate extract was subjected to
GC-MS analysis, and the identification of components was based on the National Institute of Standards and Technology library database.
Result: Of the hexane, methanol and ethyl acetate extracts, methanol extract found to contain more of phytochemicals followed by ethyl acetate. The
inhibitory concentration 50 for methanol extract and ethyl acetate extract was found be 93.42 μg/ml and 215.3 μg/ml, respectively.
GC-MS mass spectrum of ethyl acetate extract revealed the presence of squalene and phytol and antioxidants such as flavones. Sterols compounds
such as vitamin E, ergosterol, cholesterol, and 3,7,11,15-tetramethyl-2-hexadecen-1-ol and terpenoids were also identified.
Conclusion: The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the results
further depicted that the selected traditional anti-cancer plants could be used not only as an anti-cancer but also as a good antioxidant.
Keywords: Anti-cancer, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Orthosiphon stamineus, Michigan cancer fsoundation-7
cells.


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References


REFERENCES

Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life

Sci 2005;78(5):431-41.

Jones WP, Chin YW, Kinghorn AD. The role of pharmacognosy in

modern medicine and pharmacy. Curr Drug Targets 2006;7(3):247-64.

Kim J. Protective effects of Asian dietary items on cancers - Soy and

ginseng. Asian Pac J Cancer Prev 2008;9(4):543-8.

Aune D, De Stefani E, Ronco A, Boffetta P, Deneo-Pellegrini H,

Acosta G, et al. Fruits, vegetables and the risk of cancer: A

multisite case-control study in Uruguay. Asian Pac J Cancer Prev

;10(3):419-28.

Hasan TN, Grace BL, Shafi G, Al-Hazzani AA, Alshatwi AA. Antiproliferative

effects of organic extracts from root bark of Juglans

regia L. (RBJR) on MDA-MB-231 human breast cancer cells:

Role of Bcl-2/Bax, caspases and Tp53. Asian Pac J Cancer Prev

;12(2):525-30.

Rajendran S, Saravanan R, Ramalingam S, Hameed SA.

Antiproliferative and antioxidant activity of Gynandropsis pentaphylla

LINN on MCF-7 Cell line. Int J Pharm Pharm Sci 2014;6:561-5.

Stewart BW, Wild CP, editors. World Cancer Report 2014. Lyon,

France: International Agency for Research on cancer.

Subramaniyan V, Saravanan R, Baskaran D, Ramalalingam S.

In vitro free radical scavenging and anticancer potential of

Aristolochia indica L. Against Mcf-7cell line. Int J Pharm Pharm Sci

;7(6):392-6.

Hossain MA, Ismail Z, Rahman A, Kang SC. Chemical composition

and anti-fungal propertiesof the essential oils and crude extracts of

Orthosiphon stamineus Benth. Ind Crop Prod 2008;27:328-34.

Dharmaraj S, Hossain MA, Zhari S, Harn GL, Ismail Z. The use of

principal componentanalysis and selforganizing map to monitor

inhibition of calcium oxalate crystal growth by Orthosiphon stamineus

AQ1

Asian J Pharm Clin Res, Vol 10, Issue 3, 2017, 129-135

Saravanan et al.

extract. Chemometr Intell Lab Syst 2006;81(2):21-8.

Awale S, Tezuka Y, Banskota AH, Adnyana IK, Kadota S. Highly-oxygenated isopimarane-type diterpenes from Orthosiphon stamineus of Indonesia and their nitric oxide inhibitory activity. Chem Pharm Bull (Tokyo) 2003;51(3):268-75.

Akowuah GA, Ismail Z, Norhayati I, Sadikun A. The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity. Food Chem 2005;93:311-7.

Tezuka Y, Stampoulis P, Banskota AH, Awale S, Tran KQ, Saiki I, et al. Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chem Pharm Bull (Tokyo) 2000;48(11):1711-9.

Matsubara T, Bohgaki T, Watarai M, Suzuki H, Ohashi K, Shibuya H. Antihypertensive actions of methylripariochromene A from Orthosiphon aristatus, an Indonesian traditional medicinal plant. Biol Pharm Bull 1999;22(10):1083-8.

Gamble JS. Flora of the Presidency of Madras. Vol. 2. Dehradun: Bishen Singh Mahendra Pal Singh Dehra Dun; 2008. p. 1115.

Manjamalai A, Narala Y, Haridas A, Grace VM. Antifungal, anti-inflammatory and GC-MS analysis of methanolic extract of Plectranthus amboinicus leaf. Int J Curr Pharm Res 2011;2:129-36.

Harbone JB. Phytochemical Methods. 2nd ed. Bandung: Padmawinata; 1987.

Harbone JB. Phytochemical Methods. 3rd ed. London: Chapman and Hill; 1998.

Ayoola GA. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res 2008;7(3):1019-24.

Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100(1-2):72-9.

Schuier M, Sies H, Illek B, Fischer H. Cocoa-related flavonoids inhibit CFTR-mediated chloride transport across T84 human colon epithelia. J Nutr 2005;135(10):2320-5.

Cheng JH, Hung CF, Yang SC, Wang JP, Won SJ, Lin CN. Synthesis and cytotoxic, anti-inflammatory, and anti-oxidant activities of 2’, 5’-dialkoxylchalcones as cancer chemopreventive agents. Bioorg Med Chem 2008;16(15):7270-6.

Ravichandran N, Rajalakshmi P, Raj CD, Arun KP, Brindha P. Standardization and in vitro cytotoxic studies on Narasimha lehiyam: A potent anticancer Siddha drug. Asian J Chem 2014;26(12);3692-6.




About this article

Title

IN VITRO CYTOTOXIC AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES ON ORTHOSIPHON STAMINEUS BENTH. (LEAF) AGAINST MCF–7 CELL LINES

DOI

10.22159/ajpcr.2017.v10i3.15575

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 129-135

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

145 Views | 125 Downloads

Authors & Affiliations

Renuka Saravanan
SASTRA UNIVERSITY
India

Brindha Pemiah
SASTRA University
India

Mahesh Narayanan
SASTRA University
India

Sivakumar Ramalingam
SASTRA University
India


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