ANTI-ATHEROSCLEROTIC ACTIVITY OF ELAEOCARPUS GANITRUS ROXB. IN CHOLESTEROL-FED RABBITS


Pratibha Kumari Jain, Priyanka Sharma, Suresh C Joshi

Abstract


 

 Objectives: Atherosclerosis was experimentally induced in New Zealand white male rabbits by cholesterol feeding for 120 days for investigating effects of 70% ethanolic Elaeocarpus ganitrus seed extract (EEGS) against atherosclerosis.

Methods: Anti-atherosclerotic activity of E. ganitrus extract was evaluated at a dose level of 250 and 500 mg/kg of body weight (p.o). On 121st day, the animals were sacrificed to collect the aorta for determining tissue lipid profile, antioxidant parameters, and histopathological changes. One-way ANOVA followed by Tukey’s multiple comparison test was used for the statistical analysis.

Results: Aorta of cholesterol-fed rabbits for 120 days showed marked elevation in total cholesterol, triglycerides, and phospholipids. EEGS extract treatment significantly (p≤0.01, ≤0.001) corrected disturbed lipid profile in cholesterol-fed rabbits in a dose-dependent manner during the 60 days of the treatment period. A significant reduction in lipid peroxidation and a considerable raise in glutathione, catalase, and superoxide dismutase levels (p≤0.01, ≤0.001) were accompanied by post-treatment with EEGS extract to cholesterol-fed rabbits. The histopathological modifications such as accumulation of foam cells, atheromatous plaque formation, and lumen size reduction supported the successful induction of atherosclerosis in rabbits. The treated rabbits showed significant protective effect by lowering the deposition of cholesterol and increasing the lumen size compared to cholesterol-fed group.

Conclusions: Phytoconstituents such as alkaloids, tannins, flavonoids, steroids, triterpenoids, and carbohydrates in the ethanolic extract may be attributed to effective anti-atherosclerotic and antioxidant activity of plant extract. Our study exhibited that EEGS extract could be a potent herbal therapeutic agent for the treatment of atherosclerosis and hypercholesterolemia-related diseases.


Keywords


Elaeocarpus ganitrus, Atherosclerosis, Hyperlipidemic, Phytoconstituents, Cholesterol, Ethanolic extract.

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References


Kuklina EV, Yoon PW, Keenan NL. Trends in high levels of low-density lipoprotein cholesterol in the United States, 1999-2006. JAMA 2009;302(19):2104-10.

Hayashi K, Mani V, Nemade A, Aguiar S, Postley JE, Fuster V, et al. Variations in atherosclerosis and remodeling patterns in aorta and carotids. J Cardiovasc Magn Reson 2010;12:10.

Harauma A, Murayama T, Ikeyama K, Sano H, Arai H, Takano R, et al. Mulberry leaf powder prevents atherosclerosis in Apo lipoprotein E-deficient mice. Biochem Biophys Res Commun 2007;358(3):751-6.

Niki E. Antioxidants and atherosclerosis. Biochem Soc Trans 2004;32:156-9.

Sharma BR, Oh J, Kim HA, Kim YJ, Jeong KS, Rhyu DY, et al. Anti-obesity effects of the mixture of Eriobotrya japonica and Nelumbo nucifera in adipocytes and high-fat diet-induced obese mice. Am J Chin Med 2015;43(4):681-94.

Lai CH, Tsai CC, Kuo WW, Ho TJ, Day CH, Pai PY, et al. Multi-strain probiotics inhibit cardiac myopathies and autophagy to prevent heart injury in high-fat diet-fed rats. Int J Med Sci 2016;13(4):277-85.

Khandelwal KR. Preliminary phytochemical screening. Practical Pharmacognosy. Pune: Niralli Parkashan; 2004.

Nain J, Garg K, Dhahiya S. Analgesic and anti-inflammatory activity of Elaeocarpus sphaericus leaf extract. Int J Pharm Pharma Sci 2012;4 Suppl 1:379-81.

