GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY


S C Joshi, Utkarsh Kaushik, Aproova Upadhyaya, Priyanka Sharma

Abstract


ABSTRACT
Objective: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. Silver nanoparticles
are usually synthesized by chemicals and physical method, which are quite toxic and flammable in nature. This study deals with an environment
friendly biosynthesis process of antibacterial silver nanoparticles using Momordica charantia fruit.
Methods: AgNO3 (5 mM) was allowed to react with fruit extract of M. charantia. Biosynthesis of AgNPs was optimized by changing temperature,
pH, and solvent. The silver nanoparticles so formed were characterized using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infrared
spectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscope (AFM), and scanning electron microscopy (SEM).
Results: UV-VIS spectra show absorption peak between 420 and 430 nm. The FTIR analysis showed the alcoholic, lactam, and nitro group present
in the plant extract, which were responsible for the reduction in AgNPs. The SEM images showed the size distribution of the nanoparticles and the
average size was found to be 50-100 nm. By DLS analysis and AFM analysis, average sizes of the silver nanoparticles were of 150 nm. The results of
these analyses confirmed the formation of silver nanoparticles. Silver nanoparticles were tested against Bacillus cereus and Staphylococcus epidermidis
strains using disc diffusion method and were found to be effective.
Conclusion: Silver nanoparticles so synthesized in this study using fruit extract of M. charantia are simple, easy, and effective technique of nanoparticles
production.
Keywords: Silver nanoparticles, Momordica charantia, Optimization, Antibacterial, Atomic force microscope, Scanning electron microscopy.


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About this article

Title

GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY

DOI

10.22159/ajpcr.2017.v10i3.16009

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 196-200

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

137 Views | 144 Downloads

Authors & Affiliations

S C Joshi
Department of Zoology, University of Rajasthan, Jaipur
India

Utkarsh Kaushik
Department of Zoology, University of Rajasthan, Jaipur
India

Aproova Upadhyaya
Department of Zoology, University of Rajasthan, Jaipur
India

Priyanka Sharma
Department of Zoology, University of Rajasthan, Jaipur
India


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