GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE

  • Renuka Saravanan Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.
  • Brindha Pemaiah Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India
  • Mahesh Narayanan Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.
  • Sivakumar Ramalingam Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.

Abstract

 

 Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.

Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH).

Results: Leaves extracts with different solvents revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, and tannins. GC-MS analysis of ethyl acetate of the plant leaves showed the presence of n-hexadecanoic acid. The IC50 value of the ethyl acetate extract was found to be 90.2 μg/ml on MCF-7 cell line, and the extract was found to possess significant DPPH free radical scavenging activity.

Conclusion: From the results, we conclude that the C. gynandra extract possesses antioxidant and antiproliferating activity against MCF-7 cells.

Keywords: Anticancer, Cleome gynandra, Palmitic acid, Phytol, phenol, 1, 1-diphenyl-2-picrylhydrazyl and 3-[4, 5-dimethylthiazol-2-yl]2, 5- diphenyltetrazolium bromide.

Author Biographies

Renuka Saravanan, Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.
Department Of Chemistry and Biosciences
Brindha Pemaiah, Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India
CARISM (Centre for Advanced Research and Indian System of Medicine)
Mahesh Narayanan, Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.
Department Of Chemistry and Biosciences
Sivakumar Ramalingam, Department of Chemistry & Biosciences, SRC, SASTRA University, Kumbakonam - 612 001, Tamil Nadu, India.
Department Of Chemistry and Biosciences

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Saravanan, R., B. Pemaiah, M. Narayanan, and S. Ramalingam. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 84-89, doi:10.22159/ajpcr.2017.v10i10.19343.
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