IN VITRO ANTIMALARIAL ACTIVITY OF CHLOROFORM, N-BUTANOL, AND ETHYL ACETATE FRACTIONS OF ETHANOL EXTRACTS OF CARTHAMUS TINCTORIUS LINN. FLOWERS


Rini Hamsidi, Aty Widyawaruyanti, Achmad Fuad Hafid, Wiwied Ekasari, Henny Kasmawati, Nur Illiyyin Akib, Wahyuni Wahyuni, Hajrul Malaka M

Abstract


Objective: This objective of this research was to study in vitro antimalarial activity of chloroform, n-butanol, and ethyl acetate fractions of ethanol extracts of Carthamus tinctorius Linn. flowers from Asteraceae family which empirically been used as traditional medication by people in South Sulawesi to heal measles.

Methods: Fractionation was conducted using chloroform, n-butanol, and ethyl acetate. Determination of antimalarial activity was performed by in vitro test using the 24-well microplate and the candle-jar method. Breeding is done in a petri-dish and done aseptically. Plasmodium falciparum 3D7 culture obtained from frozen deposits in-thawing and bread from Pharmacy Laboratory of Airlangga University, Surabaya, Indonesia. Blood sample with a density of over 2000 was employed. Serial decreasing concentrations of the crude extract of chloroform, butanol, and ethyl acetate fraction were tested for antimalarial activity. The following concentrations were used; 100; 10; 1.0; 0.1; and 0.01 (mg/mL). Negative controls used dimethyl sulfoxide (DMSO) diluted in the same manner as diluting materials above test, to obtain final DMSO concentration is not more than 0.5%. Mixture and suspension test parasites (= test preparation) are then inserted into the candle-jar and incubated in a CO2 incubator at a temperature of 37°C for 48 h. After incubation for 48 h made a thin blood smear on glass object. Smear dried at room temperature, fixed with methanol, then, once dry stained with Giemsa and counted under a microscope parasitemianya with 1000 times magnification. Calculations performed on 5000’s erythrocytes.

Results: Results showed that chloroform and n-butanol fraction cannot inhibit parasitemia >50%, but ethyl acetate fraction can inhibit parasitemia >50% with the highest inhibition at 100 μg/mL of 94.48%.

Conclusion: Ethyl acetate fraction is highly active as antimalarial with an IC50 of 1.25 μg/mL.


Keywords


Antimalarial activity, In vitro, Plasmodium falciparum 3D7, Carthamus tinctorius Linn.

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References


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About this article

Title

IN VITRO ANTIMALARIAL ACTIVITY OF CHLOROFORM, N-BUTANOL, AND ETHYL ACETATE FRACTIONS OF ETHANOL EXTRACTS OF CARTHAMUS TINCTORIUS LINN. FLOWERS

Keywords

Antimalarial activity, In vitro, Plasmodium falciparum 3D7, Carthamus tinctorius Linn.

DOI

10.22159/ajpcr.2018.v11i2.15856

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 121-123

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

34 Views | 74 Downloads

Authors & Affiliations

Rini Hamsidi
Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia.
Indonesia

Aty Widyawaruyanti
Department of Pharmacognosy and Phytochemistry, Airlangga University, Surabaya, Indonesia.
Indonesia

Achmad Fuad Hafid
Department of Pharmacognosy and Phytochemistry, Airlangga University, Surabaya, Indonesia.
India

Wiwied Ekasari
Department of Pharmacognosy and Phytochemistry, Airlangga University, Surabaya, Indonesia.
Indonesia

Henny Kasmawati
Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia.
Indonesia

Nur Illiyyin Akib
Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia.
Indonesia

Wahyuni Wahyuni
Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia.
Indonesia

Hajrul Malaka M
Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia.
Indonesia


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