ANTIHEPATITIS C VIRUS ACTIVITY OF INDONESIAN MAHOGANY (TOONA SURENI)


Achmad Fuad Hafid, Tutik Sri Wahyuni, Lidya Tumewu, Evhy Apryani, Adita Ayu Permanasari, Myrna Adianti, Chie Aoki Utsubo, Aty Widyawaruyanti, Maria Inge Lusida, Soetjipto Soetjipto, Hiroyuki Fuchino, Nobuo Kawahara, Hak Hotta

Abstract


 Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC50) value of 13.9 ± 1.6 μg/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract.

Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant.

Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC50 value of 1.7 μg/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC50 value of 29.90 μg/ml and 7.68 μg/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC50 value of 15.9 μg/ml.

Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.


Keywords


Toona sureni, Leaves extract, Polyphenols, Antihepatitis C virus, Gallic acid.

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About this article

Title

ANTIHEPATITIS C VIRUS ACTIVITY OF INDONESIAN MAHOGANY (TOONA SURENI)

Keywords

Toona sureni, Leaves extract, Polyphenols, Antihepatitis C virus, Gallic acid.

DOI

10.22159/ajpcr.2018.v11i2.18126

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 87-90

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Achmad Fuad Hafid
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Tutik Sri Wahyuni
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Lidya Tumewu
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Evhy Apryani
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Adita Ayu Permanasari
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Myrna Adianti
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Chie Aoki Utsubo
Department of International Health, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
Japan

Aty Widyawaruyanti
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Maria Inge Lusida
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Soetjipto Soetjipto
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Indonesia

Hiroyuki Fuchino
Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
Japan

Nobuo Kawahara
Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
Japan

Hak Hotta
Department of Oral Vaccine and Drug Development, Kobe University Graduate School of Health Sciences, 1-5-6 Minatojima-minamimachi, Chou-ku, Kobe 650-0047, Japan.
Japan


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