Katavic PL, Venables DA, Rali T, Carroll AR. Indolizidine alkaloids with delta-opioid receptor binding affinity from the leaves of Elaeocarpus fuscoides. J Nat Prod 2007;70(5):872-5.

Hule AK, Shah AS, Gambhire MN, Juvekar AR. An evaluation of the ant diabetic effects of Elaeocarpus ganitrus in experimental animals. Indian J Pharmacol 2011;43(1):56-9.

Dadhich A, Jasuja ND, Chandra S, Sharma G. Antidepressant effects of fruit extract of Elaeocarpus ganitrus in force swim test. Int J Pharm Sci Res 2014;5:2807-12.

Singh RK, Bhattacharya SK, Acharya SB. Studies on extracts of Elaeocarpus sphaericus fruits on in vitro rat mast cells. Phytomedicine 2000;7(3):205-7.

Sakat SS, Wankhede SS, Juvekar AR, Mali VR, Bodhankar SL. Antihypertensive effect of aqueous extract of Elaeocarpus ganitrus Roxb. seeds in renal artery occluded hypertensive rats. Int J PharmTech Res 2009;1(3):779-82.

Kumar G, Karthik L, Rao KV. Phytochemical composition and in vitro antimicrobial activity of Bauhinia racemosa Lamk (Caesalpiniaceae). Int J Pharm Sci Res 2010;1:51-8.

Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. J Lab Clin Med 1953;41(3):486-92.

Gottfried SP, Rosenburg B. Improved manual spectrophotometric procedure for determination of serum triglycerides. Clin Chem 1973;19(9):1077-8.

Zilversmit DB, Davis AK. Microdetermination of plasma phospholipids by trichloroacetic acid precipitation. J Lab Clin Med 1950;35(1):155-60.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.

Claiborne A. Catalase activity In: Greenwald R, editor. Handbook of methods for oxygen radical research. Boca Raton. Florida: CRC Press; 1985. p. 283-4.

Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 1975;250(14):5475-80.

Das K, Samanta L, Chainy GB. A modified spectrophotometric assay for superoxide dismutase using nitrite formation by superoxide radicals. Indian J Biochem Biophy 2000;37(3):201-4.

Brown AW, Hang J, Dussault PH, Carr TP. Plant sterol and stanol substrate specificity of pancreatic cholesterol esterase. J Nutr Biochem 2010;21(8):736-40.

Ahmida MH, Abuzogaya MH. The effects of oral administration of green tea and ginger extracts on serum and hepatic lipid content in rats fed a hyperlipidemic diet. Pharm Lett 2009;1(1):56-64.

Dhandapani R. Hypolipidemic activity of Eclipta prostrata (L.) L. leaf extract in atherogenic diet induced hyperlipidemic rats. Indian J Exp Biol 2007;45(7):617-9.

Muthu AK, Sethupathy S, Manavalan R, Karar PK. Hypolipidemic effect of methanolic extract of Dolichos biflorus Linn. in high fat diet fed rats. Indian J Exp Biol 2005;43(6):522-5.

Saikrishna N, Ranganayakulu D, Gopisudheerkumar J, Vijaykumar G, Anusha M. Anti atherosclerotic activity of the ethanolic extract of the Butea monosperma leaves as alone and in combination with the atorvastatin. J Adv Drug Res 2010;1:20-6.

Kumar DS, Muthu KA. Ondium suffruticosum (Ging.) (Family: Violaceae) in rat fed with high fat diet. Int J Pharm Pharm Sci 2012;4(4):69-73.

Mateos R, Lecumberri E, Ramos S, Goya L, Bravo L. Determination of malondialdehyde (MDA) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress-application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants from fruits. J Chromatogr B 2005;827:76-82.

Sabina EP, Rasool M. Therapeutic efficacy of Indian ayurvedic herbal formulation triphala on lipid peroxidation, antioxidant status and inflammatory mediators TNF-α in adjuvant induce arthritic mice. Int J Biol Chem 2007;1:149-55.

Yassa N, Razavi BH, Hadjiakhoondi A. Free radical scavenging and lipid peroxidation activity of the Shahani black grape. Pak J Biol Sci 2008;11(21):2513-6.

Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of organo essential oil. Food Chem 2004;85:633-40.

Bansal MP, Jaswal S. Hypercholesterolemia induced oxidative stress is reduced in rats with diets enriched with supplement from Dunaliella salina algae. Am J Biomed Sci 2009;1:196-204.

Prasanna GS, Purnima A. Protective effect of leaf extract of Trichilia connaroides on hypercholesterolemia induced oxidative stress. Int J Pharmacol 2011;7:106-12.

Deepa B, Anuradha CV. Antioxidant potential of Coriandrum sativum L. seed extract. Indian J Exp Biol 2011;49(1):30-8.

Sethi J, Sood S, Seth S, Talwar A. Evaluation of hypoglycemic and antioxidant effect of Ocimum sanctum. Indian J Clin Biochem 2011;19(2):152-5.

Fang YZ, Yang S, Wu G. Free radicals, antioxidants and nutrition. Nutrition 2002;18(10):872-9.

Visavadiya NP, Narasimhacharya AV. Hypolipidemic and antioxidant activities of Asparagus racemosus in hypercholesteremic rats. Indian J Pharmacol 2005;37(6):376-80.

Luo XD, Basile MJ, Kennelly EJ. Polyphenolic antioxidants from the fruits of Chrysophyllum cainito L. (star apple). J Agric Food Chem 2002;50(6):1379-82.

Prabha SP, Ansil PN, Nitha A, Wills PJ, Latha MS. Anti-atherogenic activity of methanolic extract of Gardenia gummifera linn. on high fat diet induced atherosclerosis in rats. Int J Pharm Pharm Sci 2013;5(2):388-93.

Aikawa M, Libby P. The vulnerable atherosclerotic plaque: Pathogenesis and therapeutic approach. Cardiovasc Pathol 2004;13:125-38.

Afonso MS, Silva AM, Carvalho EB, Rivelli DP, Barros SB, Rogero MM, et al. Phenolic compounds from rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in diet-induced hypercholesterolemic rats. Nutr Metab 2013;10(1):1-9.

Katan MB, Grundy SM, Jones P, Law M, Miettinen T, Paoletti R, et al. Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc 2003;78:965-978.

Schneider M, Sachett A, Schönell AP, Ibagy E, Fantin E, Bevilaqua F, et al. Hypoglycemic and hypolipidemic effects of Solidago chilensis in rats. Rev Bras Farmacogn 2015;25(3):258-63.

Wua SJ, Lean TN. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT-Food Sci Technol 2008;41:323-30.

Sathishsekar D, Subramanian S. Antioxidant properties of Momordica charantia (bitter gourd) seeds on streptozotocin induced diabetic rats. Asia Pac J Clin Nutr 2005;14(2):153-8.




About this article

Title

ANTI-ATHEROSCLEROTIC ACTIVITY OF ELAEOCARPUS GANITRUS ROXB. IN CHOLESTEROL-FED RABBITS

Keywords

Elaeocarpus ganitrus, Atherosclerosis, Hyperlipidemic, Phytoconstituents, Cholesterol, Ethanolic extract.

DOI

10.22159/ajpcr.2017.v10i12.20111

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 80-84

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

19 Views | 38 Downloads

Authors & Affiliations

Pratibha Kumari Jain
Department of Zoology, Reproductive Toxicology Unit, Center for Advanced Studies, University of Rajasthan, Jaipur - 302 055, Rajasthan, India.
India

Priyanka Sharma
Department of Zoology, Reproductive Toxicology Unit, Center for Advanced Studies, University of Rajasthan, Jaipur - 302 055, Rajasthan, India.
India

Suresh C Joshi
Department of Zoology, Reproductive Toxicology Unit, Center for Advanced Studies, University of Rajasthan, Jaipur - 302 055, Rajasthan, India.
India


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